TagFP Nanoselector Magnetic beads

产品描述

Description:
TagFP Nanoselector Magnetic beads have been specifically designed to bind TagFP-fusion proteins. TagFP Nanoselector Magnetic beads are based on small high-affinity recombinant single domain antibody covalently coupled to the surface of Magnetic beads. TagFP Nanoselector Magnetic beads are ideal tools to isolate or purify TagFP-fusion proteins fast and efficiently.

Ligand: Anti-TagRFP/TagBFP single domain antibody fragment (VHH, Nanobody)
Bead size: ~ 40µm
Reactivity: Highly selective for TagRFP/TagBFP. Does not cross-react with common GFP or dsRed derivatives.
Binding Capacity: High binding capacity, 10 µL slurry bind about 20 µg of recombinant TagRFP/TagBFP protein.
Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.
Storage Buffer: 10mM PBS (pH 7.5), 0.01% proclin300

Background:
TagRFP is derived from the Entacmaea quadricolor fluorescent protein TurboRFP (a random mutant of eqFP578), with mutations of R162E, Q166D, S180N, F198V, F200Y at the hydrophilic interface. TagBFP was derived from TagRFP with the some mutations. TagRFP/TagBFP has a high fluorescent quantum yield (Φfluo 0.48) and is widely used for fluorescent imaging. TagFP-fusion proteins and their interacting factors can to be isolated fast and efficiently by immunoprecipitation using the TagFP Nanoselector Magnetic beads. Due to the single-chain nature of sdAbs and their stable and covalent attachment, no leakage of light and heavy chains is observed during elution with SDS sample buffer.

产品性能

TagRFP is derived from the Entacmaea quadricolor fluorescent protein TurboRFP (a random mutant of eqFP578), with mutations of R162E, Q166D, S180N, F198V, F200Y at the hydrophilic interface. TagBFP was derived from TagRFP with the some mutations. TagRFP/TagBFP has a high fluorescent quantum yield (Φfluo 0.48) and is widely used for fluorescent imaging. TagFP-fusion proteins and their interacting factors can to be isolated fast and efficiently by immunoprecipitation using the TagFP Nanoselector Magnetic beads. Due to the single-chain nature of sdAbs and their stable and covalent attachment, no leakage of light and heavy chains is observed during elution with SDS sample buffer.

存放说明

Shipped at ambient temperature. Upon receipt store at 4℃. Stable for 1 year. Do not freeze.