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GABA B Receptor 1 Recombinant Rabbit Monoclonal Antibody [JE60-09]

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Catalog# HA721338

GABA B Receptor 1 Recombinant Rabbit Monoclonal Antibody [JE60-09]

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

  • Mouse

概述

产品名称

GABA B Receptor 1 Recombinant Rabbit Monoclonal Antibody [JE60-09]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Recombinant protein within Human GABA B Receptor 1 aa 35-134 / 961.

种属反应性

Human, Mouse

验证应用

WB, IHC-P, IF-Tissue

分子量

Predicted band size: 108 kDa

阳性对照

K-562 cell lysate, Jurkat cell lysate, HeLa cell lysate, SHG-44 cell lysate, mouse brain tissue.

偶联

unconjugated

克隆号

JE60-09

RRID

AB_3072455

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

  • IF-Tissue

  • 1:100

靶点

功能

Gamma-aminobutyric acid B receptor, 1 (GABAB1), is a G-protein coupled receptor subunit encoded by the GABBR1 gene. GABAB1 is a receptor for Gamma-aminobutyric acid. Upon binding, GABAB1 will produce a slow and prolonged inhibitory effect. GABAB1 is one part of a heterodimer, which is the GABAB receptor, consisting of it and the related GABAB2 protein. The GABA(B) receptor 1 gene is mapped to chromosome 6p21.3 within the HLA class I region close to the HLA-F gene. Susceptibility loci for multiple sclerosis, epilepsy, and schizophrenia have also been mapped in this region. Alternative splicing of this gene generates 4 transcript variants. GABBR1 has been shown to interact with ATF4 and GABBR2.

背景文献

1. Shao L et al. The neurotransmitter receptor Gabbr1 regulates proliferation and function of hematopoietic stem and progenitor cells. Blood. 2021 Feb

2. Cediel ML et al. GABBR1 monoallelic de novo variants linked to neurodevelopmental delay and epilepsy. Am J Hum Genet. 2022 Oct

亚细胞定位

Cell membrane, Postsynaptic cell membrane, Cell projection, dendrite; Secreted.

UNIPROT #

SwissProt: Q9UBS5 Human

SwissProt: Q9WV18 Mouse

别名

dJ271M21.1.1 antibody

dJ271M21.1.2 antibody

FLJ92613 antibody

GABA-B receptor 1 antibody

GABA-B-R1 antibody

GABA-BR1 antibody

GABAB R1 antibody

GABAB subunit 1c antibody

GABABR1 antibody

GABBR1 3 antibody

展开

图片

  • Western blot analysis of GABA B Receptor 1 on different lysates with Rabbit anti-GABA B Receptor 1 antibody (<a href="/products/HA721338" style="font-weight: bold;text-decoration: underline;">HA721338</a>) at 1/1,000 dilution.<br /><br />Lane 1: K-562 cell lysate<br />Lane 2: Jurkat cell lysate<br />Lane 3: HeLa cell lysate<br />Lane 4: SHG-44 cell lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 108 kDa<br />Observed band size: 108 kDa<br /><br />Exposure time: 5 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721338" style="font-weight: bold;text-decoration: underline;">HA721338</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of GABA B Receptor 1 on different lysates with Rabbit anti-GABA B Receptor 1 antibody (HA721338) at 1/1,000 dilution.

    Lane 1: K-562 cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: HeLa cell lysate
    Lane 4: SHG-44 cell lysate

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 108 kDa
    Observed band size: 108 kDa

    Exposure time: 5 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721338) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-GABA B Receptor 1 antibody (<a href="/products/HA721338" style="font-weight: bold;text-decoration: underline;">HA721338</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721338" style="font-weight: bold;text-decoration: underline;">HA721338</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-GABA B Receptor 1 antibody (HA721338) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721338) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling GABA B Receptor 1 with Rabbit anti-GABA B Receptor 1 antibody (<a href="/products/HA721338" style="font-weight: bold;text-decoration: underline;">HA721338</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/HA721338" style="font-weight: bold;text-decoration: underline;">HA721338</a>, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling GABA B Receptor 1 with Rabbit anti-GABA B Receptor 1 antibody (HA721338) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721338, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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