Mouse Resistin enzyme-linked immunoassay kit(one step)
Rmb: 4500
Catalog# EMY0063
Mouse Resistin enzyme-linked immunoassay kit(one step)
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ELISA
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Mouse
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1.56~100 ng/ml
概述
产品名称
Mouse Resistin enzyme-linked immunoassay kit(one step)
产品类型
Elisa Kit
灵敏度
0.001 ng/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0286 | 0.0296 | 0.0291 | |
| 1.56 | 0.0844 | 0.0949 | 0.0897 | 0.0606 |
| 3.13 | 0.1841 | 0.1639 | 0.1740 | 0.1449 |
| 6.25 | 0.3404 | 0.3503 | 0.3454 | 0.3163 |
| 12.50 | 0.6524 | 0.5893 | 0.6209 | 0.5918 |
| 25.00 | 1.1861 | 1.1035 | 1.1448 | 1.1157 |
| 50.00 | 2.3761 | 2.4024 | 2.3893 | 2.3602 |
| 100.00 | 3.9695 | 4.1788 | 4.0742 | 4.0451 |
精度
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 1.9 | 10.5 | 28.4 | 1.9 | 9.3 | 28.6 |
| Standard Deviation | 0.1 | 0.7 | 1.6 | 0.1 | 0.6 | 1.0 |
| Coefficient of Variation (%) | 6.1 | 6.4 | 5.8 | 5.5 | 6.0 | 3.5 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
加标回收
健康小鼠血清加入 3 个不同浓度水平的小鼠Resistin，未加小鼠的Resistin血清作为本底，计算回收率。回收率的范围从 88 % 至 105%，平均回收率为 97%。
样本值
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 5.23-18.70 | 100 | 11.5 |
应用本试剂盒，检测 30 个健康小鼠的血清样本，小鼠的用药史不详。
n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
实验类型
种属反应性
检测方法
操作时长
标准曲线梯度
孵育次数
2
可检测样本
加样体积
产品特性
储存条件
存储于 2-8 ℃
靶点
UNIPROT
别名
Adipose tissue specific secretory factor
Adipose tissue-specific secretory factor
ADSF
C/EBP epsilon regulated myeloid specific secreted cysteine rich protein
C/EBP epsilon regulated myeloid specific secreted cysteine rich protein precursor 1
C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein
CG5403
Cysteine rich secreted protein A12 alpha like 2
Cysteine rich secreted protein FIZZ3
Cysteine-rich secreted protein A12-alpha-like 2
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