CD11b Mouse Monoclonal Antibody [4-6]
Rmb: 1500
Catalog# EM1701-42
CD11b Mouse Monoclonal Antibody [4-6]
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IHC-P
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IF-Cell
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FC
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WB
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IF-Tissue
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Human
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unconjugated
概述
产品名称
CD11b Mouse Monoclonal Antibody [4-6]
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant protein within Human CD11b aa 544-743 / 1,152.
种属反应性
Human
验证应用
IHC-P, IF-Cell, FC, WB, IF-Tissue
靶点分子量
Predicted band size: 127 kDa
阳性对照
TF-1 cell lysate, THP-1 cell lysate, U-937 cell lysate, A431, LOVO, human tonsil tissue, human spleen tissue.
偶联
unconjugated
克隆号
4-6
RRID
反应性数据
已验证（内部验证通过）
文献已报道（未内部验证，但有文献支持）
预测可反应（基于高同源性）
不推荐（内部验证未通过）
| WB | IF-Tissue | |
|---|---|---|
| human |
|
|
| mouse |
|
|
| rat |
|
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG2b
纯化方式
Protein G affinity purified.
应用稀释度
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
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WB
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1:1,000
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IF-Tissue
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1:50
靶点
功能
Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration. In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils. May regulate phagocytosis-induced apoptosis in extravasated neutrophils. May play a role in mast cell development.
背景文献
1. Jerke U et al.Complement receptor Mac-1 is an adaptor for NB1 (CD177)-mediated PR3-ANCA neutrophil activation. J. Biol. Chem. 286:7070-7081 (2011).
2. Bai M. et al. CD177 modulates human neutrophil migration through activation-mediated integrin and chemoreceptor regulation. Blood 0:0-0 (2017).
序列相似性
Belongs to the integrin alpha chain family.
组织特异性
Predominantly expressed in monocytes and granulocytes. Expressed in neutrophils (at protein level).
亚细胞定位
Cell membrane.
UNIPROT
别名
antigen CD11b (p170) antibody
Antigen CD11b p170 antibody
CD11 antigen like family member B antibody
CD11 antigen-like family member B antibody
CD11b antibody
CD11b/CD18 antibody
CD49d antibody
Cell surface glycoprotein MAC-1 subunit alpha antibody
Complement component 3 receptor 3 subunit antibody
Complement Component Receptor 3 Alpha antibody
展开图片
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Western blot analysis of CD11b on different lysates with Mouse anti-CD11b antibody (EM1701-42) at 1/1,000 dilution.
Lane 1: TF-1 cell lysate
Lane 2: THP-1 cell lysate
Lane 3: U-937 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 127 kDa
Observed band size: 170 kDa
Exposure time: 30 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-42) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
ICC staining CD11b (green) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining CD11b (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD11b antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD11b antibody. Counter stained with hematoxylin.
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Application: IF-Tissue
Species: Human
Site: Spleen
Sample: Paraffin-embedded section
Antibody concentration: 1/50
引文
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Optimized CAR-T therapy based on spatiotemporal changes and chemotactic mechanisms of MDSCs induced by hypofractionated radiotherapy
期刊: Molecular Therapy
DOI:
IF: 12.4
应用: FC
反应种属: Mouse
发表时间: 2023 Jul
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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