mWasabi Nanoselector Magnetic beads

产品描述

Description:
mWassabi Nanoselector Magnetic beads have been specifically designed to bind mWassabi-fusion proteins. mWassabi Nanoselector Magnetic beads are based on small high-affinity recombinant single domain antibody covalently coupled to the surface of Magnetic beads. mWassabi Nanoselector Magnetic beads are ideal tools to isolate or purify mWassabi-fusion proteins fast and efficiently.

Ligand: Anti-mWassabi single domain antibody fragment (VHH, Nanobody)
Bead size: ~ 40µm
Reactivity: Highly selective for mWassabi fusion protein
Binding Capacity: High binding capacity, 10 µL slurry bind about 20 µg of recombinant mWassabi protein.
Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.
Storage Buffer: 10mM PBS (pH 7.5), 0.01% proclin300

Background:
Fluorescent proteins are widely used to study protein localization and dynamics. mWasabi is engineered variant of monomeric mTFP1, and it is a bright, monomeric green fluorescent protein. mWasabi is nearly 2-fold brighter than EGFP. And different from many commonly used fluorescent proteins, mWasabi is a true monomer and is most likely to not interfere with function or localization of its fusion partner. So mWasabi can be a direct replacement for EGFP or other GFPs. mWassabi-fusion proteins and their interacting factors can to be isolated fast and efficiently by immunoprecipitation using the mWassabi Nanoselector Magnetic beads. Due to the single-chain nature of sdAbs and their stable and covalent attachment, no leakage of light and heavy chains is observed during elution with SDS sample buffer.

产品性能

Fluorescent proteins are widely used to study protein localization and dynamics. mWasabi is engineered variant of monomeric mTFP1, and it is a bright, monomeric green fluorescent protein. mWasabi is nearly 2-fold brighter than EGFP. And different from many commonly used fluorescent proteins, mWasabi is a true monomer and is most likely to not interfere with function or localization of its fusion partner. So mWasabi can be a direct replacement for EGFP or other GFPs. mWassabi-fusion proteins and their interacting factors can to be isolated fast and efficiently by immunoprecipitation using the mWassabi Nanoselector Magnetic beads. Due to the single-chain nature of sdAbs and their stable and covalent attachment, no leakage of light and heavy chains is observed during elution with SDS sample buffer.

存放说明

Shipped at ambient temperature. Upon receipt store at 4℃. Stable for 1 year. Do not freeze.