Osteopontin Rabbit Polyclonal Antibody

ICC staining Osteopontin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

ICC staining Osteopontin in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Osteopontin antibody. Counter stained with hematoxylin.

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Osteopontin antibody. Counter stained with hematoxylin.

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Osteopontin antibody. Counter stained with hematoxylin.

☑ Cell treatment (CT)

Immunocytochemistry analysis of RAW264.7 cells treated with LPS (100 ng/ml 6h) add BFA (300 ng/ml 3h) labeling Osteopontin with Rabbit anti-Osteopontin antibody (ER1802-16) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Osteopontin antibody (ER1802-16) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.