HRP Conjugated Human/Monkey Albumin Recombinant Rabbit Monoclonal Antibody [PSH20-17] - Detector
概述
产品名称
HRP Conjugated Human/Monkey Albumin Recombinant Rabbit Monoclonal Antibody [PSH20-17] - Detector
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human Albumin.
种属反应性
Human, Dog, Cynomolgus monkey
验证应用
ELISA(Det), ELISA
阳性对照
Human Albumin protein (A3782, sigma).
偶联
HRP
克隆号
PSH20-17
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH20-16] to Human/Monkey Albumin antibody (Capture) (HA724127) and Human Albumin protein (A3782, sigma) as the standard. The reference range value is 39.06-5,000 pg/mL.
-
ELISA
-
Use at an assay dependent concentration.
靶点
功能
Serum albumin (ALB), the main protein in plasma, has a very good binding capacity for water, fatty acids, calcium, sodium, bilirubin, hormones, potassium and drugs. The primary function of ALB is to regulate the colloidal osmotic pressure of blood. Albumin is synthesized in the liver as preproalbumin, which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted form of albumin. Mutations in the ALB gene may result in familial dysalbuminemic hyperthyroxinemia (FDH), a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T4. FDH is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian populations.
背景文献
1. Zhai W et al. A1 adenosine receptor attenuates intracerebral hemorrhage-induced secondary brain injury in rats by activating the P38-MAPKAP2-Hsp27 pathway. Mol Brain 9:66 (2016).
2. Ma J et al. Pramipexole-Induced Hypothermia Reduces Early Brain Injury via PI3K/AKT/GSK3 pathway in Subarachnoid Hemorrhage rats. Sci Rep 6:23817 (2016).
亚细胞定位
Secreted.
UNIPROT
别名
alb antibody
ALBU_HUMAN antibody
Albumin (32 AA) antibody
Albumin (AA 34) antibody
cell growth inhibiting protein 42 antibody
DKFZp779N1935 antibody
GIG20 antibody
GIG42 antibody
growth-inhibiting protein 20 antibody
OTTHUMP00000220436 antibody
展开图片
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![Sandwich ELISA analysis of Human Albumin matched pair antibodies<br /><br />Capture: <a href="/products/HA724127" style="font-weight: bold;text-decoration: underline;">HA724127</a>, Human/Monkey Albumin Rabbit mAb [PSH20-16]<br />Detector: <a href="/products/HA724128" style="font-weight: bold;text-decoration: underline;">HA724128</a>, Human/Monkey Albumin Rabbit mAb [PSH20-17]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA724127" style="font-weight: bold;text-decoration: underline;">HA724127</a>) diluted in carbonate/bicarbonate buffer, at a concentHumanion of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Human Albumin protein (A3782, sigma) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA724128" style="font-weight: bold;text-decoration: underline;">HA724128</a>, HRP, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB SubstHumane for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA724128H_1.jpg?v=20260320230641)
Sandwich ELISA analysis of Human Albumin matched pair antibodies
Capture: HA724127, Human/Monkey Albumin Rabbit mAb [PSH20-16]
Detector: HA724128, Human/Monkey Albumin Rabbit mAb [PSH20-17]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724127) diluted in carbonate/bicarbonate buffer, at a concentHumanion of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Human Albumin protein (A3782, sigma) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA724128, HRP, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB SubstHumane for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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