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CD11b Mouse Monoclonal Antibody [A2-9]

RMB: 1500

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Catalog# M1211-6

CD11b Mouse Monoclonal Antibody [A2-9]

Application

  • WB

  • IHC-P

  • FC

  • IF-Tissue

Reactivity

  • Human

Conjugation

  • unconjugated

概述

产品名称

CD11b Mouse Monoclonal Antibody [A2-9]

抗体类型

Mouse Monoclonal Antibody

免疫原

Recombinant protein within human CD11b 500-750 aa.

种属反应性

Human

验证应用

WB, IHC-P, FC, IF-Tissue

分子量

Predicted band size: 127 kDa

阳性对照

TF-1 cell lysate, human tonsil tissue, human spleen tissue, Raji.

偶联

unconjugated

克隆号

A2-9

RRID

AB_3073051

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG2b

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

  • FC

  • 1:200

  • IF-Tissue

  • 1:200

靶点

功能

Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.

背景文献

1. "JAM-C is a component of desmosomes and a ligand for CD11b/CD18-mediated neutrophil transepithelial migration." Zen K., Babbin B.A., Liu Y., Whelan J.B., Nusrat A., Parkos C.A. Mol. Biol. Cell 15:3926-3937(2004)

2. Nath S.K., Han S "A nonsynonymous functional variant in integrin-alpha(M) (encoded by ITGAM) is associated with systemic lupus erythematosus."., Kim-Howard X., Kelly J.A., Viswanathan P., Gilkeson G.S., Chen W., Zhu C., McEver R.P., Kimberly R.P., Alarcon-Riquelme M.E., Vyse T.J., Li Q.-Z., Wakeland E.K., Merrill J.T., James J.A., Kaufman K.M., Guthridge J.M., Harley J.B. Nat. Genet. 40:152-154(2008)

序列相似性

Belongs to the integrin alpha chain family.

组织特异性

Predominantly expressed in monocytes and granulocytes. Expressed in neutrophils (at protein level).

亚细胞定位

Membrane.

UNIPROT

SwissProt: P11215 Human

别名

antigen CD11b (p170) antibody

Antigen CD11b p170 antibody

CD11 antigen like family member B antibody

CD11 antigen-like family member B antibody

CD11b antibody

CD11b/CD18 antibody

CD49d antibody

Cell surface glycoprotein MAC-1 subunit alpha antibody

Complement component 3 receptor 3 subunit antibody

Complement Component Receptor 3 Alpha antibody

展开

图片

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of CD11b on different lysates with Mouse anti-CD11b antibody (<a href="/products/M1211-6" style="font-weight: bold;text-decoration: underline;">M1211-6</a>) at 1/1,000 dilution.<br /><br />Lane 1: TF-1 cell lysate<br />Lane 2: Jurkat cell lysate (negative)<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 127 kDa<br />Observed band size: 170 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/M1211-6" style="font-weight: bold;text-decoration: underline;">M1211-6</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of CD11b on different lysates with Mouse anti-CD11b antibody (M1211-6) at 1/1,000 dilution.

    Lane 1: TF-1 cell lysate
    Lane 2: Jurkat cell lysate (negative)

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 127 kDa
    Observed band size: 170 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1211-6) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD11b antibody (<a href="/products/M1211-6" style="font-weight: bold;text-decoration: underline;">M1211-6</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M1211-6" style="font-weight: bold;text-decoration: underline;">M1211-6</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD11b antibody (M1211-6) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1211-6) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-CD11b antibody (<a href="/products/M1211-6" style="font-weight: bold;text-decoration: underline;">M1211-6</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M1211-6" style="font-weight: bold;text-decoration: underline;">M1211-6</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-CD11b antibody (M1211-6) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1211-6) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: IF-Tissue<br /><br />Species: Human<br /><br />Site: Spleen<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/200

    Application: IF-Tissue

    Species: Human

    Site: Spleen

    Sample: Paraffin-embedded section

    Antibody concentration: 1/200

  • Flow cytometric analysis of Raji cells with CD11b antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.

    Flow cytometric analysis of Raji cells with CD11b antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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