NF-H Recombinant Antibody [JF99-07] - Guinea pig IgG2 (Chimeric)
Catalog# HA601581
NF-H Recombinant Antibody [JF99-07] - Guinea pig IgG2 (Chimeric)
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
概述
产品名称
NF-H Recombinant Antibody [JF99-07] - Guinea pig IgG2 (Chimeric)
抗体类型
Recombinant Chimeric Antibody
免疫原
Synthetic peptide within human NEFH aa 690-740.
种属反应性
Human, Mouse, Rat
验证应用
IHC-P
分子量
Predicted band size: 112 kDa
阳性对照
Human cerebellum tissue, mouse cerebellum tissue, rat cerebellum tissue.
偶联
unconjugated
克隆号
JF99-07
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
纯化方式
Protein A affinity purified.
应用稀释度
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IHC-P
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1:2,000
靶点
功能
Neurofilament-H (NF-H), for neurofilament heavy polypeptide, a member of the intermediate filament family, is a major component of neuronal cytoskeletons. Neurofilaments are dynamic structures; they contain phosphorylation sites for a large number of protein kinases, including protein kinase A, protein kinase C, cyclin-dependent kinase 5, extracellular signal regulated kinase, glycogen synthase kinase-3, and stress-activated protein kinase gamma. In addition to their role in the control of axon caliber, neurofilaments may affect other cytoskeletal elements, such as microtubules and Actin filaments. Changes in neurofilament phosphorylation or metabolism are frequently observed in neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), Parkinson's disease and Alzheimer's disease.
背景文献
1. Wang J et al. Unveiling the NEFH+ malignant cell subtype: Insights from single-cell RNA sequencing in prostate cancer progression and tumor microenvironment interactions. Front Immunol. 2024 Dec
2. Koudonas A et al. Methylation of PCDH17 and NEFH as prognostic biomarker for nonmetastatic RCC: A cohort study. Medicine (Baltimore). 2022 Jul
亚细胞定位
Cytoplasm, cytoskeleton, Cell projection, axon.
别名
200 kDa neurofilament protein antibody
CMT2CC antibody
Nefh antibody
Neurofilament heavy polypeptide 200kDa antibody
Neurofilament heavy polypeptide antibody
Neurofilament triplet H protein antibody
NF H antibody
NF-H antibody
NFH antibody
NFH_HUMAN antibody
图片
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Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Guinea pig anti-NF-H antibody (HA601581) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601581) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Guinea pig anti-NF-H antibody (HA601581) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601581) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Guinea pig anti-NF-H antibody (HA601581) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601581) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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