Human IGF1, Tag Free
Catalog# HA210866
Human IGF1, Tag Free
Product Information
Product name
Human IGF1, Tag Free
Catalog No.
HA210866
Predicted molecular mass
8 kD
Protein construction description
A DNA sequence encoding the human IGF1 protein (P05019-1) (Gly 49-Ala 118) was expressed with tag free.
Accession
P05019-1
Protein construction
Source
HEK293
Bio Activity
Testing in progress.
Purity
>95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Formulation
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
Species
Human
Background
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca2+-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1.
Usage guide
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped with blue ice. Bulk packages of recombinant proteins are provided as frozen liquid which are shipped with dry ice.
Storage
Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
Reconstitution
Reconstitute at 250 μg/ml in sterile water.
同靶点&同通路的产品
Human G-CSF, C-His Tag
Application:
Reactivity: Human
Conjugate:
Human FGF7, Tag Free
Application:
Reactivity: Human
Conjugate:
Human HGF, Tag Free
Application:
Reactivity: Human
Conjugate:
Human IL-2, C-His Tag
Application:
Reactivity: Human
Conjugate:
Human IFN-gamma R1, C-His Tag (ECD)
Application:
Reactivity: Human
Conjugate:
Human IL2RG/CD132, C-His Tag (ECD)
Application:
Reactivity: Human
Conjugate:
Human Interferon alpha-2/IFNA2, C-His Tag
Application:
Reactivity: Human
Conjugate:
Human Prolactin/PRL, C-His, Flag Tag
Application:
Reactivity: Human
Conjugate:
Human IL-4R, C-His, Tag (ECD)
Application:
Reactivity: Human
Conjugate:
Human TNF-alpha, Tag Free
Application:
Reactivity: Human
Conjugate:
Human TNF-alpha, C-His Tag
Application:
Reactivity: Human
Conjugate:
Human FGF7, C-Flag Tag
Application:
Reactivity: Human
Conjugate:
Human VEGF165, Tag Free
Application:
Reactivity: Human
Conjugate:
Human FGF Basic/FGF2, Tag Free
Application:
Reactivity: Human
Conjugate:
Human HGF, C-Flag Tag
Application:
Reactivity: Human
Conjugate:
Human IL-2R alpha, Tag Free
Application:
Reactivity: Human
Conjugate:
Human IL-2RB/CD122, C-His Tag (ECD)
Application:
Reactivity: Human
Conjugate:
Human IL-7, C-His, Flag Tag
Application:
Reactivity: Human
Conjugate:
Human IFN-gamma, C-His Tag
Application:
Reactivity: Human
Conjugate:
Human FGF10, C-Flag
Application:
Reactivity: Human
Conjugate:
Human EIF4B, C-His Tag
Application:
Reactivity: Human
Conjugate:
Human GADD45B, N-Twin Strep, C-His, Flag Tag
Application:
Reactivity: Human
Conjugate:
Human TNF RI/TNFRSF1A, Tag Free (ECD)
Application:
Reactivity: Human
Conjugate:
Human FGF2/b-FGF, C-His, Flag Tag
Application:
Reactivity: Human
Conjugate:
Human IGFBP3/IBP3, C-His, Flag Tag
Application:
Reactivity: Human
Conjugate:
Human IFN-alpha/beta R2, C-His Tag (ECD)
Application:
Reactivity: Human
Conjugate:
Human SCF/KITLG, C-His Tag (ECD)
Application:
Reactivity: Human
Conjugate:
Human IFN-alpha/beta R1, C-His (ECD)
Application:
Reactivity: Human
Conjugate:
Human IL-6, C-His Tag
Application:
Reactivity: Human
Conjugate:
Human IL-6R, C-His (ECD)
Application:
Reactivity: Human
Conjugate:
Human EGF, Tag Free
Application:
Reactivity: Human
Conjugate:
Human IFNA1/IFNA13, C-Tag
Application:
Reactivity: Human
Conjugate:
Human GADD45A, N-Twin Strep, C-His, Flag Tag
Application:
Reactivity: Human
Conjugate:
Human RAC1, C-Flag Tag
Application:
Reactivity: Human
Conjugate:
Human IL-4, Tag Free
Application:
Reactivity: Human
Conjugate:
Human DVL2, C Twin Strep, Flag
Application:
Reactivity: Human
Conjugate:
Human IL-2, Tag Free
Application:
Reactivity: Human
Conjugate:
Human IL-6, Tag Free
Application:
Reactivity: Human
Conjugate:
Human IL-7, Tag Free
Application:
Reactivity: Human
Conjugate:
Human TIE2/TEK, C-His, Flag Tag (ECD)
Application:
Reactivity: Human
Conjugate:
Human Erythropoietin/EPO, C-His, Flag Tag
Application:
Reactivity: Human
Conjugate:
Human PDGF-B, Tag Free
Application:
Reactivity: Human
Conjugate:
Human VEGF-C, C-His, Flag Tag
Application:
Reactivity: Human
Conjugate:
Human Angiopoietin-1/ANGPT1, Tag Free
Application:
Reactivity: Human
Conjugate:
Human M-CSF/CSF1 (33-255), C His Tag
Application:
Reactivity: Human
Conjugate:
Human M-CSF/CSF1 (33-190), Tag Free
Application:
Reactivity: Human
Conjugate:
Human FGF-21, C-His Tag
Application:
Reactivity: Human
Conjugate:
Human IL-3, Tag Free
Application:
Reactivity: Human
Conjugate:
