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Parvalbumin Recombinant Rabbit Monoclonal Antibody [JM100-08] - BSA and Azide free

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Catalog# HA750371

Parvalbumin Recombinant Rabbit Monoclonal Antibody [JM100-08] - BSA and Azide free

Application

  • WB

  • IHC-P

  • IHC-Fr

  • IF-Tissue

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Predicted reactivity

  • Cynomolgus monkey

  • Pig

预测的种属支持售后
Conjugation

  • unconjugated

概述

产品名称

Parvalbumin Recombinant Rabbit Monoclonal Antibody [JM100-08] - BSA and Azide free

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Recombinant protein within Human Paravalbumin aa 1-110 / 110.

种属反应性

Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)

预测的种属支持售后

验证应用

WB, IHC-P, IHC-Fr, IF-Tissue, IP

靶点分子量

Predicted band size: 12 kDa

阳性对照

Mouse cerebellum tissue, rat cerebellum tissue, human cerebellum tissue, RPMI 8226 cell lysate, Mouse skeletal muscle tissue lysate, Mouse cerebellum tissue lysate, Rat cerebellum tissue lysate.

偶联

unconjugated

克隆号

JM100-08

产品特性

形态

Liquid

浓度

存放说明

Store at 2-8℃. Avoid freeze.

存储缓冲液

1*PBS (pH7.4).

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:1,000

  • IHC-P

  • 1:5,000-1:50,000

  • IHC-Fr

  • 1:500-1:1,000

  • IF-Tissue

  • 1:500

  • IP

  • 1-2μg/sample

靶点

功能

Parvalbumin (PV) is a calcium-binding protein with low molecular weight (typically 9-11 kDa). In humans, it is encoded by the PVALB gene. It is not a member of the albumin family; it is named for its size (parv-, from Latin parvus small) and its ability to coagulate. It has three EF hand motifs and is structurally related to calmodulin and troponin C. Parvalbumin is found in fast-contracting muscles, where its levels are highest, as well as in the brain and some endocrine tissues. Parvalbumin is a small, stable protein containing EF-hand type calcium binding sites. It is involved in calcium signaling. Calcium binding proteins like parvalbumin play a role in many physiological processes, namely cell-cycle regulation, second messenger production, muscle contraction, organization of microtubules and phototransduction. Therefore, calcium-binding proteins must distinguish calcium in the presence of high concentrations of other metal ions. The mechanism for the calcium selectivity has been extensively studied.

背景文献

1. Hijazi S et al. Fast-spiking parvalbumin-positive interneurons in brain physiology and Alzheimer\'s disease. Mol Psychiatry. 2023 Dec

2. Feng YF et al. Parvalbumin neurons mediate neurological phenotypes of anti-NMDAR encephalitis. Brain. 2025 May

序列相似性

Belongs to the parvalbumin family.

亚细胞定位

Axon, cytoplasm, synapse.

UNIPROT

SwissProt: P20472 Human

SwissProt: P32848 Mouse

SwissProt: P02625 Rat

别名

D22S749 antibody

MGC116759 antibody

Parvalbumin alpha antibody

PRVA_HUMAN antibody

PVALB antibody

图片

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebellum <br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500 (Parvalbumin, <a href="/products/HA750371" style="font-weight: bold;text-decoration: underline;">HA750371</a>, red); 1/500 (SLC32A1 / VGAT, <a href="/products/HA601384" style="font-weight: bold;text-decoration: underline;">HA601384</a>, green)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1/500 (Parvalbumin, HA750371, red); 1/500 (SLC32A1 / VGAT, HA601384, green)

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: Cerebellum <br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500 (Parvalbumin, <a href="/products/HA750371" style="font-weight: bold;text-decoration: underline;">HA750371</a>, red); 1/500 (SLC32A1 / VGAT, <a href="/products/HA601384" style="font-weight: bold;text-decoration: underline;">HA601384</a>, green)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Rat

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1/500 (Parvalbumin, HA750371, red); 1/500 (SLC32A1 / VGAT, HA601384, green)

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebellum <br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/1,000<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1/1,000

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: Cerebellum <br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Rat

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-Parvalbumin antibody (<a href="/products/HA750371" style="font-weight: bold;text-decoration: underline;">HA750371</a>) at 1/50,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750371" style="font-weight: bold;text-decoration: underline;">HA750371</a>) at 1/50,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-Parvalbumin antibody (HA750371) at 1/50,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750371) at 1/50,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-Parvalbumin antibody (<a href="/products/HA750371" style="font-weight: bold;text-decoration: underline;">HA750371</a>) at 1/10,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750371" style="font-weight: bold;text-decoration: underline;">HA750371</a>) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-Parvalbumin antibody (HA750371) at 1/10,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750371) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-Parvalbumin antibody (<a href="/products/HA750371" style="font-weight: bold;text-decoration: underline;">HA750371</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750371" style="font-weight: bold;text-decoration: underline;">HA750371</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-Parvalbumin antibody (HA750371) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750371) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-Parvalbumin antibody (<a href="/products/HA750371" style="font-weight: bold;text-decoration: underline;">HA750371</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750371" style="font-weight: bold;text-decoration: underline;">HA750371</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-Parvalbumin antibody (HA750371) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750371) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Parvalbumin on different lysates with Rabbit anti-Parvalbumin antibody (<a href="/products/HA750371" style="font-weight: bold;text-decoration: underline;">HA750371</a>) at 1/1,000 dilution.<br /><br />Lane 1: RPMI 8226 cell lysate (20 µg/Lane)<br />Lane 2: SK-MEL-28 cell lysate (negative) (20 µg/Lane)<br />Lane 3: Mouse skeletal muscle tissue lysate (20 µg/Lane)<br />Lane 4: Mouse cerebellum tissue lysate (40 µg/Lane)<br />Lane 5: Rat cerebellum tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 12 kDa<br />Observed band size: 14 kDa<br />Exposure time: Lane 1-3: 30 seconds; Lane 4-5: 3 minutes; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750371" style="font-weight: bold;text-decoration: underline;">HA750371</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of Parvalbumin on different lysates with Rabbit anti-Parvalbumin antibody (HA750371) at 1/1,000 dilution.

    Lane 1: RPMI 8226 cell lysate (20 µg/Lane)
    Lane 2: SK-MEL-28 cell lysate (negative) (20 µg/Lane)
    Lane 3: Mouse skeletal muscle tissue lysate (20 µg/Lane)
    Lane 4: Mouse cerebellum tissue lysate (40 µg/Lane)
    Lane 5: Rat cerebellum tissue lysate (40 µg/Lane)

    Predicted band size: 12 kDa
    Observed band size: 14 kDa
    Exposure time: Lane 1-3: 30 seconds; Lane 4-5: 3 minutes; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750371) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Application: IF-tissue<br /><br />Species: Mouse<br /><br />Site: Cerebellum <br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/500

    Application: IF-tissue

    Species: Mouse

    Site: Cerebellum

    Sample: Paraffin-embedded section

    Antibody concentration: 1/500

  • Parvalbumin was immunoprecipitated from 0.2 mg RPMI 8226 cell lysate with <a href="/products/HA750371" style="font-weight: bold;text-decoration: underline;">HA750371</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA750371" style="font-weight: bold;text-decoration: underline;">HA750371</a> at 1/1,000 dilution. Anti-Rabbit IgG for IP, AlpSdAbs&reg; VHH(HRP) (<a href="/products/025-100-005" style="font-weight: bold;text-decoration: underline;">025-100-005</a>) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: RPMI 8226 cell lysate (input)<br />Lane 2: <a href="/products/HA750371" style="font-weight: bold;text-decoration: underline;">HA750371</a> IP in RPMI 8226 cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA750371" style="font-weight: bold;text-decoration: underline;">HA750371</a> in RPMI 8226 cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 10 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    Parvalbumin was immunoprecipitated from 0.2 mg RPMI 8226 cell lysate with HA750371 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750371 at 1/1,000 dilution. Anti-Rabbit IgG for IP, AlpSdAbs® VHH(HRP) (025-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: RPMI 8226 cell lysate (input)
    Lane 2: HA750371 IP in RPMI 8226 cell lysate
    Lane 3: Rabbit IgG instead of HA750371 in RPMI 8226 cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 10 seconds; ECL: K1801

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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Conjugate: unconjugated

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