Human CX3CL1/Fractalking enzyme-linked immunoassay kit
Catalog# EH0213
Human CX3CL1/Fractalking enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
156.25~10000 pg/ml
概述
产品名称
Human CX3CL1/Fractalking enzyme-linked immunoassay kit
产品类型
Elisa Kit
灵敏度
7.47 pg/ml (50 μl)；16.03 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0138 | 0.0133 | 0.0136 | |
| 156.25 | 0.0427 | 0.0364 | 0.0396 | 0.0260 |
| 312.50 | 0.0842 | 0.0711 | 0.0777 | 0.0641 |
| 625.00 | 0.1921 | 0.1773 | 0.1847 | 0.1712 |
| 1250.00 | 0.5697 | 0.4696 | 0.5197 | 0.5061 |
| 2500.00 | 1.3220 | 1.2500 | 1.2860 | 1.2725 |
| 5000.00 | 2.5240 | 2.4520 | 2.4880 | 2.4745 |
| 10000.00 | 3.5110 | 3.5180 | 3.5145 | 3.5010 |
精度
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average () | 285.8 | 1505.4 | 4447.7 | 294.6 | 1388.2 | 4357.8 |
| Standard Deviation | 21.7 | 80.1 | 223.3 | 12.3 | 74.3 | 153.6 |
| Coefficient of Variation (%) | 7.6 | 5.3 | 5.0 | 4.2 | 5.4 | 3.5 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
加标回收
健康人血清加入 3 个不同浓度水平的人 CX3CL1/Fractalking ，未加人 CX3CL1/Fractalking 的血清作为本底，计算回收率。回收率的范围从 79 % 至 128 %，平均回收率为 114%。
样本值
| Sample Matrix | Sample Evaluated | Range () | Detectable (%) | Mean of Detectable () |
|---|---|---|---|---|
| Serum | 30 | 516.05-2692.08 | 100 | 1625.88 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
实验类型
Sandwich Elisa
种属反应性
Human
检测方法
Colorimetric
操作时长
120min
标准曲线梯度
7点
孵育次数
2
可检测样本
可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。
加样体积
50 μl/10 μl
产品特性
储存条件
存储于 2-8 ℃
靶点
功能
Fractalkine (CX3CL1) is the only member of the CX3C chemokine, composed of 373 amino acids, and exists in membrane-bound and secretory forms. The membrane-bound type is composed of chemotaxis domain, mucin-like stem-like structure, transmembrane domain and intracellular domain, which can be induced and expressed by inflammatory factors. The secretory form is produced by proteolysis and has chemolotaxis. It is expressed in mammalian brain, lung, heart and other organs, and neurons and glial cells can secrete this protein.