LAMP1 Recombinant Rabbit Monoclonal Antibody [PSH07-39]
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Catalog# HA722827
LAMP1 Recombinant Rabbit Monoclonal Antibody [PSH07-39]
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WB
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IHC-P
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IF-Tissue
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IHC-Fr
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IP
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Human
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Mouse
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Rat
概述
产品名称
LAMP1 Recombinant Rabbit Monoclonal Antibody [PSH07-39]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within mouse LAMP1 aa 371-406.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P, IF-Tissue, IHC-Fr, IP
分子量
Predicted band size: 44 kDa
阳性对照
Jurkat cell lysate, HEK-293 cell lysate, A431 cell lysate, MCF7 cell lysate, MDA-MB-231 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, C6 cell lysate, PC-12 cell lysate, human kidney tissue, mouse colon tissue, mouse kidney tissue, mouse testis tissue, rat kidney tissue.
偶联
unconjugated
克隆号
PSH07-39
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:2,000-1:5,000
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IHC-P
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1:10,000
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IF-Tissue
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1:500-1:2,000
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IHC-Fr
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1:1,000
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IP
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1-2μg/sample
靶点
功能
Lysosomal-associated membrane protein 1 (LAMP-1) also known as lysosome-associated membrane glycoprotein 1 and CD107a (Cluster of Differentiation 107a), is a protein that in humans is encoded by the LAMP1 gene. The human LAMP1 gene is located on the long arm (q) of chromosome 13 at region 3, band 4 (13q34). Lysosomal-associated membrane protein 1 is a glycoprotein from a family of Lysosome-associated membrane glycoproteins. The LAMP-1 glycoprotein is a type I transmembrane protein which is expressed at high or medium levels in at least 76 different normal tissue cell types. It resides primarily across lysosomal membranes, and functions to provide selectins with carbohydrate ligands. CD107a has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells, and may also play a role in tumor cell differentiation and metastasis.
背景文献
1. Chaudhry N et al. Lamp1 mediates lipid transport, but is dispensable for autophagy in Drosophila. Autophagy. 2022 Oct
2. Sanmarco LM et al. Gut-licensed IFNgamma(+) NK cells drive LAMP1(+)TRAIL(+) anti-inflammatory astrocytes. Nature. 2021 Feb
亚细胞定位
Lysosome membrane, Endosome membrane, Late endosome membrane, Cell membrane, Cytolytic granule membrane.
别名
CD107 antigen like family member A antibody
CD107 antigen-like family member A antibody
CD107a antibody
CD107a antigen antibody
LAMP 1 antibody
LAMP-1 antibody
LAMP1 antibody
LAMP1_HUMAN antibody
LAMPA antibody
LGP120 antibody
展开图片
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☑ Cell treatment (CT)
Western blot analysis of LAMP1 on different lysates with Rabbit anti-LAMP1 antibody (HA722827) at 1/2,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: A431 cell lysate
Lane 4: MCF7 cell lysate
Lane 5: MDA-MB-231 cell lysate
Lane 6: HeLa cell lysate
Lane 7: HeLa cell lysate treated with deglycosylation
Lane 8: NIH/3T3 cell lysate
Lane 9: NIH/3T3 cell lysate treated with deglycosylation
Lysates/proteins at 20 µg/Lane.
Predicted band size: 44 kDa
Observed band size: 120/44 kDa
Exposure time: 21 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722827) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of LAMP1 on different lysates with Rabbit anti-LAMP1 antibody (HA722827) at 1/2,000 dilution.
Lane 1: NIH/3T3 cell lysate
Lane 2: L6 cell lysate
Lane 1: C6 cell lysate
Lane 2: PC-12 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 44 kDa
Observed band size: 100 kDa
Exposure time: 42 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722827) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Application: IHC-Fr
Species: Mouse
Site: Kidney
Sample: Frozen section
Antibody concentration: 1/1,000
Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. -
Application: IHC-Fr
Species: Rat
Site: Kidney
Sample: Frozen section
Antibody concentration: 1/1,000
Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-LAMP1 antibody (HA722827) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722827) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-LAMP1 antibody (HA722827) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722827) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-LAMP1 antibody (HA722827) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722827) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-LAMP1 antibody (HA722827) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722827) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-LAMP1 antibody (HA722827) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722827) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
LAMP1 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA722827 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722827 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (input)
Lane 2: HA722827 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of HA722827 in HeLa cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 10 seconds; ECL: K1801
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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