Skip to content

Phospho-GSK3 alpha (S21) Recombinant Rabbit Monoclonal Antibody [PSH09-11]

RMB: 699 特惠 1600

产品规格

现在订购，【现货】

点击询价

联系我们

邮箱: sales@huabio.cn

电话: 0571-88062880

查找各地代理商

Catalog# HA723069

Phospho-GSK3 alpha (S21) Recombinant Rabbit Monoclonal Antibody [PSH09-11]

Application

  • WB

  • IHC-P

  • Dot Blot

Reactivity

  • Human

  • Mouse

概述

产品名称

Phospho-GSK3 alpha (S21) Recombinant Rabbit Monoclonal Antibody [PSH09-11]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic phospho-peptide corresponding to residues surrounding Ser21 of Human GSK3 alpha.

种属反应性

Human, Mouse

验证应用

WB, IHC-P, Dot Blot

分子量

Predicted band size: 51 kDa

阳性对照

A549 treated with 100nM Calyculin A for 30 minutes cell lysate, SH-SY5Y treated with 200nM Calyculin A and 1μM Okadaic Acid for 1 hour cell lysate, NIH/3T3 starved overnight cell lysate, NIH/3T3 starved overnight then treated with 100ng/mL PDGF for 5 minutes cell lysate, mouse testis tissue, human breast tissue, human colon cancer tissue.

偶联

unconjugated

克隆号

PSH09-11

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:2,000

  • IHC-P

  • 1:200-1:500

  • Dot Blot

  • 1:1,000

靶点

功能

Glycogen synthase kinase-3 alpha is an enzyme that in humans is encoded by the GSK3A gene. Glycogen synthase kinase 3-alpha EC 2.7.1.37 is a multifunctional protein serine kinase, homologous to Drosophila 'shaggy' (zeste-white3) and implicated in the control of several regulatory proteins including glycogen synthase and various transcription factors (e.g., JUN). It also plays a role in the WNT and phosphoinositide 3-kinase (especially PIK3CG) signaling pathways.

背景文献

1. Wang J et al. Quantitative phosphoproteomics reveals GSK3A substrate network is involved in the cryodamage of sperm motility. Biosci Rep. 2021 Oct

2. Zhang W et al. Active Ingredient Paeonol of Jijiu Huiyang Decoction Alleviates Isoproterenol-Induced Chronic Heart Failure via the GSK3A/PPARalpha Pathway. Oxid Med Cell Longev. 2023 Feb

亚细胞定位

Apical dendrite, axon, cytoplasm, cytosol, mitochondrion, neuronal cell body, nucleus, postsynapse, proximal dendrite.

UNIPROT

SwissProt: P49840 Human

SwissProt: Q2NL51 Mouse

别名

DKFZp686D0638 antibody

Glycogen synthase kinase 3 alpha antibody

Glycogen synthase kinase-3 alpha antibody

GSK 3 alpha antibody

GSK 3A antibody

GSK-3 alpha antibody

Gsk3a antibody

GSK3A_HUMAN antibody

GSK3alpha antibody

Serine/threonine protein kinase GSK3A antibody

图片

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-GSK3 alpha (S21) on different lysates with Rabbit anti-Phospho-GSK3 alpha (S21) antibody (<a href="/products/HA723069" style="font-weight: bold;text-decoration: underline;">HA723069</a>) at 1/2,000 dilution.<br /><br />Lane 1: A549 cell lysate<br />Lane 2: A549 treated with 100nM Calyculin A for 30 minutes cell lysate<br />Lane 3: SH-SY5Y cell lysate<br />Lane 4: SH-SY5Y treated with 200nM Calyculin A and 1μM Okadaic Acid for 1 hour cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 51 kDa<br />Observed band size: 51 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723069" style="font-weight: bold;text-decoration: underline;">HA723069</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-GSK3 alpha (S21) on different lysates with Rabbit anti-Phospho-GSK3 alpha (S21) antibody (HA723069) at 1/2,000 dilution.

    Lane 1: A549 cell lysate
    Lane 2: A549 treated with 100nM Calyculin A for 30 minutes cell lysate
    Lane 3: SH-SY5Y cell lysate
    Lane 4: SH-SY5Y treated with 200nM Calyculin A and 1μM Okadaic Acid for 1 hour cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 51 kDa
    Observed band size: 51 kDa

    Exposure time: 1 minute; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723069) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-GSK3 alpha (S21) on different lysates with Rabbit anti-Phospho-GSK3 alpha (S21) antibody (<a href="/products/HA723069" style="font-weight: bold;text-decoration: underline;">HA723069</a>) at 1/2,000 dilution.<br /><br />Lane 1: NIH/3T3 starved overnight cell lysate<br />Lane 2: NIH/3T3 starved overnight then treated with 100ng/mL PDGF for 5 minutes cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 51 kDa<br />Observed band size: 51 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723069" style="font-weight: bold;text-decoration: underline;">HA723069</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-GSK3 alpha (S21) on different lysates with Rabbit anti-Phospho-GSK3 alpha (S21) antibody (HA723069) at 1/2,000 dilution.

    Lane 1: NIH/3T3 starved overnight cell lysate
    Lane 2: NIH/3T3 starved overnight then treated with 100ng/mL PDGF for 5 minutes cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 51 kDa
    Observed band size: 51 kDa

    Exposure time: 3 minutes; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723069) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Phospho-GSK3 alpha (S21) antibody (<a href="/products/HA723069" style="font-weight: bold;text-decoration: underline;">HA723069</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723069" style="font-weight: bold;text-decoration: underline;">HA723069</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Cell treatment (CT)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Phospho-GSK3 alpha (S21) antibody (HA723069) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723069) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Phospho-GSK3 alpha (S21) antibody (<a href="/products/HA723069" style="font-weight: bold;text-decoration: underline;">HA723069</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723069" style="font-weight: bold;text-decoration: underline;">HA723069</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Phospho-GSK3 alpha (S21) antibody (HA723069) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723069) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Phospho-GSK3 alpha (S21) antibody (<a href="/products/HA723069" style="font-weight: bold;text-decoration: underline;">HA723069</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723069" style="font-weight: bold;text-decoration: underline;">HA723069</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Phospho-GSK3 alpha (S21) antibody (HA723069) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723069) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Dot blot analysis of Phospho-GSK3 alpha (S21) on different peptides with Rabbit anti-Phospho-GSK3 alpha (S21) antibody (<a href="/products/HA723069" style="font-weight: bold;text-decoration: underline;">HA723069</a>) at 1/1,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution for 1 hour at room temperature.<br /><br />Lane 1: Phospho-GSK3 alpha (S21) peptide (positive)<br />Lane 2: Unmodified GSK3 alpha peptide (negative)<br />Lane 3: Phospho-GSK3 beta (S9) peptide (negative)<br />Lane 4: Unmodified GSK3 beta peptide (negative)<br /><br />Proteins loading: 100ng, 25ng, 5ng;<br /><br />Blocking and dilution buffer: 5% NFDM/TBST;<br /><br />Exposure time: 10 seconds; ECL: K1801.

    Dot blot analysis of Phospho-GSK3 alpha (S21) on different peptides with Rabbit anti-Phospho-GSK3 alpha (S21) antibody (HA723069) at 1/1,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution for 1 hour at room temperature.

    Lane 1: Phospho-GSK3 alpha (S21) peptide (positive)
    Lane 2: Unmodified GSK3 alpha peptide (negative)
    Lane 3: Phospho-GSK3 beta (S9) peptide (negative)
    Lane 4: Unmodified GSK3 beta peptide (negative)

    Proteins loading: 100ng, 25ng, 5ng;

    Blocking and dilution buffer: 5% NFDM/TBST;

    Exposure time: 10 seconds; ECL: K1801.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

同靶点 & 同通路的产品

metafield image

Phospho-GSK3 alpha (S21) Recombinant Rabbit Monoclonal Antibody [PSH09-11] - BSA and Azide free

Application: WB,IHC-P,Dot Blot

Reactivity: Human,Mouse

Conjugate: unconjugated

metafield image

GSK3 alpha Recombinant Rabbit Monoclonal Antibody [JE33-27]

Application: WB,IHC-P

Reactivity: Human,Mouse,Rat,Monkey

Conjugate: unconjugated

metafield image

GSK3 alpha Rabbit Polyclonal Antibody

Application: WB,IF-Cell,IHC-P,FC

Reactivity: Human

Conjugate: unconjugated

Close (esc)

Popup

Use this popup to embed a mailing list sign up form. Alternatively use it as a simple call to action with a link to a product or a page.

Age verification

By clicking enter you are verifying that you are old enough to consume alcohol.

Enter

搜索

主菜单