GSK3 alpha Recombinant Rabbit Monoclonal Antibody [JE33-27]
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Catalog# HA722217
GSK3 alpha Recombinant Rabbit Monoclonal Antibody [JE33-27]
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WB
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IHC-P
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Human
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Mouse
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Rat
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Monkey
概述
产品名称
GSK3 alpha Recombinant Rabbit Monoclonal Antibody [JE33-27]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human GSK3 alpha aa 50-150.
种属反应性
Human, Mouse, Rat, Monkey
验证应用
WB, IHC-P
分子量
Predicted band size: 51 kDa
阳性对照
HeLa cell lysate, A431 cell lysate, A549 cell lysate, Jurkat cell lysate, HCT 116cell lysate, HEK-293 cell lysate, U-87 MG cell lysate, MCF7 cell lysate, MDA-MB-231 cell lysate, SK-Br-3 cell lysate, Neuro-2a cell lysate, NIH/3T3 cell lysate, C6 cell lysate, COS-1 cell lysate, human ovary cancer tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue.
偶联
unconjugated
克隆号
JE33-27
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:2,000
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IHC-P
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1:200-1:1,000
靶点
功能
Glycogen synthase kinase-3 alpha is an enzyme that in humans is encoded by the GSK3A gene. Glycogen synthase kinase 3-alpha EC 2.7.1.37 is a multifunctional protein serine kinase, homologous to Drosophila 'shaggy' (zeste-white3) and implicated in the control of several regulatory proteins including glycogen synthase and various transcription factors (e.g., JUN). It also plays a role in the WNT and phosphoinositide 3-kinase (especially PIK3CG) signaling pathways.
背景文献
1. Wang J et al. Quantitative phosphoproteomics reveals GSK3A substrate network is involved in the cryodamage of sperm motility. Biosci Rep. 2021 Oct
2. Zhang W et al. Active Ingredient Paeonol of Jijiu Huiyang Decoction Alleviates Isoproterenol-Induced Chronic Heart Failure via the GSK3A/PPARalpha Pathway. Oxid Med Cell Longev. 2023 Feb
亚细胞定位
Apical dendrite, axon, cytoplasm, cytosol, mitochondrion, neuronal cell body, nucleus, postsynapse, proximal dendrite.
别名
DKFZp686D0638 antibody
Glycogen synthase kinase 3 alpha antibody
Glycogen synthase kinase-3 alpha antibody
GSK 3 alpha antibody
GSK 3A antibody
GSK-3 alpha antibody
Gsk3a antibody
GSK3A_HUMAN antibody
GSK3alpha antibody
Serine/threonine protein kinase GSK3A antibody
图片
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Western blot analysis of GSK3 alpha on different lysates with Rabbit anti-GSK3 alpha antibody (HA722217) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: A431 cell lysate
Lane 3: A549 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: HCT 116cell lysate
Lane 6: HEK-293 cell lysate
Lane 7: U-87 MG cell lysate
Lane 8: MCF7 cell lysate
Lane 9: MDA-MB-231 cell lysate
Lane 10: SK-Br-3 cell lysate
Lane 11: Neuro-2a cell lysate
Lane 12: NIH/3T3 cell lysate
Lane 13: C6 cell lysate
Lane 14: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 51 kDa
Observed band size: 51 kDa
Exposure time: 42 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722217) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue with Rabbit anti-GSK3 alpha antibody (HA722217) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722217) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-GSK3 alpha antibody (HA722217) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722217) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-GSK3 alpha antibody (HA722217) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722217) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-GSK3 alpha antibody (HA722217) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722217) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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