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Albumin Recombinant Rabbit Monoclonal Antibody [PSH24-72]

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Catalog# HA724468

Albumin Recombinant Rabbit Monoclonal Antibody [PSH24-72]

Application

  • WB

  • IF-Cell

  • IHC-P

  • IF-Tissue

  • FC(Intra)

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

概述

产品名称

Albumin Recombinant Rabbit Monoclonal Antibody [PSH24-72]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Mouse Serum Albumin.

种属反应性

Human, Mouse, Rat

验证应用

WB, IF-Cell, IHC-P, IF-Tissue, FC(Intra)

分子量

Predicted band size: 69 kDa

阳性对照

Hep G2 (Human liver cancer cell) cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate.

偶联

unconjugated

克隆号

PSH24-72

产品特性

形态

Liquid

浓度

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:100,000

  • IF-Cell

  • 1:100

  • IHC-P

  • 1:200

  • IF-Tissue

  • 1:50

  • FC(Intra)

  • 1:200

靶点

功能

Serum albumin (ALB), the main protein in plasma, has a very good binding capacity for water, fatty acids, calcium, sodium, bilirubin, hormones, potassium and drugs. The primary function of ALB is to regulate the colloidal osmotic pressure of blood. Albumin is synthesized in the liver as preproalbumin, which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted form of albumin. Mutations in the ALB gene may result in familial dysalbuminemic hyperthyroxinemia (FDH), a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T4. FDH is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian populations.

背景文献

1. Qu N et al. Albumin Nanoparticle-Based Drug Delivery Systems. Int J Nanomedicine. 2024 Jul

2. Spada A et al. The Uniqueness of Albumin as a Carrier in Nanodrug Delivery. Mol Pharm. 2021 May

亚细胞定位

Secreted.

UNIPROT

SwissProt: P02768 Human

SwissProt: P07724 Mouse

SwissProt: P02770 Rat

别名

alb antibody

ALBU_HUMAN antibody

Albumin (32 AA) antibody

Albumin (AA 34) antibody

cell growth inhibiting protein 42 antibody

DKFZp779N1935 antibody

GIG20 antibody

GIG42 antibody

growth-inhibiting protein 20 antibody

OTTHUMP00000220436 antibody

展开

图片

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Albumin on different lysates with Rabbit anti-Albumin antibody (<a href="/products/HA724468" style="font-weight: bold;text-decoration: underline;">HA724468</a>) at 1/100,000 dilution. <br /> <br />Lane 1: Hep G2 (Human liver cancer cell) cell lysate <br />Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate <br /> <br />Lysates/proteins at 15 µg/Lane. <br />Exposure time: 10 seconds; ECL: K1801 <br /> <br />Negative expression of Albumin protein in HeLa cell is consistent with the predicted expression pattern. <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA724468" style="font-weight: bold;text-decoration: underline;">HA724468</a>, 1/100,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 69 kDa <br />Observed band size: 69 kDa

    ☑ Relative expression (RE)

    Western blot analysis of Albumin on different lysates with Rabbit anti-Albumin antibody (HA724468) at 1/100,000 dilution.

    Lane 1: Hep G2 (Human liver cancer cell) cell lysate
    Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 10 seconds; ECL: K1801

    Negative expression of Albumin protein in HeLa cell is consistent with the predicted expression pattern.

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA724468, 1/100,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 69 kDa
    Observed band size: 69 kDa

  • Western blot analysis of Albumin on different lysates with Rabbit anti-Albumin antibody (<a href="/products/HA724468" style="font-weight: bold;text-decoration: underline;">HA724468</a>) at 1/100,000 dilution. <br /> <br />Lane 1: Mouse liver tissue lysate <br />Lane 2: Rat liver tissue lysate <br /> <br />Lysates/proteins at 30 µg/Lane. <br />Exposure time: 10 seconds; ECL: K1801 <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA724468" style="font-weight: bold;text-decoration: underline;">HA724468</a>, 1/100,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 69 kDa <br />Observed band size: 69 kDa

    Western blot analysis of Albumin on different lysates with Rabbit anti-Albumin antibody (HA724468) at 1/100,000 dilution.

    Lane 1: Mouse liver tissue lysate
    Lane 2: Rat liver tissue lysate

    Lysates/proteins at 30 µg/Lane.
    Exposure time: 10 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA724468, 1/100,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 69 kDa
    Observed band size: 69 kDa

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Application: Immunocytochemistry (IF-cell) <br /> <br />Species: Human <br />Sample: Hep G2 (Human liver cancer cell)/HeLa (Human cervical adenocarcinoma cell) <br /> <br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. <br />Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. <br />Antibody dilution buffer: 1% BSA in PBST. <br /> <br />Primary antibody: <a href="/products/HA724468" style="font-weight: bold;text-decoration: underline;">HA724468</a>, 1/100, overnight at 4°C. <br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 45 minutes at room temperature. <br />Counterstain: Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). <br /> <br />Negative expression of Albumin protein in HeLa cell is consistent with the predicted expression pattern.

    ☑ Relative expression (RE)

    Application: Immunocytochemistry (IF-cell)

    Species: Human
    Sample: Hep G2 (Human liver cancer cell)/HeLa (Human cervical adenocarcinoma cell)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
    Antibody dilution buffer: 1% BSA in PBST.

    Primary antibody: HA724468, 1/100, overnight at 4°C.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
    Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).

    Negative expression of Albumin protein in HeLa cell is consistent with the predicted expression pattern.

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Rat <br />Tissue: Kidney <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724468" style="font-weight: bold;text-decoration: underline;">HA724468</a>, 1/200, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Rat
    Tissue: Kidney
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724468, 1/200, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Rat <br />Tissue: Liver <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724468" style="font-weight: bold;text-decoration: underline;">HA724468</a>, 1/200, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Rat
    Tissue: Liver
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724468, 1/200, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Application: Immunofluorescence (IF-tissue) <br /> <br />Species: Mouse <br />Tissue: Liver <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724468" style="font-weight: bold;text-decoration: underline;">HA724468</a>, 1/50, overnight at 4°C. <br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IF-tissue)

    Species: Mouse
    Tissue: Liver
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
    Primary antibody: HA724468, 1/50, overnight at 4°C.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Application: Flow Cytometry (Intra) <br /> <br />Species: Human <br />Sample: Hep G2 (Human liver cancer cells)/HeLa (Human cervix adenocarcinoma epithelial cell) <br /> <br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. <br />Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. <br />Antibody dilution buffer: 1x PBS. <br /> <br />Primary antibody: <a href="/products/HA724468" style="font-weight: bold;text-decoration: underline;">HA724468</a>(1/200, Red) compared with Rabbit IgG Isotype Control (<a href="/products/HA722127" style="font-weight: bold;text-decoration: underline;">HA722127</a>, Green), 15 minutes at room temperature. <br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 15 minutes at room temperature. <br /> <br />Negative expression of Albumin protein in HeLa cell is consistent with the predicted expression pattern.

    ☑ Relative expression (RE)

    Application: Flow Cytometry (Intra)

    Species: Human
    Sample: Hep G2 (Human liver cancer cells)/HeLa (Human cervix adenocarcinoma epithelial cell)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
    Antibody dilution buffer: 1x PBS.

    Primary antibody: HA724468(1/200, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.

    Negative expression of Albumin protein in HeLa cell is consistent with the predicted expression pattern.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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