GABA B Receptor 1 Recombinant Rabbit Monoclonal Antibody [PSH03-87]

☑ Relative expression (RE)

Western blot analysis of GABA B Receptor 1 on different lysates with Rabbit anti-GABA B Receptor 1 antibody (HA722053) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.

Lane 1: Human brain tissue lysate
Lane 2: Mouse brain tissue lysate (hot lysis)
Lane 3: Rat brain tissue lysate (no heat)
Lane 4: Mouse cerebellum tissue lysate (70℃ heat)
Lane 5: Rat cerebellum tissue lysate
Lane 6: Mouse skeletal muscle tissue lysate (no heat) (negative)
Lane 7: Rat skeletal muscle tissue lysate (no heat) (negative)

Notice: no heat means the lysate is not boiled.

Lysates/proteins at 40 µg/Lane.

Predicted band size: 108 kDa
Observed band size: 95-108 kDa

Exposure time: Lane 1-7 (left): 28 seconds; Lane 1-5 (right): 2 minutes 34 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722053) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunofluorescence analysis of paraffin-embedded mouse cerebellum tissue labeling GABA B Receptor 1 with Rabbit anti-GABA B Receptor 1 antibody (HA722053) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722053, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

☑ Relative expression (RE)

Western blot analysis of GABA B Receptor 1 on different lysates with Rabbit anti-GABA B Receptor 1 antibody (HA722053) at 1/5,000 dilution.

Lane 1: Human brain tissue lysate (40 µg/Lane)
Lane 2: Mouse brain tissue lysate (no heat) (20 µg/Lane)
Lane 3: Rat brain tissue lysate (no heat) (20 µg/Lane)
Lane 4: Mouse cerebellum tissue lysate (70℃ heat) (20 µg/Lane)
Lane 5: Rat cerebellum tissue lysate (20 µg/Lane)
Lane 6: Mouse skeletal muscle tissue lysate (no heat) (negative) (20 µg/Lane)
Lane 7: Rat skeletal muscle tissue lysate (no heat) (negative) (20 µg/Lane)

Notice: no heat means the lysate is not boiled.

Predicted band size: 108 kDa
Observed band size: 95-108 kDa

Exposure time: 4 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722053) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-GABA B Receptor 1 antibody (HA722053) at 1/20,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722053) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-GABA B Receptor 1 antibody (HA722053) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722053) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-GABA B Receptor 1 antibody (HA722053) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722053) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.