CD11b Recombinant Rabbit Monoclonal Antibody [JE01-17]
Rmb: 950 特惠 1500
Catalog# HA722856
CD11b Recombinant Rabbit Monoclonal Antibody [JE01-17]
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WB
-
IHC-P
-
Human
-
Mouse
-
Rat
-
unconjugated
概述
产品名称
CD11b Recombinant Rabbit Monoclonal Antibody [JE01-17]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within human CD11b aa 17-66 / 1,152.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P
分子量
Predicted band size: 127 kDa
阳性对照
RAW264.7 cell lysate, J774A.1 cell lysate, human cevical cancer tissue, human slpeen tissue, human tonsil tissue, mouse spleen tissue, rat spleen tissue.
偶联
unconjugated
克隆号
JE01-17
反应性数据
| WB | IHC-P | |
|---|---|---|
| Human |
|
|
| Mouse |
|
|
| Rat |
|
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:2,000
-
IHC-P
-
1:2,000-1:5,000
靶点
功能
Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens 1, 2. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration 3. In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils 4. May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity). Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development (By similarity).
背景文献
1. Halfon M et al. ITGAM rs1143679 Variant in Systemic Lupus Erythematosus Is Associated with Increased Serum Calcification Propensity. Genes (Basel). 2023 May.
2. Sobieszek G et al. Polymorphism of the ITGAM gene (rs7193943) and bioelectric impedance analysis as potential predictors of cachexia in chronic heart failure. Sci Rep. 2021 Oct.
亚细胞定位
Cell membrane, Membrane raft.
别名
antigen CD11b (p170)
Antigen CD11b p170
CD11 antigen like family member B
CD11 antigen-like family member B
CD11b
CD11b/CD18
CD49d
Cell surface glycoprotein MAC-1 subunit alpha
Complement component 3 receptor 3 subunit
Complement Component Receptor 3 Alpha
展开图片
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☑ Relative expression (RE)
Western blot analysis of CD11b on different lysates with Rabbit anti-CD11b antibody (HA722856) at 1/2,000 dilution.
Lane 1: RAW264.7 cell lysate (5 µg/Lane)
Lane 2: C2C12 cell lysate (negative) (10 µg/Lane)
Lane 3: J774A.1 cell lysate (5 µg/Lane)
Lane 4: Mouse spleen tissue lysate (20 µg/Lane)
Lane 5: Mouse heart tissue lysate (negative) (20 µg/Lane)
Lane 6: Rat spleen tissue lysate (20 µg/Lane)
Lane 7: Rat heart tissue lysate (negative) (20 µg/Lane)
Predicted band size: 127 kDa
Observed band size: 170 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722856) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human cevical cancer tissue with Rabbit anti-CD11b antibody (HA722856) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722856) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human slpeen tissue with Rabbit anti-CD11b antibody (HA722856) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722856) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD11b antibody (HA722856) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722856) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-CD11b antibody (HA722856) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722856) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-CD11b antibody (HA722856) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722856) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
-
Nerves Stimulates Crosstalk between Gastric Cancer and Group 3 Innate Lymphoid Cells to Enhance Immunosuppression
期刊: Cancer Research
DOI: 10.1158/0008-5472.CAN-25-3092
IF: 16.6
应用: mIF
反应种属: Human
发表时间: 2026 Jan
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