概述
产品名称
Bcl-2 Mouse Monoclonal Antibody [F7-1-A1]
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant protein within human BCL-2 aa 1-200.
种属反应性
Human, Mouse, Rat
验证应用
WB, IF-Cell, IHC-P
分子量
Predicted band size: 26 kDa
阳性对照
Jurkat cell lysate, rat spleen tissue lysate, HepG2, human breast carcinoma tissue, mouse spleen tissue.
偶联
unconjugated
克隆号
F7-1-A1
RRID
产品特性
形态
Liquid
浓度
2ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG2b
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:500
-
IF-Cell
-
1:500
-
IHC-P
-
1:200
发表文章中的应用
WB | 查看 6 篇文献如下 |
IF | 查看 1 篇文献如下 |
发表文章中的种属
Mouse | 查看 3 篇文献如下 |
Rat | 查看 3 篇文献如下 |
Human | 查看 1 篇文献如下 |
靶点
功能
Bcl-2 (B-cell lymphoma 2) is the founding member of the Bcl-2 family of apoptosis regulator proteins encoded by the BCL2 gene. It has been implicated in a number of cancers, including melanoma, breast, prostate, chronic lymphocytic leukemia, and lung carcinomas, as well as schizophrenia and autoimmunity. It is also thought to be involved in resistance to conventional cancer treatment.
背景文献
1. "An inhibitor of Bcl-2 family proteins induces regression of solid tumours."Oltersdorf T., Elmore S.W., Shoemaker A.R., Armstrong R.C., Augeri D.J., Belli B.A., Bruncko M., , Rosenberg S.H.Nature 435:677-681(2005)
2. "JNK1-mediated phosphorylation of Bcl-2 regulates starvation-induced autophagy."Wei Y., Pattingre S., Sinha S., Bassik M., Levine B.Mol. Cell 30:678-688(2008)
序列相似性
Belongs to the Bcl-2 family.
组织特异性
Expressed in a variety of tissues.
翻译后修饰
Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity).; Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.; Monoubiquitinated by PRKN, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.
亚细胞定位
Mitochondrion outer membrane, Nucleus membrane, Endoplasmic reticulum membrane, Cytoplasm.
别名
Apoptosis regulator Bcl 2 antibody
Apoptosis regulator Bcl-2 antibody
Apoptosis regulator Bcl2 antibody
AW986256 antibody
B cell CLL/lymphoma 2 antibody
B cell leukemia/lymphoma 2 antibody
Bcl-2 antibody
Bcl2 antibody
BCL2_HUMAN antibody
C430015F12Rik antibody
展开Apoptosis regulator Bcl 2 antibody
Apoptosis regulator Bcl-2 antibody
Apoptosis regulator Bcl2 antibody
AW986256 antibody
B cell CLL/lymphoma 2 antibody
B cell leukemia/lymphoma 2 antibody
Bcl-2 antibody
Bcl2 antibody
BCL2_HUMAN antibody
C430015F12Rik antibody
D630044D05Rik antibody
D830018M01Rik antibody
Leukemia/lymphoma, B-cell, 2 antibody
Oncogene B-cell leukemia 2 antibody
PPP1R50 antibody
Protein phosphatase 1, regulatory subunit 50 antibody
Bcl 2 antibody
折叠图片
-
Western blot analysis of BCL-2 on Jurkat cell lysate using anti-BCL-2 antibody at 1/100 dilution.
-
Western blot analysis of Bcl-2 on rat spleen tissue lysates with Mouse anti-Bcl-2 antibody (M1206-4) at 1/500 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1206-4) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunofluorescent staining of HepG2 cells using anti-Bcl-2 Mouse mAb (Cat. # M1206-4).
-
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-BCL-2 antibody. Counter stained with hematoxylin.
-
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-BCL-2 antibody. Counter stained with hematoxylin.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
-
Genipin promotes the apoptosis and autophagy of neuroblastoma cells by suppressing the PI3K/AKT/mTOR pathway
Author: Liu Xinying,et al
PMID: 39215133
应用: WB
反应种属: Human
发表时间: 2024 Sep
-
Citation
-
Potential role of Bcl2 in lipid metabolism and synaptic dysfunction of age-related hearing loss
Author: Yue Liu , Huasong Zhang , Cong Fan , Feiyi Liu , Shaoying Li , Juanjuan Li , Huiying Zhao , Xianhai Zeng
PMID: 37813166
应用: IF
反应种属: Rat
发表时间: 2023 Oct
-
Citation
-
MicroRNA-155 suppressed cholesterol-induced matrix degradation, pyroptosis and apoptosis by targeting RORα in nucleus pulposus cells
Author:
PMID: 37062437
应用: WB
反应种属: Rat
发表时间: 2023 Jul
-
Citation
-
Toxoplasma gondii induces MLTC-1 apoptosis via ERS pathway
Author:
PMID: 36403802
应用: WB
反应种属: Mouse
发表时间: 2023 Jan
-
Citation
-
2,2’-((1R,3R,4S)-4-methyl-4-vinylcyclohexane-1,3-diyl) bis(prop-2-en-1-amine), a bisamino derivative of β-Elemene, inhibits glioblastoma growth through downregulation of YAP signaling
Author: Cao, L. Y., Xu, J. Y., Zhuo, X. T., Zhang, W., Wei, L. J., Dong, J. H., Bai, R. R., Wang, X., Jiang, Y. Y., Wang, Y. J., Ye, X. Y., Xie, T., & Huang, Z. H.
PMID: 36628286
应用: WB
反应种属: Rat
发表时间: 2022 Dec
-
Citation
-
Luman/CREB3 knock-down inhibit hCG induced MLTC-1 apoptosis
Author: Wang, L., Meng, Q., Yang, L., Yang, D., Guo, W., Lin, P., Chen, H., Tang, K., Wang, A., & Jin, Y.
PMID: 33310232
应用: WB
反应种属: Mouse
发表时间: 2021 Feb
-
Citation
-
Deficiency of CD147 Attenuated Non-alcoholic Steatohepatitis Progression in an NLRP3-Dependent Manner
Author: Zhi-Nan Chen;Huijie Bian
PMID: 32903542
应用: WB
反应种属: Mouse
发表时间: 2020 Aug
-
Citation
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