Histone H3 Recombinant Antibody [6-A7-R] - Chicken IgY (Chimeric)

Rmb: 699 1500 特惠 1500

产品规格

50μl

100μl

点击询价

联系我们

邮箱: market@huabio.cn

技术支持电话：0571-86052305

0571-89986345

市场客服电话：0571-88062880

查找各地代理商

Catalog# HA601684

Histone H3 Recombinant Antibody [6-A7-R] - Chicken IgY (Chimeric)

Application

WB
IHC-P
IF-Tissue

Reactivity

Human
Mouse
Rat

BSA and Azide free

HA610463
不含抗保成分

Conjugation

unconjugated

概述

产品名称

Histone H3 Recombinant Antibody [6-A7-R] - Chicken IgY (Chimeric)

抗体类型

Recombinant Chimeric Antibody

免疫原

Synthetic peptide within Human Histone H3 aa 1-50 / 136.

种属反应性

Human, Mouse, Rat

验证应用

WB, IHC-P, IF-Tissue

靶点分子量

Predicted band size: 15 kDa

阳性对照

HeLa (Human cervical adenocarcinoma cell) cell lysate, A549 (Human lung adenocarcinoma cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate, PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate, C6 (Rat glioma cell) cell lysate.

偶联

unconjugated

克隆号

6-A7-R

反应性数据

Tested

已验证（内部验证通过）

Published

文献已报道（未内部验证，但有文献支持）

Predicted

预测可反应（基于高同源性）

Not recommended

不推荐（内部验证未通过）

	WB	IHC-P	IF-Tissue
human
mouse
rat

产品特性

形态

Liquid

浓度

批次浓度查询

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

存储缓冲液

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

纯化方式

Protein A affinity purified.

应用稀释度

WB
1:5,000

IHC-P
1:200

IF-Tissue
1:500

靶点

功能

The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

背景文献

1. Flanagan J.F., Mi L.-Z., Chruszcz M., Cymborowski M., Clines K.L., Kim Y., Minor W., Rastinejad F., Khorasanizadeh S."Double chromodomains cooperate to recognize the methylated histone H3 tail."Nature 438:1181-1185(2005)

2. "Arginine methylation of the histone H3 tail impedes effector binding."Iberg A.N., Espejo A., Cheng D., Kim D., Michaud-Levesque J., Richard S., Bedford M.T.J. Biol. Chem. 283:3006-3010(2008)

亚细胞定位

Nucleus.

UNIPROT

SwissProt: P68431 human

SwissProt: P84243 human

SwissProt: Q16695 human

SwissProt: Q6NXT2 human

SwissProt: Q71DI3 human

SwissProt: P68433 mouse

SwissProt: P84228 mouse

SwissProt: Q6LED0 rat

别名

H3 histone family member E pseudogene antibody

H3 histone family, member A antibody

H3/A antibody

H31_HUMAN antibody

H3F3 antibody

H3FA antibody

Hist1h3a antibody

HIST1H3B antibody

HIST1H3C antibody

HIST1H3D antibody

展开

图片

Western blot analysis of Histone H3 on different lysates with Chicken anti-Histone H3 antibody (HA601684) at 1/5,000 dilution.

Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 2: A549 (Human lung adenocarcinoma cell) cell lysate
Lane 3: NIH/3T3 (Mouse fibroblast) cell lysate
Lane 4: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate
Lane 5: C6 (Rat glioma cell) cell lysate

Lysates/proteins at 15 µg/Lane.
Exposure time: 3 minutes; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA601684, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Chicken IgY IgG-HRP (HA1026), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 15 kDa
Observed band size: 15 kDa
Application: Immunohistochemistry (IHC-P)

Species: Rat
Tissue: Brain
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601684, 1/200, 1 hour at room temperature.
Secondary antibody: HA1146, 20 minutes at room temperature.
Application: Immunofluorescence (IF-tissue)

Species: Mouse
Tissue: Kidney
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA601684, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Chicken IgY (iFluor™ 488, HA1139), 1.5 hours at room temperature.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Histone H3 Recombinant Antibody [6-A7-R] - Chicken IgY (Chimeric)

Rmb: 699 1500 特惠 1500

产品规格

联系我们

概述

产品名称

抗体类型

免疫原

种属反应性

验证应用

靶点分子量

阳性对照

偶联

克隆号

反应性数据

产品特性

形态

浓度

存放说明

存储缓冲液

纯化方式

应用稀释度

靶点

功能

背景文献

亚细胞定位

UNIPROT

别名

图片

同靶点 & 同通路的产品

Histone H3 (mono+di+tri methyl K36) Recombinant Rabbit Monoclonal Antibody [PSH07-14] - ChIP Grade

Application: WB,IHC-P,IF-Cell,FC,ChIP

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

Histone H3 Mouse Monoclonal Antibody [A11-D7]

Application: WB,IF-Cell,IHC-P,IF-Tissue

Reactivity: Human,Mouse,Rat,Zebrafish

Conjugate: unconjugated

Histone H3 (tri methyl K27) Recombinant Rabbit Monoclonal Antibody [PSH28-13] - BSA and Azide free

Application: WB,IF-Cell

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

Histone H3 Mouse Monoclonal Antibody [6-A7]

Application: WB,IHC-P,IF-Tissue,ChIP

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

Histone H3 (acetyl K23) Recombinant Rabbit Monoclonal Antibody [JE46-39]

Application: WB,IF-Cell

Reactivity: Human,Mouse

Conjugate: unconjugated

Histone H3 (tri methyl K36) Recombinant Rabbit Monoclonal Antibody [PSH15-01] - ChIP Grade

Application: WB,IHC-P,IF-Cell,IP,Dot Blot,ChIP

Reactivity: Human,Mouse,Rat,Green monkey

Conjugate: unconjugated

Histone H3 (di methyl K9) Recombinant Rabbit Monoclonal Antibody [SN07-30] - BSA and Azide free

Application: WB,IF-Cell,IF-Tissue,IHC-P

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

Histone H3 (acetyl K14) Recombinant Rabbit Monoclonal Antibody [JU43-26] - ChIP Grade

Application: WB,IF-Cell,IF-Tissue,IHC-P,IP,SNAP-ChIP,CUT&Tag-seq

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

Histone H3 (tri methyl K4) Recombinant Rabbit Monoclonal Antibody [PSH25-68] - BSA and Azide free

Application: WB,IF-Cell,IHC-P,FC(Intra),ChIP

Reactivity: Human,Mouse,Rat,Green monkey

Conjugate: unconjugated

Formyl-Histone H3 (K122) Recombinant Rabbit Monoclonal Antibody [PSH27-72]

Application: WB,IHC-P

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

Histone H3 (mono methyl K18) Recombinant Rabbit Monoclonal Antibody [SA42-07]

Application: WB,IF-Cell,IF-Tissue,IHC-P

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

L-Lactyl-Histone H3 (K18) Recombinant Rabbit Monoclonal Antibody [PSH26-77] - ChIP Grade

Application: WB,IP,Dot Blot,ChIP

Reactivity: Human,Mouse

Conjugate: unconjugated

Histone H3 (acetyl K14) Recombinant Rabbit Monoclonal Antibody [JU43-26] - BSA and Azide free

Application: WB,IF-Cell,IF-Tissue,IHC-P,IP,SNAP-ChIP,CUT&Tag-seq