Spycatcher003 Nanoselector Magnetic beads

产品描述

Description:
SpyCatcher003 Magnetic beads have been specifically designed to bind SpyTag003-fusion proteins. SpyCatcher003 Magnetic beads are based on Spycatcher003 covalently coupled to the surface of magnetic beads, and they are used to detect and isolate SpyTag003-fusion proteins efficiently.

Ligand: SpyCatcher003
Bead size: ~ 40 µm
Reactivity: Highly selective for SpyTag003-fusion proteins.
Binding Capacity: High binding capacity, 10 µL slurry bind about 10-20µg of SpyTag003-fusion protein.
Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.
Storage Buffer: 10mM PBS (pH 7.5), 0.01% proclin300.

Background:
The SpyCatcher-SpyTag system was developed seven years ago as a method for protein ligation. It is based on a modified domain from a Streptococcus pyogenes surface protein (SpyCatcher), which recognizes a cognate 13-amino-acid peptide (SpyTag). Upon recognition, the two form a covalent isopeptide bond between the side chains of a lysine in SpyCatcher and an aspartate in SpyTag.  This technology has been used, among other applications, to create covalently stabilized multi-protein complexes, for modular vaccine production, and to label proteins. The SpyTag system is versatile as the tag is a short, unfolded peptide that can be genetically fused to exposed positions in target proteins; similarly, SpyCatcher can be fused to reporter proteins such as GFP, and to epitope or purification tags.

SpyCatcher003-SpyTag003 system  is a variant of SpyCatcher-SpyTag system, the 003 mutations is able to improve both the noncovalent association rate and the rate of reaction following this noncovalent docking.

产品性能

The SpyCatcher-SpyTag system was developed seven years ago as a method for protein ligation. It is based on a modified domain from a Streptococcus pyogenes surface protein (SpyCatcher), which recognizes a cognate 13-amino-acid peptide (SpyTag). Upon recognition, the two form a covalent isopeptide bond between the side chains of a lysine in SpyCatcher and an aspartate in SpyTag.  This technology has been used, among other applications, to create covalently stabilized multi-protein complexes, for modular vaccine production, and to label proteins. The SpyTag system is versatile as the tag is a short, unfolded peptide that can be genetically fused to exposed positions in target proteins; similarly, SpyCatcher can be fused to reporter proteins such as GFP, and to epitope or purification tags. SpyCatcher003-SpyTag003 system  is a variant of SpyCatcher-SpyTag system, the 003 mutations is able to improve both the noncovalent association rate and the rate of reaction following this noncovalent docking.

存放说明

Shipped at ambient temperature. Upon receipt store at 4℃. Stable for 1 year. Do not freeze.