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Osteopontin Rabbit Polyclonal Antibody

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Catalog# 0806-6

Osteopontin Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Mouse

Predicted reactivity

  • Human

  • Rat

预测的种属支持售后
This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

Osteopontin Rabbit Polyclonal Antibody

抗体类型

Rabbit Polyclonal Antibody

免疫原

Recombinant protein within human Osteopontin aa 1-314.

种属反应性

Mouse (Predicted: Human, Rat)

预测的种属支持售后

验证应用

WB

分子量

Predicted band size: 35 kDa

阳性对照

RAW264.7 treated with 100ng/mL LPS for 4 hours add 1μg/mL BFA for last 3 hours cell lysate.

偶联

unconjugated

RRID

AB_3068666

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Immunogen affinity purified.

应用稀释度

  • WB

  • 1:500-1:2,000

靶点

功能

Osteopontin (OPN), also known as bone /sialoprotein I (BSP-1 or BNSP), early T-lymphocyte activation (ETA-1), secreted phosphoprotein 1 (SPP1), 2ar and Rickettsia resistance (Ric), is a protein that in humans is encoded by the SPP1 gene (secreted phosphoprotein 1). The murine ortholog is Spp1. Osteopontin is a SIBLING (glycoprotein) that was first identified in 1986 in osteoblasts. The prefix osteo- indicates that the protein is expressed in bone, although it is also expressed in other tissues. The suffix -pontin is derived from "pons," the Latin word for bridge, and signifies osteopontin's role as a linking protein. Osteopontin is an extracellular structural protein and therefore an organic component of bone.

背景文献

1. Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;"Large-scale phosphorylation analysis of mouse liver.";Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).

2. Ashkar S., Weber G.F., Panoutsakopoulou V., Sanchirico M.E., Jansson M., Zawaideh S., Rittling S.R., Denhardt D.T., Glimcher M.J., Cantor H.;"Eta-1 (osteopontin): an early component of type-1 (cell-mediated) immunity.";Science 287:860-864(2000).

序列相似性

Belongs to the osteopontin family.

翻译后修饰

Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif.; O-glycosylated.

亚细胞定位

Secreted.

UNIPROT

SwissProt: P10451 Human

SwissProt: P10923 Mouse

SwissProt: P08721 Rat

别名

BNSP antibody

Bone sialoprotein 1 antibody

Bone sialoprotein I antibody

BSP I antibody

BSPI antibody

Early T lymphocyte activation 1 antibody

ETA 1 antibody

ETA1 antibody

MGC110940 antibody

Nephropontin antibody

展开

图片

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Osteopontin on different lysates with Rabbit anti-Osteopontin antibody (<a href="/products/0806-6" style="font-weight: bold;text-decoration: underline;">0806-6</a>) at 1/1,000 dilution.<br /><br />Lane 1: RAW264.7 cell lysate<br />Lane 2: RAW264.7 treated with 100ng/mL LPS for 4 hours, add 1μg/mL BFA for last 3 hours cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 35 kDa<br />Observed band size: 60 kDa<br /><br />Exposure time: 9 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/0806-6" style="font-weight: bold;text-decoration: underline;">0806-6</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Osteopontin on different lysates with Rabbit anti-Osteopontin antibody (0806-6) at 1/1,000 dilution.

    Lane 1: RAW264.7 cell lysate
    Lane 2: RAW264.7 treated with 100ng/mL LPS for 4 hours, add 1μg/mL BFA for last 3 hours cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 35 kDa
    Observed band size: 60 kDa

    Exposure time: 9 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0806-6) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. WB
  2. IHC-P
  3. IF

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