GAD67 Recombinant Antibody [PSH08-30] - Rabbit IgG (Chimeric)
Rmb: 666 特惠 1500
Catalog# HA724362
GAD67 Recombinant Antibody [PSH08-30] - Rabbit IgG (Chimeric)
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WB
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IHC-P
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IF-Tissue
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mIHC
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Human
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Mouse
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Rat
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HA751895
不含抗保成分
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unconjugated
概述
产品名称
GAD67 Recombinant Antibody [PSH08-30] - Rabbit IgG (Chimeric)
抗体类型
Recombinant Chimeric Antibody
免疫原
Recombinant protein within human GAD67 aa 1-594.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P, IF-Tissue, mIHC
靶点分子量
Predicted band size: 67 kDa
阳性对照
Mouse brain tissue lysate, Rat brain tissue lysate.
偶联
unconjugated
克隆号
PSH08-30
反应性数据
已验证（内部验证通过）
文献已报道（未内部验证，但有文献支持）
预测可反应（基于高同源性）
不推荐（内部验证未通过）
| WB | IHC-P | IHC-Fr | IF-Tissue | mIHC | |
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| human |
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| mouse |
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| rat |
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产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:5,000
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IHC-P
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1:1,000
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IF-Tissue
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1:500
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mIHC
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1:1,000
靶点
功能
There are two forms of glutamic acid decarboxylases (GADs) that are found in the brain: GAD-65 (also known as GAD2) and GAD-67 (also known as GAD1, GAD or SCP). GAD-65 and GAD-67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate limiting step in the production of GABA (g-aminobutyric acid) from L-glutamic acid. Although both GADs are found in the brain, GAD-65 localizes to synaptic vesicle membranes in nerve terminals, while GAD-67 is distributed throughout the cell. GAD-67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD-65 will transiently activate to assist in GABA production. The loss of GAD-65 is detrimental and can impair GABA neurotransmission, however the loss of GAD-67 is lethal. Due to alternative splicing, two isoforms exist for GAD-67, the predominant GAD-67 form and the minor GAD-25 form. GAD-25 is not expressed in brain but can be found in a variety of endocrine tissues.
背景文献
1. Li JT et al. Repeated Blockade of NMDA Receptors During Adolescence Impairs Reversal Learning and Disrupts GABAergic Interneurons in Rat Medial Prefrontal Cortex. Front Mol Neurosci 9:17 (2016).
2. Fu Q et al. MHC-I promotes apoptosis of GABAergic interneurons in the spinal dorsal horn and contributes to cancer induced bone pain. Exp Neurol 286:12-20 (2016).
亚细胞定位
Cytoplasm, Plasma Membrane.
别名
67 kDa glutamic acid decarboxylase antibody
CPSQ1 antibody
DCE1 antibody
DCE1_HUMAN antibody
EC 4.1.1.15 antibody
FLJ45882 antibody
GAD 67 antibody
GAD antibody
GAD-67 antibody
GAD1 antibody
展开图片
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mIHC analysis of mouse cerebellum tissue (paraffin-embedded) with GAD67 (HA724362, 1/1,000), QKI (HA601377, 1/500) and CNPase (ET1702-46, 1/2,000).
The immunostaining was performed with the IRISKitCmTSA Kit (900802). DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner. -
mIHC analysis of rat cerebellum tissue (paraffin-embedded) with GAD67 (HA724362, 1/1,000), QKI (HA601377, 1/500) and CNPase (ET1702-46, 1/2,000).
The immunostaining was performed with the IRISKitCmTSA Kit (900802). DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner. -
Application: Immunofluorescence (IF-tissue)
Species: Mouse
Tissue: Hippocampus
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× PBST
Endogenous peroxidase blocking: 3% H2O2, 10 minutes.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: GAD67, 1/500 (HA724362, Rabbit, Green), VGLUT1, 1/500 (HA601449, Guinea pig, Red), overnight at 4℃.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), Goat anti-Guinea pig IgG (iFluor™ 594, HA1143), 1.5 hours at room temperature. -
Application: Immunofluorescence (IF-tissue)
Species: Rat
Tissue: Hippocampus
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× PBST
Endogenous peroxidase blocking: 3% H2O2, 10 minutes.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: GAD67, 1/500 (HA724362, Rabbit, Green), VGLUT1, 1/500 (HA601449, Guinea pig, Red), overnight at 4℃.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), Goat anti-Guinea pig IgG (iFluor™ 594, HA1143), 1.5 hours at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Cerebellum
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724362, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Cerebellum
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724362, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Cerebellum
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724362, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Hippocampus
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724362, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Hippocampus
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724362, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
☑ Relative expression (RE)
Western blot analysis of GAD67 on different lysates with Rabbit anti-GAD67 antibody (HA724362) at 1/5,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Mouse liver tissue lysate (negative)
Lane 3: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 3 minutes; ECL: K1802
Mouse liver is a negative control (PMID: 33968199).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724362, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 67 kDa
Observed band size: 67 kDa
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