Histone H3 Rabbit Polyclonal Antibody

RMB: 900 特惠 1500 1500

产品规格

50μl

100μl

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Catalog# HA500298

Histone H3 Rabbit Polyclonal Antibody

Application

Reactivity

Zebrafish
Human
Mouse

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

Histone H3 Rabbit Polyclonal Antibody

抗体类型

Rabbit Polyclonal Antibody

免疫原

Synthetic peptide within Zebrafish Histone H3 aa 1-50 / 136.

种属反应性

Zebrafish, Human, Mouse

验证应用

分子量

Predicted band size: 15 kDa

阳性对照

Zebrafish tissue lysates.

偶联

unconjugated

RRID

AB_3071393

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Immunogen affinity purified.

应用稀释度

WB
1:500-1:5,000

发表文章中的应用

WB	查看 1 篇文献如下

发表文章中的种属

Human

查看 1 篇文献如下

靶点

功能

Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

背景文献

1. Jacob Y.et.al.Selective methylation of histone H3 variant H3.1 regulates heterochromatin replication.Science 343:1249-1253(2014).

亚细胞定位

Nucleus,Chromosome.

UNIPROT

SwissProt: Q6PI20 Zebrafish

SwissProt: Q4QRF4 Zebrafish

SwissProt: B2GSF5 Zebrafish

别名

H3 histone family member E pseudogene antibody

H3 histone family, member A antibody

H3/A antibody

H31_HUMAN antibody

H3F3 antibody

H3FA antibody

Hist1h3a antibody

HIST1H3B antibody

HIST1H3C antibody

HIST1H3D antibody

展开

图片

Western blot analysis of Histone H3 on zebrafish tissue lysates with Rabbit anti-Histone H3 antibody (HA500298) at 1/5,000 dilution.

Lysates/proteins at 10 µg/Lane.

Predicted band size: 15 kDa
Observed band size: 15 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500298) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:

Linear ubiquitination regulates the KSHV replication and transcription activator protein to control infection

Author: Luan Yi,et al

PMID: 38951495
期刊: Nature Communications
- 应用: WB
- 反应种属: Human
- 发表时间: 2024 Jul
- Citation

Histone H3 Rabbit Polyclonal Antibody

RMB: 900 特惠 1500 1500

产品规格

联系我们

概述

产品名称

抗体类型

免疫原

种属反应性

验证应用

分子量

阳性对照

偶联

RRID

产品特性

形态

存放说明

存储缓冲液

亚型

纯化方式

应用稀释度

发表文章中的应用

发表文章中的种属

靶点

功能

背景文献

亚细胞定位

UNIPROT

别名

图片

引文

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