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Osteopontin Recombinant Rabbit Monoclonal Antibody [PSH09-23]

RMB: 699 特惠 1500

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Catalog# HA723081

Osteopontin Recombinant Rabbit Monoclonal Antibody [PSH09-23]

Application

  • IHC-Fr

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

概述

产品名称

Osteopontin Recombinant Rabbit Monoclonal Antibody [PSH09-23]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Recombinant protein within human Osteopontin aa 1-314.

种属反应性

Human

验证应用

IHC-Fr, IHC-P, IF-Tissue

分子量

Predicted band size: 35 kDa

阳性对照

Human kidney tissue, human bone tissue.

偶联

unconjugated

克隆号

PSH09-23

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • IHC-Fr

  • 1:200

  • IHC-P

  • 1:2,000

  • IF-Tissue

  • 1:200-1:500

靶点

功能

Osteopontin (OPN), also known as bone /sialoprotein I (BSP-1 or BNSP), early T-lymphocyte activation (ETA-1), secreted phosphoprotein 1 (SPP1), 2ar and Rickettsia resistance (Ric), is a protein that in humans is encoded by the SPP1 gene (secreted phosphoprotein 1). The murine ortholog is Spp1. Osteopontin is a SIBLING (glycoprotein) that was first identified in 1986 in osteoblasts. The prefix osteo- indicates that the protein is expressed in bone, although it is also expressed in other tissues. The suffix -pontin is derived from "pons," the Latin word for bridge, and signifies osteopontin's role as a linking protein. Osteopontin is an extracellular structural protein and therefore an organic component of bone.

背景文献

1. Han H et al. Macrophage-derived Osteopontin (SPP1) Protects From Nonalcoholic Steatohepatitis. Gastroenterology. 2023 Jul

2. Shirakawa K et al. Osteopontin in Cardiovascular Diseases. Biomolecules. 2021 Jul

亚细胞定位

Secreted.

UNIPROT

SwissProt: P10451 Human

SwissProt: P10923 Mouse

SwissProt: P08721 Rat

别名

BNSP antibody

Bone sialoprotein 1 antibody

Bone sialoprotein I antibody

BSP I antibody

BSPI antibody

Early T lymphocyte activation 1 antibody

ETA 1 antibody

ETA1 antibody

MGC110940 antibody

Nephropontin antibody

展开

图片

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Osteopontin antibody (<a href="/products/HA723081" style="font-weight: bold;text-decoration: underline;">HA723081</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723081" style="font-weight: bold;text-decoration: underline;">HA723081</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Osteopontin antibody (HA723081) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723081) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunofluorescence analysis of paraffin-embedded human bone tissue labeling Osteopontin with Rabbit anti-Osteopontin antibody (<a href="/products/HA723081" style="font-weight: bold;text-decoration: underline;">HA723081</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) overnight at 60℃ water bath. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723081" style="font-weight: bold;text-decoration: underline;">HA723081</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunofluorescence analysis of paraffin-embedded human bone tissue labeling Osteopontin with Rabbit anti-Osteopontin antibody (HA723081) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) overnight at 60℃ water bath. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723081) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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