Bcl-2 Rabbit Polyclonal Antibody

RMB: 1500 特惠 1500

产品规格

50μl

100μl

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Catalog# ER1802-97

Bcl-2 Rabbit Polyclonal Antibody

Application

WB
IF-Cell
FC

Reactivity

Human
Mouse

Conjugation

unconjugated

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

Bcl-2 Rabbit Polyclonal Antibody

抗体类型

Rabbit Polyclonal Antibody

免疫原

Synthetic peptide within human BCL-2 20-100 aa.

种属反应性

Human, Mouse

验证应用

WB, IF-Cell, FC

分子量

22/26 kDa

阳性对照

HL-60, mouse colon tissue lysate, LOVO, MCF-7, SH-SY-5Y.

偶联

unconjugated

RRID

AB_3069197

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Immunogen affinity purified.

应用稀释度

WB
1:200-1:500

IF-Cell
1:50-1:200

FC
1:50-1:100

靶点

功能

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release.

背景文献

1. Bruey J M et al. Bcl-2 and Bcl-XL regulate proinflammatory caspase-1 activation by interaction with NALP1. Cell 129:45-56 (2007).

2. Wei Y et al. JNK1-mediated phosphorylation of Bcl-2 regulates starvation-induced autophagy. Mol Cell 30:678-688 (2008).

序列相似性

Belongs to the Bcl-2 family.

组织特异性

Expressed in a variety of tissues.

翻译后修饰

Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity).; Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.; Monoubiquitinated by PRKN, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.

亚细胞定位

Mitochondrion outer membrane, Nucleus membrane, Endoplasmic reticulum membrane, Cytoplasm.

UNIPROT

SwissProt: P10415 Human

SwissProt: P10417 Mouse

别名

Apoptosis regulator Bcl 2 antibody

Apoptosis regulator Bcl-2 antibody

Apoptosis regulator Bcl2 antibody

AW986256 antibody

B cell CLL/lymphoma 2 antibody

B cell leukemia/lymphoma 2 antibody

Bcl-2 antibody

Bcl2 antibody

BCL2_HUMAN antibody

C430015F12Rik antibody

展开

图片

Western blot analysis of Bcl2 on different lysates using anti-Bcl2 antibody at 1/200 dilution.
Positive control:
Lane 1: HL-60
Lane 2: Mouse colon
ICC staining Bcl2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Bcl2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Bcl2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of MCF-7 cells with Bcl2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

按应用选择

按种属选择

Crotalaria assamica Benth alleviated acetaminophen-induced liver injury through the upregulation of Nrf2 pathway
- 期刊: JOURNAL OF ETHNOPHARMACOLOGY
- DOI: 10.1016/j.jep.2025.120664
- IF: 5.4
- 应用: WB
- 反应种属: Mouse
- 发表时间: 2025 Sept
Exosomes derived from human umbilical cord mesenchymal stem cells attenuate hepatic ischaemia-reperfusion injury via the let-7i-5p/Faslg axis
- 期刊: WORLD JOURNAL OF GASTROENTEROLOGY
- DOI: 10.3748/wjg.v31.i33.108653
- IF: 5.4
- 应用: WB
- 反应种属: Mouse,Human
- 发表时间: 2025 Sept
Apelin-13 treats postmenopausal osteoporosis by reducing mitochondrial oxidative phosphorylation and modulating the OPG/RANK/RANKL/IGF-1 pathway
- 期刊: Biochemical And Biophysical Research Communications
- DOI: 10.1016/j.bbrc.2025.152738
- IF: 2.2
- 应用: WB
- 反应种属: Mouse
- 发表时间: 2025 Sept
Dual metabolic targeting liposomes potentiate triple-negative breast cancer radiosensitivity via glucose and glutathione starvation
- 期刊: Nanomedicine: Nanotechnology, Biology and Medicine
- DOI: 10.1016/j.nano.2025.102870
- IF: 4.6
- 应用: WB
- 反应种属: Mouse
- 发表时间: 2025 Oct
1,2,3-Triazole-modified sorafenib derivatives induce DNA damage, apoptosis, and PI3K/AKT pathway suppression in hepatocellular carcinoma: Design, synthesis, and evaluation
- 期刊: Bioorganic Chemistry
- DOI: 10.1016/j.bioorg.2025.109210
- IF: 4.7
- 应用: WB
- 反应种属: Human
- 发表时间: 2025 Nov
Integrated multi-omics elucidates PRNP knockdown-mediated chemosensitization to gemcitabine in pancreatic ductal adenocarcinoma
- 期刊: Frontiers In Immunology
- DOI: 10.3389/fimmu.2025.1667835
- IF: 5.9
- 应用: WB
- 反应种属: Human
- 发表时间: 2025 Nov
Linderane from Lindera aggregata Attenuates Ulcerative Colitis by Suppressing IL-6/STAT3-Mediated Th17 Differentiation and Apoptosis Resistance
- 期刊: Journal Of Ethnopharmacology
- DOI: 10.1016/j.jep.2025.120965
- IF: 5.4
- 应用: WB
- 反应种属: Mouse
- 发表时间: 2025 Nov
Highly Soluble Mussel Foot Protein Enhances Antioxidant Defense and Cytoprotection via PI3K/Akt and Nrf2/HO-1 Pathways
- 期刊: Antioxidants
- DOI: 10.3390/antiox14060644
- IF: 6
- 应用: WB
- 反应种属: Mouse
- 发表时间: 2025 May
Rebastinib attenuates acute lung injury by promoting NLRP3 ubiquitination and blocking NLRP3/GSDMD signaling pathway in macrophages and protecting alveolar epithelial cells
- 期刊: International Immunopharmacology
- DOI: 10.1016/j.intimp.2025.114819
- IF: 4.8
- 应用: WB
- 反应种属: Mouse
- 发表时间: 2025 May
Targeting miR-144-5p/ACSM1 Axis Alleviates Doxorubicin-Induced Heart Failure by Inhibiting Lipid Peroxidation
- 期刊: Current Medical Science
- DOI: 10.1007/s11596-025-00053-z
- IF: 2
- 应用: WB
- 反应种属: Mouse,Human
- 发表时间: 2025 May
Palmatine ameliorates intestinal epithelial barrier injury in ulcerative colitis via targeting enolase 3
- 期刊: International Immunopharmacology
- DOI: 10.1016/j.intimp.2025.115110
- IF: 4.7
- 应用: WB
- 反应种属: Human
- 发表时间: 2025 Jun
Gata3 drives miR-21-5p transcription to mitigate hyperoxia-induced lung injury, independent of CpG Island methylation
- 期刊: Scientific Reports
- DOI: 10.1038/s41598-025-09039-2
- IF: 3.9
- 应用: WB
- 反应种属: Mouse
- 发表时间: 2025 Jul
Promoting ubiquitin-dependent Drp1 degradation contributes to the protective effect of Astragalin against diabetic renal fibrosis
- 期刊: Biochemical Pharmacology
- DOI: 10.1016/j.bcp.2025.117158
- IF: 5.6
- 应用: WB
- 反应种属: Mouse,Human
- 发表时间: 2025 Jul
Ursolic and oleanolic acids suppress MNNG induced malignant transformation of human gastric mucosal epithelium by regulating the PI3 K/AKT pathway
- 期刊: Scientific Reports
- DOI: 10.1038/s41598-025-03606-3
- IF: 3.9
- 应用: WB
- 反应种属: Human
- 发表时间: 2025 Jul
Pyrroloquinoline Quinone Alleviates Mitochondria Damage in Radiation-Induced Lung Injury in a MOTS-c-Dependent Manner
- 期刊: Journal Of Agricultural And Food Chemistry
- DOI:
- IF: 5.7
- 应用: WB
- 反应种属: Mouse
- 发表时间: 2024 Sep
The Mitochondrial-Derived Peptide MOTS-c Alleviates Radiation Pneumonitis via an Nrf2-Dependent Mechanism
- 期刊: Antioxidants
- DOI:
- IF: 7.7
- 应用: WB
- 反应种属: Mouse
- 发表时间: 2024 May
DNA hypomethylation of Syk induces oxidative stressand apoptosis via the PKCβ/P66shc signaling pathway indiabetic kidney disease
- 期刊: FASEB Journal
- DOI:
- IF: 4.8
- 应用: WB
- 反应种属: Human
- 发表时间: 2024 Mar
Human Amniotic Epithelial Stem Cells Alleviate Autoimmune Premature Ovarian Insufficiency in Mice by Targeting Granulosa Cells via AKT/ERK Pathways
- 期刊: Stem Cell Reviews And Reports
- DOI:
- IF: 4.8
- 应用: WB
- 反应种属: Mouse
- 发表时间: 2024 Jun
BNC1 deficiency-triggered ferroptosis through the NF2-YAP pathway induces primary ovarian insufficiency
- 期刊: Nature Communications
- DOI:
- IF: 17.694
- 应用: WB
- 反应种属: Mouse
- 发表时间: 2022 Oct
Maternal consumption of a fermented diet protects offspring against intestinal inflammation by regulating the gut microbiota
- 期刊: Gut Microbes
- DOI:
- IF: 10.245
- 应用: WB
- 反应种属: Pig
- 发表时间: 2022 May
Prescription of Sageretia hamosa Brongn Relieved Goiter through Promoted Apoptosis of Thyroid Cells via miR-511-3p and PTEN/PI3K/Akt Pathway
- 期刊: Journal Of Healthcare Engineering
- DOI:
- IF: 2.684
- 应用: WB
- 反应种属: Rat
- 发表时间: 2021 Sep
<html><html><html>Functional Analysis of the Promoter Regions of Two Apoptosis-Related Genes (<i>Bcl-2</i> and <i>Cycs</i>) and Their Regulation by Zn in Yellow Catfish. International Journal of Molecular Sciences, 22(12), 6291.</html></html></html>
- 期刊: International Journal Of Molecular Sciences
- DOI:
- IF:
- 应用: WB
- 反应种属: Yellow catfish
- 发表时间: 2021 Jun

Bcl-2 Rabbit Polyclonal Antibody

RMB: 1500 特惠 1500

产品规格

联系我们

概述

产品名称

抗体类型

免疫原

种属反应性

验证应用

分子量

阳性对照

偶联

RRID

产品特性

形态

存放说明

存储缓冲液

亚型

纯化方式

应用稀释度

靶点

功能

背景文献

序列相似性

组织特异性

翻译后修饰

亚细胞定位

UNIPROT

别名

图片

引文

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