Formyl-Histone H3 (K122) Recombinant Rabbit Monoclonal Antibody [PSH27-72]

Rmb: 699 1600 特惠 1600

产品规格

50μl

100μl

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Catalog# HA724717

Formyl-Histone H3 (K122) Recombinant Rabbit Monoclonal Antibody [PSH27-72]

Application

WB
IHC-P

Reactivity

Human
Mouse
Rat

Conjugation

unconjugated

概述

产品名称

Formyl-Histone H3 (K122) Recombinant Rabbit Monoclonal Antibody [PSH27-72]

抗体类型

Recombinant Rabbit monoclonal Antibody

种属反应性

Human, Mouse, Rat

验证应用

WB, IHC-P

靶点分子量

Predicted band size: 15 kDa

阳性对照

A549 (Human lung adenocarcinoma cell) cell lysate, HeLa (Human cervical adenocarcinoma cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate.

偶联

unconjugated

克隆号

PSH27-72

反应性数据

Tested

已验证（内部验证通过）

Published

文献已报道（未内部验证，但有文献支持）

Predicted

预测可反应（基于高同源性）

Not recommended

不推荐（内部验证未通过）

	WB	IHC-P
human
mouse
rat

产品特性

形态

Liquid

浓度

批次浓度查询

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

WB
1:500

IHC-P
1:500

靶点

功能

Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fibers. Two molecules of each of the four core histones (H2A, H2B, H3 and H4) form the octamer, which is comprised of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Human and mouse Histone H4 are subject to methylation at Lys 20, a modification that may be necessary for select DNA transactions or chromatin state transitions.

背景文献

1. Wilson J.P. et. al. Proteomic analysis of fatty-acylated proteins in mammalian cells with chemical reporters reveals S-acylation of histone H3 variants. Mol. Cell. Proteomics 10:M110.001198-M110.001198(2011).

亚细胞定位

Chromosome, Nucleosome core, Nucleus.

UNIPROT

SwissProt: P68431 Human

SwissProt: P84243 Human

SwissProt: Q16695 Human

SwissProt: Q71DI3 Human

SwissProt: Q6NXT2 Human

别名

H3 histone family member E pseudogene antibody

H3 histone family, member A antibody

H3/A antibody

H31_HUMAN antibody

H3F3 antibody

H3FA antibody

Hist1h3a antibody

HIST1H3B antibody

HIST1H3C antibody

HIST1H3D antibody

展开

图片

Western blot analysis of Formyl-Histone H3 (K122) on different lysates with Rabbit anti-Formyl-Histone H3 (K122) antibody (HA724717) at 1/500 dilution.

Lane 1: A549 (Human lung adenocarcinoma cell) cell lysate
Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 3: NIH/3T3 (Mouse fibroblast) cell lysate

Lysates/proteins at 20 µg/Lane.
Exposure time: 3 minutes ; ECL: K1802

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724717, 1/500 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 15 kDa
Observed band size: 15 kDa
Application: Immunohistochemistry (IHC-P)

Species: Human
Tissue: Colon
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724717, 1/500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
Application: Immunohistochemistry (IHC-P)

Species: Mouse
Tissue: Colon
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724717, 1/500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
Application: Immunohistochemistry (IHC-P)

Species: Rat
Tissue: Colon
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724717, 1/500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Formyl-Histone H3 (K122) Recombinant Rabbit Monoclonal Antibody [PSH27-72]

Rmb: 699 1600 特惠 1600

产品规格

联系我们

概述

产品名称

抗体类型

种属反应性

验证应用

靶点分子量

阳性对照

偶联

克隆号

反应性数据

产品特性

形态

浓度

存放说明

存储缓冲液

亚型

纯化方式

应用稀释度

靶点

功能

背景文献

亚细胞定位

UNIPROT

别名

图片

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