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Bcl-2 Mouse Monoclonal Antibody [F7-1-A1]

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Catalog# M1206-4

Bcl-2 Mouse Monoclonal Antibody [F7-1-A1]

Application

  • WB

  • IF-Cell

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

Bcl-2 Mouse Monoclonal Antibody [F7-1-A1]

抗体类型

Mouse Monoclonal Antibody

免疫原

Recombinant protein within human BCL-2 aa 1-200.

种属反应性

Human, Mouse, Rat

验证应用

WB, IF-Cell, IHC-P

分子量

Predicted band size: 26 kDa

阳性对照

Jurkat cell lysate, rat spleen tissue lysate, HepG2, human breast carcinoma tissue, mouse spleen tissue.

偶联

unconjugated

克隆号

F7-1-A1

RRID

AB_3073042

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG2b

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:500

  • IF-Cell

  • 1:500

  • IHC-P

  • 1:200

靶点

功能

Bcl-2 (B-cell lymphoma 2) is the founding member of the Bcl-2 family of apoptosis regulator proteins encoded by the BCL2 gene. It has been implicated in a number of cancers, including melanoma, breast, prostate, chronic lymphocytic leukemia, and lung carcinomas, as well as schizophrenia and autoimmunity. It is also thought to be involved in resistance to conventional cancer treatment.

背景文献

1. "An inhibitor of Bcl-2 family proteins induces regression of solid tumours."Oltersdorf T., Elmore S.W., Shoemaker A.R., Armstrong R.C., Augeri D.J., Belli B.A., Bruncko M., , Rosenberg S.H.Nature 435:677-681(2005)

2. "JNK1-mediated phosphorylation of Bcl-2 regulates starvation-induced autophagy."Wei Y., Pattingre S., Sinha S., Bassik M., Levine B.Mol. Cell 30:678-688(2008)

序列相似性

Belongs to the Bcl-2 family.

组织特异性

Expressed in a variety of tissues.

翻译后修饰

Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity).; Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.; Monoubiquitinated by PRKN, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.

亚细胞定位

Mitochondrion outer membrane, Nucleus membrane, Endoplasmic reticulum membrane, Cytoplasm.

UNIPROT

SwissProt: P10415 Human

SwissProt: P10417 Mouse

SwissProt: P49950 Rat

别名

Apoptosis regulator Bcl 2 antibody

Apoptosis regulator Bcl-2 antibody

Apoptosis regulator Bcl2 antibody

AW986256 antibody

B cell CLL/lymphoma 2 antibody

B cell leukemia/lymphoma 2 antibody

Bcl-2 antibody

Bcl2 antibody

BCL2_HUMAN antibody

C430015F12Rik antibody

展开

图片

  • Western blot analysis of BCL-2 on Jurkat cell lysate using anti-BCL-2 antibody at 1/100 dilution.

    Western blot analysis of BCL-2 on Jurkat cell lysate using anti-BCL-2 antibody at 1/100 dilution.

  • Western blot analysis of Bcl-2 on rat spleen tissue lysates with Mouse anti-Bcl-2 antibody (<a href="/products/M1206-4" style="font-weight: bold;text-decoration: underline;">M1206-4</a>) at 1/500 dilution.<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 26 kDa<br />Observed band size: 26 kDa<br /><br />Exposure time: 2 minutes;<br /><br />12% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/M1206-4" style="font-weight: bold;text-decoration: underline;">M1206-4</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Bcl-2 on rat spleen tissue lysates with Mouse anti-Bcl-2 antibody (M1206-4) at 1/500 dilution.

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 26 kDa
    Observed band size: 26 kDa

    Exposure time: 2 minutes;

    12% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1206-4) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Immunofluorescent staining of HepG2 cells using anti-Bcl-2 Mouse mAb (Cat. # <a href="/products/M1206-4" style="font-weight: bold;text-decoration: underline;">M1206-4</a>).

    Immunofluorescent staining of HepG2 cells using anti-Bcl-2 Mouse mAb (Cat. # M1206-4).

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-BCL-2 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-BCL-2 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-BCL-2 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-BCL-2 antibody. Counter stained with hematoxylin.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

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