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HRP Conjugated Goat anti-Rabbit IgG UltraPolymer Antibody

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Catalog# HA1119

HRP Conjugated Goat anti-Rabbit IgG UltraPolymer Antibody

Application

  • IHC-P

Reactivity

  • Rabbit

Conjugation

  • HRP

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

HRP Conjugated Goat anti-Rabbit IgG UltraPolymer Antibody

抗体类型

Goat Polyclonal Antibody

免疫原

Purified Rabbit IgG, whole molecule.

种属反应性

Rabbit

验证应用

IHC-P

偶联

HRP

产品特性

形态

Liquid

存放说明

Store at 2-8 ℃.

存储缓冲液

TBS, 1% (w/v) BSA, 0.1% Proclin 150, 10% heat-inactivated animal serum.

亚型

IgG

纯化方式

Affinity purified using solid phase Rabbit IgG.

应用稀释度

  • IHC-P

  • Ready to Use

靶点

功能

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells. UltraPolymer Goat anti-Rabbit IgG (H&L) conjugated to HRP, affinity purified, min x w/ human, mouse or rat serum.

背景文献

暂无

图片

  • Immunohistochemical analysis of paraffin-embedded Human thyroid tissue using anti-NRF1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The detection was performed using an Goat anti-Rabbit IgG (H&L) - HRP (<a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>). Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded Human thyroid tissue using anti-NRF1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The detection was performed using an Goat anti-Rabbit IgG (H&L) - HRP (HA1119). Counter stained with hematoxylin.

  • Analysis of HeLa cells labeling Ki67 with Rabbit anti-Ki67 antibody (<a href="/products/HA721115" style="font-weight: bold;text-decoration: underline;">HA721115</a>) at 1/250 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ki67 antibody (<a href="/products/HA721115" style="font-weight: bold;text-decoration: underline;">HA721115</a>) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. The detection was performed using an Goat anti-Rabbit IgG (H&L) - HRP (<a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>).

    Analysis of HeLa cells labeling Ki67 with Rabbit anti-Ki67 antibody (HA721115) at 1/250 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ki67 antibody (HA721115) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. The detection was performed using an Goat anti-Rabbit IgG (H&L) - HRP (HA1119).

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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