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Human Caspase-3/CASP3, C-His Tag
Human Caspase-3/CASP3, C-His Tag Protein

Human Caspase-3/CASP3, C-His Tag Protein

DATA SHEET
MSDS
COA

Rmb: 845 1300 特惠 1300

产品规格

20μg

50μg

100μg

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Catalog# HA211516

Human Caspase-3/CASP3, C-His Tag Protein

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MSDS_HUABIO.pdf
MSDS_HUABIO.pdf
No MSDS Found

Product Information

Product name

Human Caspase-3/CASP3, C-His Tag Protein

Catalog No.

HA211516

Predicted molecular mass

30.9 kD

Protein construction description

A DNA sequence encoding the human Caspase-3/CASP3 protein (P42574) (Ser 29-His 277, C163A) was expressed with a His tag at the C-terminus.

Accession

P42574

Protein construction

Caspase-3/CASP3 (29-277,C163A) His

Source

E.coli

Bio Activity

Testing in progress.

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.

Species

Human

Background

Caspase-3 is a caspase protein that interacts with caspase-8 and caspase-9. It is encoded by the CASP3 gene. CASP3 orthologs have been identified in numerous mammals for which complete genome data are available. Unique orthologs are also present in birds, lizards, lissamphibians, and teleosts. Caspase-3 shares many of the typical characteristics common to all currently-known caspases. For example, its active site contains a cysteine residue (Cys-163) and histidine residue (His-121) that stabilize the peptide bond cleavage of a protein sequence to the carboxy-terminal side of an aspartic acid when it is part of a particular 4-amino acid sequence. This specificity allows caspases to be incredibly selective, with a 20,000-fold preference for aspartic acid over glutamic acid. A key feature of caspases in the cell is that they are present as zymogens, termed procaspases, which are inactive until a biochemical change causes their activation. Each procaspase has an N-terminal large subunit of about 20 kDa followed by a smaller subunit of about 10 kDa, called p20 and p10, respectively.

Usage guide

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped with blue ice. Bulk packages of recombinant proteins are provided as frozen liquid which are shipped with dry ice.

Storage

Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.