Human TNF-alpha, C-His Tag
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Product Information
Product name
Human TNF-alpha, C-His Tag
Catalog No.
HA210761
Predicted molecular mass
18.7 kD
Protein construction description
A DNA sequence encoding the human TNF-alpha protein (P01375) (Val 77-Leu 233) was expressed with a His tag at the C-terminus.
Accession
P01375
Protein construction
Source
HEK293
Bio Activity
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25-100 pg/mL.
Purity
>95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Formulation
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
Species
Human
Background
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective. Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistanc.
Usage guide
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped with blue ice. Bulk packages of recombinant proteins are provided as frozen liquid which are shipped with dry ice.
Storage
Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
Reconstitution
Reconstitute at 250 μg/ml in sterile water.
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Human VE-cadherin, Tag Free (ECD)
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Human IL-8/CXCL8 Tag Free
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Human EPCR/PROCR/CD201, C-His, Flag Tag (ECD)
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Human CDCP1/CD318, N-Twin Strep, C-His, Flag Tag
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Human CD200, C-His Tag (ECD)
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Human IL-18 BPa, C-His Tag
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Conjugate:
Human IL-22, C-His, Flag Tag
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Reactivity: Human
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Human IL1RL1/DER4/ST2, C-His (ECD)
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Reactivity: Human
Conjugate:
Human IL-17F, Tag Free
Application:
Reactivity: Human
Conjugate:
Human EGF, Tag Free
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Reactivity: Human
Conjugate:
Human LILRB4/ILT3, C-His, Flag Tag (ECD)
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Reactivity: Human
Conjugate:
Human IL-4R, C-His, Tag (ECD)
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Reactivity: Human
Conjugate:
Human IL-10, C-His Tag
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Reactivity: Human
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Human IFNA1/IFNA13, C-Tag
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Reactivity: Human
Conjugate:
Human CXCL10, C-Flag Tag
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Reactivity: Human
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Human LTA, Tag Free
Application:
Reactivity: Human
Conjugate:
Human NRG1, C-Flag (176-246)
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Reactivity: Human
Conjugate:
Human RAC1, C-Flag Tag
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Reactivity: Human
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Human CDC42, Tag Free
Application:
Reactivity: Human
Conjugate:
Human NRG1, Tag Free
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Reactivity: Human
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Human IL-15, C-Flag Tag
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Reactivity: Human
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Human CD207, C-Flag Tag
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Reactivity: Human
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Human ATF6, Tag Free
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Reactivity: Human
Conjugate:
Human NCAM1/CD56, Tag Free (ECD)
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Reactivity: Human
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Human CD5, Tag Free (ECD)
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Reactivity: Human
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Human IL1RA, C-His Tag
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Reactivity: Human
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Human CXCL1, Tag Free
Application:
Reactivity: Human
Conjugate:
Human TIE2/TEK, C-His, Flag Tag (ECD)
Application:
Reactivity: Human
Conjugate:
Human HVEM/TNFRSF14, Tag Free (ECD)
Application:
Reactivity: Human
Conjugate:
Human MMP-3, C-His, Flag Tag
Application:
Reactivity: Human
Conjugate:
Human CTLA-4, C-His Tag
Application:
Reactivity: Human
Conjugate:
Human CXCL5, Tag Free
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Reactivity: Human
Conjugate:
Human CD31/PECAM-1, C-His Tag
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Reactivity: Human
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Human TRAIL/TNFSF10, C-His Tag
Application:
Reactivity: Human
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Human IP-10/CXCL10, Tag Free
Application:
Reactivity: Human
Conjugate:
Human CCL3/MIP-1, Tag Free
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Reactivity: Human
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Human MMP-1, C-Flag
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Reactivity: Human
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Human CXCL2/GRO beta, Tag Free
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Reactivity: Human
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Human IL-13, Tag Free
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Reactivity: Human
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Human IL-9, C-Flag Tag
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Reactivity: Human
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Human CD28, C-His Tag
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Reactivity: Human
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Human PD-1, C-His Tag
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Reactivity: Human
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Human IL-3, Tag Free
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Reactivity: Human
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Human CD58/LFA-3, C-His Tag (ECD)
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Reactivity: Human
Conjugate:
Human CXCL9, Tag Free
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Reactivity: Human
Conjugate:
Human CEACAM-1/CD66a, C-His Tag (ECD)
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Reactivity: Human
Conjugate:
Human TNF RI/TNFRSF1A, Tag Free (ECD)
Application:
Reactivity: Human
Conjugate:
Human IL-1 beta/IL1B, C-His Tag
Application:
Reactivity: Human
Conjugate:
Human IL17RA/CD217, C-His Tag (ECD)
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Reactivity: Human
Conjugate:
Human MAPK6, C-His Tag
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Reactivity: Human
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Human ICOS Ligand/B7-H2/ICOSLG, C-His Tag (ECD)
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Reactivity: Human
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Human IL-7, C-His, Flag Tag
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Reactivity: Human
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Human TRAF3, C-His Tag
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Reactivity: Human
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Human IFN-alpha/beta R2, C-His Tag (ECD)
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Reactivity: Human
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Human cIAP-2/BIRC3, N-Twin Strep, C-His, Flag Tag
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Reactivity: Human
Conjugate:
Human IFN-alpha/beta R1, C-His (ECD)
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Reactivity: Human
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Human IL-6, C-His Tag
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Reactivity: Human
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Human LILRB2/CD85d/ILT4, C-His, Flag Tag (ECD)
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Reactivity: Human
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Human CD30/TNFRSF8, C-His, Flag Tag (ECD)
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Reactivity: Human
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Human IL12B/P40, C-His Tag
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Reactivity: Human
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Human PPID, C-His Tag
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Reactivity: Human
Conjugate:
Human KIF5B, N-Twin Strep, C-His Tag
Application:
Reactivity: Human
Conjugate:
Human IL-1 RII, C-His Tag (ECD)
Application:
Reactivity: Human
Conjugate:
Human CYLD, C-His Tag
Application:
Reactivity: Human
Conjugate:
Human GM-CSF, Tag Free
Application:
Reactivity: Human
Conjugate:
Human LILRB1/CD85j/ILT2, C-His, Flag Tag (ECD)
Application:
Reactivity: Human
Conjugate:
Human Noggin/NOG, Tag Free
Application:
Reactivity: Human
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Human IL5RA, C-His Tag (ECD)
Application:
Reactivity: Human
Conjugate:
Human IL12RB1, C-His Tag (ECD)
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Reactivity: Human
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Human GADD45A, N-Twin Strep, C-His, Flag Tag
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Reactivity: Human
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Human CCL3L1, N-Twin Strep, C-His, Flag Tag
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Reactivity: Human
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Human TNF-alpha, Tag Free
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Reactivity: Human
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Human IFN-gamma, C-His Tag
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Reactivity: Human
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Human IL-4, Tag Free
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Human IL-2R alpha, Tag Free
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Human IL-18, Tag Free
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Human DVL2, C Twin Strep, Flag
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Human VEGF165, Tag Free
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Human CCL2/MCP1, Tag Free
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Human IL-2, Tag Free
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Human GADD45B, N-Twin Strep, C-His, Flag Tag
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Reactivity: Human
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Human IL-6, Tag Free
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Human CD27/TNFRSF7, Tag Free
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Reactivity: Human
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Human ANPEP/CD13, Tag Free (ECD)
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Reactivity: Human
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Human OX40/TNFRSF4, C-His Tag
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Reactivity: Human
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Human EIF4B, C-His Tag
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Reactivity: Human
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Human 4-1BB/TNFRSF9, C-His Tag
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Human TREM1, C-Hia, Flag Tag
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Human CCL11/Eotaxin, Tag Free
Application:
Reactivity: Human
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Human PD-L1/B7-H1, C-hFc Tag
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Reactivity: Human
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Human BAFF/BLyS/TNFSF13B, Tag Free (ECD)
Application:
Reactivity: Human
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Human CD48, C-His Tag
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Reactivity: Human
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Human 5'-nucleotidase/CD73/NT5E, C-His Tag
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Reactivity: Human
Conjugate:
Human IL-16, C-His Tag
Application:
Reactivity: Human
Conjugate:
Human M-CSF/CSF1 (33-255), C His Tag
Application:
Reactivity: Human
Conjugate:
Human IL-31, C-Flag Tag
Application:
Reactivity: Human
Conjugate:
Human MAPK12, C-His Tag
Application:
Reactivity: Human
Conjugate:
Human IFN-gamma R1, C-His Tag (ECD)
Application:
Reactivity: Human
Conjugate:
Human CSK, C-His Tag
Application:
Reactivity: Human
Conjugate:
Human COX5A, C-His Tag
Application:
Reactivity: Human
Conjugate:
Human IL-2RB/CD122, C-His Tag (ECD)
Application:
Reactivity: Human
Conjugate:
Human CD2, C-His, Flag Tag (ECD)
Application:
Reactivity: Human
Conjugate:
Human TGF-beta RII, Tag Free (ECD)
Application:
Reactivity: Human
Conjugate:
Human IL-5, C-His Tag
Application:
Reactivity: Human
Conjugate:
Human HCLS1, N-Strep II, C-His Tag
Application:
Reactivity: Human
Conjugate:
Human IL-17A, Tag Free
Application:
Reactivity: Human
Conjugate:
Human CD63, Tag Free (ECD)
Application:
Reactivity: Human
Conjugate:
Human CD2, N-Twin Strep, C-His, Flag Tag
Application:
Reactivity: Human
Conjugate:
Human CD272/BTLA, C-His, Flag Tag (ECD)
Application:
Reactivity: Human
Conjugate:
Human LAIR1, N-Twin Strep, C-His, Flag Tag
Application:
Reactivity: Human
Conjugate:
Human IL-21, C-His, Flag Tag
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Reactivity: Human
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Human IL-7, Tag Free
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Reactivity: Human
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Human IGF1, Tag Free
Application:
Reactivity: Human
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Human Angiopoietin-1/ANGPT1, Tag Free
Application:
Reactivity: Human
Conjugate:
Human CCL17/TARC, Tag Free
Application:
Reactivity: Human
Conjugate:
Human CXCL12/SDF-1, Tag Free
Application:
Reactivity: Human
Conjugate:
Human Nectin-2, C-His Tag (ECD)
Application:
Reactivity: Human
Conjugate:
Human M-CSF/CSF1 (33-190), Tag Free
Application:
Reactivity: Human
Conjugate:
Human VEGF121, Tag Free
Application:
Reactivity: Human
Conjugate:
Human P-Selectin/CD62P, C-His Tag (ECD)
Application:
Reactivity: Human
Conjugate:
Human PRDM1, N-Twin Strep, C-His, Flag Tag
Application:
Reactivity: Human
Conjugate:
Human E-Cadherin, C-His Tag (ECD)
Application:
Reactivity: Human
Conjugate:
Human EIF2AK3/PERK, C-His Tag
Application:
Reactivity: Human
Conjugate:
Human HVEM/TNFRSF14, C-Flag Tag
Application:
Reactivity: Human
Conjugate:
Human Interferon alpha-2/IFNA2, C-His Tag
Application:
Reactivity: Human
Conjugate:
Human IL-2, C-His Tag
Application:
Reactivity: Human
Conjugate:
Human G-CSF, C-His Tag
Application:
Reactivity: Human
Conjugate:
Human ICAM-1/CD54, C-His Tag (ECD)
Application:
Reactivity: Human
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