Strep-tag II Nanoselector Magnetic beads

产品描述

Description:
Strep-tag II Nanoselector Magnetic beads have been specifically designed to bind Strep-tag II-fusion proteins. Strep-tag II Nanoselector Magnetic beads are based on small high-affinity recombinant alpaca antibody fragments covalently coupled to the surface of magnetic beads. Strep-tag II Nanoselector Magnetic beads are an ideal tool to isolate or purify Strep-tag II-fusion proteins fast and efficiently.


Ligand: Anti-Strep-tag II single domain antibody fragment (VHH, Nanobody)
Bead size: ~ 40 µm
Reactivity: Recognizes Strep-tag II fusion protein selectively
Binding Capacity: High binding capacity, 10 µL slurry bind about 10 µg of recombinant Strep-tag II-GFP.
Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.
Storage Buffer: 10mM PBS (pH 7.5), 0.01% proclin300

Background:
Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag(AWRHPQFGG) was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II(WSHPQFEK).

产品性能

Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag(AWRHPQFGG) was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II(WSHPQFEK).

存放说明

Shipped at ambient temperature. Upon receipt store at 4℃. Stable for 1 year. Do not freeze.