概述
产品名称
Bcl-2 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Synthetic peptide within human BCL2 aa 20-74.
种属反应性
Human
验证应用
WB, IF-Cell, IHC-P, FC
分子量
Predicted band size: 26 kDa
阳性对照
Hela, THP-1, HepG2, MCF-7, SH-SY5Y.
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
-
WB
-
1:500-1:1,000
-
IF-Cell
-
1:100-1:200
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
发表文章中的应用
WB | 查看 3 篇文献如下 |
发表文章中的种属
Human | 查看 2 篇文献如下 |
Mouse | 查看 1 篇文献如下 |
靶点
功能
Bcl-2 is one among many key regulators of apoptosis, which are essential for proper development, tissue homeostasis, and protection against foreign pathogens. Human Bcl-2 is an anti-apoptotic, membrane-associated oncoprotein that can promote cell survival through protein-protein interactions with other Bcl-2 related family members, such as the death suppressors Bcl-xL, Mcl-1, Bcl-w, and A1 or the death agonists Bax, Bak, Bik, Bad, and Bid. The anti-apoptotic function of Bcl-2 can also be regulated through proteolytic processing and phospho-rylation. Bcl-2 may promote cell survival by interfering with the activation of the cytochrome c/Apaf-1 pathway through stabilization of the mitochondrial membrane. Mutations in the Bcl-2 gene can contribute to cancers where normal physiological cell death mechanisms are compromised by deregulation of the anti-apoptotic influence of Bcl-2.
背景文献
1. Sun Y et al. Therapeutic effect of apocynin through antioxidant activity and suppression of apoptosis and inflammation after spinal cord injury. Exp Ther Med 13:952-960 (2017).
2. Zhang Z et al. Sepia ink oligopeptide induces apoptosis and growth inhibition in human lung cancer cells. Oncotarget 8:23202-23212 (2017).
序列相似性
Belongs to the Bcl-2 family.
组织特异性
Expressed in a variety of tissues.
翻译后修饰
Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity).; Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.; Monoubiquitinated by PRKN, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.
亚细胞定位
Mitochondrion outer membrane, Nucleus membrane, Endoplasmic reticulum membrane, Cytoplasm.
UNIPROT
别名
Apoptosis regulator Bcl 2 antibody
Apoptosis regulator Bcl-2 antibody
Apoptosis regulator Bcl2 antibody
AW986256 antibody
B cell CLL/lymphoma 2 antibody
B cell leukemia/lymphoma 2 antibody
Bcl-2 antibody
Bcl2 antibody
BCL2_HUMAN antibody
C430015F12Rik antibody
展开Apoptosis regulator Bcl 2 antibody
Apoptosis regulator Bcl-2 antibody
Apoptosis regulator Bcl2 antibody
AW986256 antibody
B cell CLL/lymphoma 2 antibody
B cell leukemia/lymphoma 2 antibody
Bcl-2 antibody
Bcl2 antibody
BCL2_HUMAN antibody
C430015F12Rik antibody
D630044D05Rik antibody
D830018M01Rik antibody
Leukemia/lymphoma, B-cell, 2 antibody
Oncogene B-cell leukemia 2 antibody
PPP1R50 antibody
Protein phosphatase 1, regulatory subunit 50 antibody
Bcl 2 antibody
折叠图片
-
Western blot analysis of BCL2 on Hela cell lysate using anti-BCL2 antibody at 1/1,000 dilution.
-
ICC staining BCL2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
ICC staining BCL2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
ICC staining BCL2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-BCL2 antibody. Counter stained with hematoxylin.
-
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-BCL2 antibody. Counter stained with hematoxylin.
-
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-BCL2 antibody. Counter stained with hematoxylin.
-
Flow cytometric analysis of MCF-7 cells with BCL2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
-
Butyrate inhibits the mitochondrial complex Ι to mediate mitochondria-dependent apoptosis of cervical cancer cells
Author:
PMID: 37370057
应用: WB
反应种属: Human
发表时间: 2023 Jun
-
Citation
-
Testicular Nuclear Receptor 4 Regulates Proliferation and Apoptosis of Bladder Cancer via Bcl-2
Author: Wang, H., Luo, W., Wang, X., Xue, D., Ren, L., Xu, L., Ge, G., Xia, L., Yu, S., Wang, M., Zhou, Z., Li, G., & Wu, H.
PMID: 34616769
应用: WB
反应种属:
发表时间: 2021 Sep
-
Citation
-
C21 steroid-enriched fraction refined from Marsdenia tenacissima inhibits hepatocellular carcinoma through the coordination of Hippo-Yap and PTEN-PI3K/AKT signaling pathways
Author: Zhen W
PMID: 29299170
应用: WB
反应种属: Human,Mouse
发表时间: 2017 Nov
-
Citation
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