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HRP Conjugated Goat anti-Rat IgG(H+L) polyclonal Antibody

RMB: 200

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Catalog# HA1023

HRP Conjugated Goat anti-Rat IgG(H+L) polyclonal Antibody

Application

  • WB

  • ELISA

Reactivity

  • Rat

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

HRP Conjugated Goat anti-Rat IgG(H+L) polyclonal Antibody

抗体类型

Goat Polyclonal Antibody

免疫原

Rat IgG(H+L).

种属反应性

Rat

验证应用

WB, ELISA

偶联

HRP

产品特性

形态

Liquid

浓度

2ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*TBS (pH7.4), 0.5%BSA, 40% Glycerol.

亚型

IgG

纯化方式

Immunogen affinity purified.

应用稀释度

  • WB

  • 1:5,000

  • ELISA

  • 1:5,000- 1:10,000

发表文章中的应用

WB See 1 publications below
Dot Blot See 1 publications below

发表文章中的种属

Zebrafish See 1 publications below
Mouse See 1 publications below

靶点

功能

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.

背景文献

暂无

图片

  • Western blot analysis of Goat anti-Rat IgG(H+L) on rat IgG with Goat anti-Rat IgG(H+L) antibody (<a href="/products/HA1023" style="font-weight: bold;text-decoration: underline;">HA1023</a>) at different dilutions.<br /><br />Lane 1: Rat IgG at 1:1,000 dilution<br />Lane 2: Rat IgG at 1:5,000 dilution<br /><br />Lysates/proteins at 50 ng/Lane.<br /><br />Exposure time: 1 minute;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA1023" style="font-weight: bold;text-decoration: underline;">HA1023</a>) at different dilutions were used in 5% NFDM/TBST at room temperature for 2 hours.

    Western blot analysis of Goat anti-Rat IgG(H+L) on rat IgG with Goat anti-Rat IgG(H+L) antibody (HA1023) at different dilutions.

    Lane 1: Rat IgG at 1:1,000 dilution
    Lane 2: Rat IgG at 1:5,000 dilution

    Lysates/proteins at 50 ng/Lane.

    Exposure time: 1 minute;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA1023) at different dilutions were used in 5% NFDM/TBST at room temperature for 2 hours.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. WB
  2. Dot Blot