Human MAP4K1/HPK1, N-Twin Strep, C-His, Flag Tag Protein
Catalog# HA210723
Human MAP4K1/HPK1, N-Twin Strep, C-His, Flag Tag Protein
Product Information
Product name
Human MAP4K1/HPK1, N-Twin Strep, C-His, Flag Tag Protein
Catalog No.
HA210723
Predicted molecular mass
21 kD
Protein construction description
A DNA sequence encoding the human MAP4K1/HPK1 protein (Q92918) (Ser 324-Ser 457) was expressed with a Twin strep tag at the N-terminus and both His, Flag tag at the C-terminus.
Accession
Q92918
Protein construction
Source
E.coli
Bio Activity
Testing in progress.
Purity
>95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Formulation
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
Species
Human
Background
Enables ATP binding activity and MAP kinase kinase kinase kinase activity. Involved in several processes, including JNK cascade; cellular response to phorbol 13-acetate 12-myristate; and protein phosphorylation. Located in membrane. Serine/threonine-protein kinase, which may play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation. Able to autophosphorylate 5. Together with CLNK, it enhances CD3-triggered activation of T-cells and subsequent IL2 production.
Usage guide
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped with blue ice. Bulk packages of recombinant proteins are provided as frozen liquid which are shipped with dry ice.
Storage
Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
Reconstitution
Reconstitute at 250 μg/ml in sterile water.
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