CNPase Recombinant Rabbit Monoclonal Antibody [JF10-25]
Catalog# ET1702-46
CNPase Recombinant Rabbit Monoclonal Antibody [JF10-25]
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WB
-
IHC-P
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IF-Tissue
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Human
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Mouse
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Rat
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Cynomolgus monkey
预测的种属支持售后
概述
产品名称
CNPase Recombinant Rabbit Monoclonal Antibody [JF10-25]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human CNPase aa 386-421 / 421.
种属反应性
Human, Mouse, Rat (Predicted: Cynomolgus monkey)
预测的种属支持售后
验证应用
WB, IHC-P, IF-Tissue
分子量
Predicted band size: 48 kDa
阳性对照
Mouse brain tissue lysate, mouse cerebrum tissue lysate, SHG-44, SH-SY5Y, N2A, mouse brain tissue, rat brain tissue, mouse cerebellum tissue.
偶联
unconjugated
克隆号
JF10-25
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
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1:1,000-1:2,000
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IHC-P
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1:500
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IF-Tissue
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1:100
靶点
功能
2',3'-cyclic nucleotide-3'-phosphodiesterase (CNPase) is a membrane-bound enzyme that can link tubulin to membranes and may regulate cytoplasmic microtubule distribution. CNPase acts as a microtubule-associated protein by promoting microtubule assembly; this activity resides in the C-terminus of the enzyme. CNPase is firmly associated with tubulin from brain tissue and thyroid cells and can be found at high concentrations in central nervous system myelin and in the outer segments of photoreceptors in the retina. Acute lead intoxication leads to disturbances in CNPase activity and the morphology of myelin.
背景文献
1. Chang S et al. Neuropsin Inactivation Has Protective Effects against Depressive-Like Behaviours and Memory Impairment Induced by Chronic Stress. PLoS Genet 12:e1006356 (2016).
2. Kuzdas-Wood D et al. Involvement of Peripheral Nerves in the Transgenic PLP-a-Syn Model of Multiple System Atrophy: Extending the Phenotype. PLoS One 10:e0136575 (2015).
序列相似性
Belongs to the 2H phosphoesterase superfamily. CNPase family.
亚细胞定位
Membrane, Melanosome.
别名
2' antibody
2'3' cyclic nucleotide 3' phosphodiesterase antibody
3'-cyclic-nucleotide 3'-phosphodiesterase antibody
CN37_HUMAN antibody
CNP 1 antibody
CNP antibody
CNP1 antibody
CNPase antibody
图片
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Immunofluorescence analysis of paraffin-embedded mouse cerebellum tissue labeling CNPase with Rabbit anti-CNPase antibody (ET1702-46) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1702-46, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
☑ Relative expression (RE)
Western blot analysis of CNPase on different lysates with Rabbit anti-CNPase antibody (ET1702-46) at 1/2,000 dilution.
Lane 1: Mouse cerebellum tissue lysate
Lane 2: Mouse liver tissue lysate (low expression)
Lane 3: Rat cerebellum tissue lysate
Lane 4: Rat liver tissue lysate (low expression)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 14 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-46) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-CNPase antibody (ET1702-46) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-46) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-CNPase antibody (ET1702-46) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-46) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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