LAMP1 Recombinant Rabbit Monoclonal Antibody [JJ0940]
Catalog# ET1701-94
LAMP1 Recombinant Rabbit Monoclonal Antibody [JJ0940]
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WB
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IHC-P
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IF-Tissue
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Human
概述
产品名称
LAMP1 Recombinant Rabbit Monoclonal Antibody [JJ0940]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human LAMP1 aa 84-206 / 417.
种属反应性
Human
验证应用
WB, IHC-P, IF-Tissue
分子量
Predicted band size: 45 kDa
阳性对照
HeLa cell lysate, Jurkat cell lysate, HepG2 cell lysate, HUVEC cell lysate, human kidney tissue lysate, human kidney tissue.
偶联
unconjugated
克隆号
JJ0940
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000-1:5,000
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IHC-P
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1:5,000
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IF-Tissue
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1:200-1:1,000
发表文章中的应用
| WB | 查看 1 篇文献如下 |
| IHC | 查看 1 篇文献如下 |
发表文章中的种属
| Pelodiscus sinensis | 查看 1 篇文献如下 |
靶点
功能
Lysosomal-associated membrane protein 1 (LAMP-1) also known as lysosome-associated membrane glycoprotein 1 and CD107a (Cluster of Differentiation 107a), is a protein that in humans is encoded by the LAMP1 gene. The human LAMP1 gene is located on the long arm (q) of chromosome 13 at region 3, band 4 (13q34).Immunofluorescence staining of HeLa Cells with antibody to reveal lysosomal LAMP1 in red and vimentin containing intermediate filaments in green. Nuclear DNA is seen in blue. Antibodies and image courtesy EnCor Biotechnology Inc.Lysosomal-associated membrane protein 1 is a glycoprotein from a family of Lysosome-associated membrane glycoproteins.The LAMP-1 glycoprotein is a type I transmembrane protein which is expressed at high or medium levels in at least 76 different normal tissue cell types.It resides primarily across lysosomal membranes,and functions to provide selectins with carbohydrate ligands. CD107a has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells. and may also play a role in tumor cell differentiation and metastasis.
背景文献
1. Vural A et al. Activator of G-Protein Signaling 3-Induced Lysosomal Biogenesis Limits Macrophage Intracellular Bacterial Infection. J Immunol 196:846-56 (2016).
2. Rebsamen M et al. SLC38A9 is a component of the lysosomal amino acid sensing machinery that controls mTORC1. Nature 519:477-81 (2015).
序列相似性
Belongs to the LAMP family.
翻译后修饰
O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. The glycosylation of N-76 is essential for Lassa virus entry into cells.
亚细胞定位
Cell membrane, Endosome membrane, Lysosome membrane, Late endosome.
UNIPROT
别名
CD107 antigen like family member A antibody
CD107 antigen-like family member A antibody
CD107a antibody
CD107a antigen antibody
LAMP 1 antibody
LAMP-1 antibody
LAMP1 antibody
LAMP1_HUMAN antibody
LAMPA antibody
LGP120 antibody
展开图片
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Western blot analysis of LAMP1 on different lysates with Rabbit anti-LAMP1 antibody (ET1701-94) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: HepG2 cell lysate (20 µg/Lane)
Lane 4: HUVEC cell lysate (20 µg/Lane)
Lane 5: Human kidney tissue lysate (40 µg/Lane)
Predicted band size: 45 kDa
Observed band size: 100-120 kDa
Exposure time: 1 minute 2 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-94) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of LAMP1 on different lysates with Rabbit anti-LAMP1 antibody (ET1701-94) at 1/1,000 dilution.
Lane 1: HCT 116-si NT cell lysate
Lane 2: HCT 116-si LAMP1 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 45 kDa
Observed band size: 100-120 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-94) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-LAMP1 antibody (ET1701-94) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-94) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling LAMP1 with Rabbit anti-LAMP1 antibody (ET1701-94) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1701-94, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Cellular Evidence and Source of Exosomes in the Biliary System of the Chinese Soft-Shelled Turtle,Pelodiscus sinensis
Author: Ping Yang
PMID: 31507456
应用: WB,IHC
反应种属: Pelodiscus sinensis
发表时间: 2019 Aug
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Citation
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