概述
产品名称
Bcl-2 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Synthetic peptide within human BCL-2 20-100 aa.
种属反应性
Human, Mouse
验证应用
WB, IF-Cell, FC
分子量
22/26 kDa
阳性对照
HL-60, mouse colon tissue lysate, LOVO, MCF-7, SH-SY-5Y.
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
-
WB
-
1:200-1:500
-
IF-Cell
-
1:50-1:200
-
FC
-
1:50-1:100
发表文章中的应用
WB | 查看 8 篇文献如下 |
发表文章中的种属
Mouse | 查看 4 篇文献如下 |
Rat | 查看 1 篇文献如下 |
Yellow catfish | 查看 1 篇文献如下 |
靶点
功能
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release.
背景文献
1. Bruey J M et al. Bcl-2 and Bcl-XL regulate proinflammatory caspase-1 activation by interaction with NALP1. Cell 129:45-56 (2007).
2. Wei Y et al. JNK1-mediated phosphorylation of Bcl-2 regulates starvation-induced autophagy. Mol Cell 30:678-688 (2008).
序列相似性
Belongs to the Bcl-2 family.
组织特异性
Expressed in a variety of tissues.
翻译后修饰
Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity).; Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.; Monoubiquitinated by PRKN, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.
亚细胞定位
Mitochondrion outer membrane, Nucleus membrane, Endoplasmic reticulum membrane, Cytoplasm.
别名
Apoptosis regulator Bcl 2 antibody
Apoptosis regulator Bcl-2 antibody
Apoptosis regulator Bcl2 antibody
AW986256 antibody
B cell CLL/lymphoma 2 antibody
B cell leukemia/lymphoma 2 antibody
Bcl-2 antibody
Bcl2 antibody
BCL2_HUMAN antibody
C430015F12Rik antibody
展开Apoptosis regulator Bcl 2 antibody
Apoptosis regulator Bcl-2 antibody
Apoptosis regulator Bcl2 antibody
AW986256 antibody
B cell CLL/lymphoma 2 antibody
B cell leukemia/lymphoma 2 antibody
Bcl-2 antibody
Bcl2 antibody
BCL2_HUMAN antibody
C430015F12Rik antibody
D630044D05Rik antibody
D830018M01Rik antibody
Leukemia/lymphoma, B-cell, 2 antibody
Oncogene B-cell leukemia 2 antibody
PPP1R50 antibody
Protein phosphatase 1, regulatory subunit 50 antibody
Bcl 2 antibody
折叠图片
-
Western blot analysis of Bcl2 on different lysates using anti-Bcl2 antibody at 1/200 dilution.
Positive control:
Lane 1: HL-60
Lane 2: Mouse colon -
ICC staining Bcl2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
ICC staining Bcl2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
ICC staining Bcl2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
Flow cytometric analysis of MCF-7 cells with Bcl2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
-
Pyrroloquinoline Quinone Alleviates Mitochondria Damage in Radiation-Induced Lung Injury in a MOTS-c-Dependent Manner
Author: Yanli Zhang,et al
PMID: 39259217
应用: WB
反应种属: Mouse
发表时间: 2024 Sep
-
Citation
-
The Mitochondrial-Derived Peptide MOTS-c Alleviates Radiation Pneumonitis via an Nrf2-Dependent Mechanism
Author: Zhang Yanli,et al
PMID: NO PMID20240523
应用: WB
反应种属: Mouse
发表时间: 2024 May
-
Citation
-
DNA hypomethylation of Syk induces oxidative stressand apoptosis via the PKCβ/P66shc signaling pathway indiabetic kidney disease
Author: Rui Zhang,et al
PMID: 38522019
应用: WB
反应种属: Human
发表时间: 2024 Mar
-
Citation
-
Human Amniotic Epithelial Stem Cells Alleviate Autoimmune Premature Ovarian Insufficiency in Mice by Targeting Granulosa Cells via AKT/ERK Pathways
Author: Ye Xiaohang,et al
PMID: 38831179
应用: WB
反应种属: Mouse
发表时间: 2024 Jun
-
Citation
-
BNC1 deficiency-triggered ferroptosis through the NF2-YAP pathway induces primary ovarian insufficiency
Author: Wang, F., Liu, Y., Ni, F., Jin, J., Wu, Y., Huang, Y., Ye, X., Shen, X., Ying, Y., Chen, J., Chen, R., Zhang, Y., Sun, X., Wang, S., Xu, X., Chen, C., Guo, J., & Zhang, D.
PMID: 36198708
应用: WB
反应种属: Mouse
发表时间: 2022 Oct
-
Citation
-
Maternal consumption of a fermented diet protects offspring against intestinal inflammation by regulating the gut microbiota
Author: Wang, C., Wei, S., Liu, B., Wang, F., Lu, Z., Jin, M., & Wang, Y.
PMID: 35506256
应用: WB
反应种属: Pig
发表时间: 2022 May
-
Citation
-
Prescription of Sageretia hamosa Brongn Relieved Goiter through Promoted Apoptosis of Thyroid Cells via miR-511-3p and PTEN/PI3K/Akt Pathway
Author: Deng, Y. L., Chen, S., Wang, H. T., Wang, B., & Xiao, K.
PMID: 34630982
应用: WB
反应种属: Rat
发表时间: 2021 Sep
-
Citation
-
<html>Functional Analysis of the Promoter Regions of Two Apoptosis-Related Genes (<i>Bcl-2</i> and <i>Cycs</i>) and Their Regulation by Zn in Yellow Catfish. International Journal of Molecular Sciences, 22(12), 6291.</html>
Author: He, Y., Zhao, T., Chen, F., Song, C., Zhong, C., & Luo, Z.
PMID: 34208159
应用: WB
反应种属: Yellow catfish
发表时间: 2021 Jun
-
Citation
Alternative Products
同靶点 & 同通路的产品
Bcl-2 Mouse Monoclonal Antibody [9F1]
Application: WB,IHC-P
Reactivity: Human
Conjugate: unconjugated
Bcl-2 Rabbit Polyclonal Antibody
Application: WB,IF-Cell,IHC-P,FC
Reactivity: Human
Conjugate: unconjugated
Bcl-2 Mouse Monoclonal Antibody [9F3]
Application: WB,IHC-P,FC
Reactivity: Human
Conjugate: unconjugated
Bcl-2 Recombinant Rabbit Monoclonal Antibody [PD01-41]
Application: WB,IHC-P,IF-Cell,FC
Reactivity: Human
Conjugate: unconjugated
Bcl-2
Application:
Reactivity:
Conjugate:
Bcl-2 Recombinant Mouse Monoclonal Antibody [9F3-R]
Application: WB,IHC-P,FC
Reactivity: Human
Conjugate: unconjugated
Bcl-2 Rabbit Polyclonal Antibody
Application: WB,IF-Cell,IHC-P,FC
Reactivity: Human
Conjugate: unconjugated
Bcl-2 Recombinant Rabbit Monoclonal Antibody [JF104-8]
Application: WB,IF-Cell,IF-Tissue,IHC-P,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Bcl-2 Recombinant Rabbit Monoclonal Antibody [SZ10-03]
Application: WB,IF-Cell,IF-Tissue,IHC-P,IP,FC
Reactivity: Human
Conjugate: unconjugated
Bcl-2 Mouse Monoclonal Antibody [F7-1-A1]
Application: WB,IF-Cell,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
FITC Conjugated Bcl-2 Recombinant Rabbit Monoclonal Antibody [JF104-8]
Application: FC
Reactivity: Human,Mouse
Conjugate: FITC
