BRN3A Recombinant Rabbit Monoclonal Antibody [PSH11-65]
Rmb: 699 特惠 1500
Catalog# HA723361
BRN3A Recombinant Rabbit Monoclonal Antibody [PSH11-65]
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IHC-Fr
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IHC-P
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WB
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mIHC
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Mouse
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Rat
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HA751421
不含抗保成分
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unconjugated
概述
产品名称
BRN3A Recombinant Rabbit Monoclonal Antibody [PSH11-65]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Mouse BRN3A aa 1-421.
种属反应性
Mouse, Rat
验证应用
IHC-Fr, IHC-P, WB, mIHC
靶点分子量
Predicted band size: 43 kDa
阳性对照
Mouse embryonic brain tissue, mouse embryonic cochlear vestibular ganglion tissue, E14.5 mouse embryonic eye tissue, E14.5 rat embryo tissue, E14.0 mouse embryonic brain tissue lysates.
偶联
unconjugated
克隆号
PSH11-65
反应性数据
已验证（内部验证通过）
文献已报道（未内部验证，但有文献支持）
预测可反应（基于高同源性）
不推荐（内部验证未通过）
| WB | IHC-P | IHC-Fr | mIHC | |
|---|---|---|---|---|
| mouse |
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| rat |
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产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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IHC-Fr
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1:500
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IHC-P
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1:50-1:200
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WB
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1:1,000
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mIHC
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1:200-1:500
靶点
功能
POU domain, class 4, transcription factor 1 (POU4F1) also known as brain-specific homeobox/POU domain protein 3A (BRN3A), homeobox/POU domain protein RDC-1 or Oct-T1 is a protein that in humans is encoded by the POU4F1 gene. BRN3A (POU4F1) is a class IV POU domain-containing transcription factor highly expressed in the developing peripheral sensory nervous system (dorsal root ganglia, trigeminal ganglion, and hindbrain sensory ganglia), certain regions of the central nervous system, retinal neurons called ganglion cells, and in cells of the B- and T-lymphocytic lineages.
背景文献
1. Nishida K et al. Brn3a controls the soma localization and axonal extension patterns of developing spinal dorsal horn neurons. PLoS One. 2023 Sep
2. Heppt MV et al. HDAC2 Is Involved in the Regulation of BRN3A in Melanocytes and Melanoma. Int J Mol Sci. 2022 Jan
亚细胞定位
Nucleus, Cytoplasm.
别名
Brain specific homeobox/POU domain protein 3A antibody
Brain-3A antibody
Brain-specific homeobox/POU domain protein 3A antibody
Brn 3.0 antibody
BRN 3A antibody
Brn-3A antibody
Brn3 antibody
BRN3A antibody
class 4 antibody
FLJ13449 antibody
展开图片
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mIHC analysis of mouse retina tissue (Frozen section) with BRN3A (HA723361, 1/500) and βIII tubulin (HA723738, 1/5,000).
The immunostaining was performed with the IRISKitCmTSA Kit (900802). Blocking with 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner. -
Application: Immunofluorescence (IHC-Fr)
Species: Mouse
Tissue: Retina
Sample: Frozen section
Antigen retrieval: Not required
Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA723361, 1/500, overnight at 4℃.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. -
Application: Immunofluorescence (IHC-Fr)
Species: Mouse
Tissue: E14.5 embryonic brain
Sample: Frozen section
Antigen retrieval: Not required
Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA723361, 1/500, overnight at 4℃.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. -
Application: Immunofluorescence (IHC-Fr)
Species: Mouse
Tissue: E14.5 embryonic cochlear vestibular ganglion
Sample: Frozen section
Antigen retrieval: Not required
Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA723361, 1/500, overnight at 4℃.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. -
Immunohistochemical analysis of paraffin-embedded E14.5 mouse embryonic eye tissue with Rabbit anti-BRN3A antibody (HA723361) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723361) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded E14.5 rat embryo tissue with Rabbit anti-BRN3A antibody (HA723361) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723361) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Western blot analysis of BRN3A on E14.0 mouse embryonic brain tissue lysates with Rabbit anti-BRN3A antibody (HA723361) at 1/1,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 43 kDa
Observed band size: 47 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723361) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
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