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NF-H Recombinant Antibody [JF99-07] - Rat IgG1 (Chimeric)

RMB: 699 特惠 1500

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Catalog# HA601582

NF-H Recombinant Antibody [JF99-07] - Rat IgG1 (Chimeric)

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

概述

产品名称

NF-H Recombinant Antibody [JF99-07] - Rat IgG1 (Chimeric)

抗体类型

Recombinant Chimeric Antibody

免疫原

Synthetic peptide within human NEFH aa 690-740.

种属反应性

Human, Mouse, Rat

验证应用

WB, IHC-P, IF-Tissue

分子量

Predicted band size: 112 kDa

阳性对照

Mouse cerebellum tissue lysate, Rat brain tissue lysate, mouse cerebellum tissue, rat cerebellum tissue.

偶联

unconjugated

克隆号

JF99-07

反应性数据

WB IHC-P IF-Tissue
Human
Tested Tested
Tested Tested
Mouse
Tested Tested
Tested Tested
Not Recommended
Rat
Tested Tested
Tested Tested
Not Recommended

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:2,000

  • IHC-P

  • 1:500-1:2,000

  • IF-Tissue

  • 1:500

靶点

功能

Neurofilament-H (NF-H), for neurofilament heavy polypeptide, a member of the intermediate filament family, is a major component of neuronal cytoskeletons. Neurofilaments are dynamic structures; they contain phosphorylation sites for a large number of protein kinases, including protein kinase A, protein kinase C, cyclin-dependent kinase 5, extracellular signal regulated kinase, glycogen synthase kinase-3, and stress-activated protein kinase gamma. In addition to their role in the control of axon caliber, neurofilaments may affect other cytoskeletal elements, such as microtubules and Actin filaments. Changes in neurofilament phosphorylation or metabolism are frequently observed in neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), Parkinson's disease and Alzheimer's disease.

背景文献

1. Wang J et al. Unveiling the NEFH+ malignant cell subtype: Insights from single-cell RNA sequencing in prostate cancer progression and tumor microenvironment interactions. Front Immunol. 2024 Dec

2. Koudonas A et al. Methylation of PCDH17 and NEFH as prognostic biomarker for nonmetastatic RCC: A cohort study. Medicine (Baltimore). 2022 Jul

亚细胞定位

Cytoplasm, cytoskeleton, Cell projection, axon.

UNIPROT

SwissProt: P12036 Human

SwissProt: P19246 Mouse

SwissProt: P16884 Rat

别名

200 kDa neurofilament protein antibody

CMT2CC antibody

Nefh antibody

Neurofilament heavy polypeptide 200kDa antibody

Neurofilament heavy polypeptide antibody

Neurofilament triplet H protein antibody

NF H antibody

NF-H antibody

NFH antibody

NFH_HUMAN antibody

图片

  • Western blot analysis of NF-H on different lysates with Rat anti-NF-H antibody (<a href="/products/HA601582" style="font-weight: bold;text-decoration: underline;">HA601582</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse cerebellum tissue lysate (10 µg/Lane)<br />Lane 2: Rat brain tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 112 kDa<br />Observed band size: 200 kDa<br /><br />Exposure time: 5 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601582" style="font-weight: bold;text-decoration: underline;">HA601582</a>) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (<a href="/products/HA1023" style="font-weight: bold;text-decoration: underline;">HA1023</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of NF-H on different lysates with Rat anti-NF-H antibody (HA601582) at 1/2,000 dilution.

    Lane 1: Mouse cerebellum tissue lysate (10 µg/Lane)
    Lane 2: Rat brain tissue lysate (20 µg/Lane)

    Predicted band size: 112 kDa
    Observed band size: 200 kDa

    Exposure time: 5 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601582) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rat anti-NF-H antibody (<a href="/products/HA601582" style="font-weight: bold;text-decoration: underline;">HA601582</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601582" style="font-weight: bold;text-decoration: underline;">HA601582</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rat anti-NF-H antibody (HA601582) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601582) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rat anti-NF-H antibody (<a href="/products/HA601582" style="font-weight: bold;text-decoration: underline;">HA601582</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601582" style="font-weight: bold;text-decoration: underline;">HA601582</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rat anti-NF-H antibody (HA601582) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601582) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: IF-Tissue<br /><br />Species: Human<br /><br />Site: brain<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/500

    Application: IF-Tissue

    Species: Human

    Site: brain

    Sample: Paraffin-embedded section

    Antibody concentration: 1/500

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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