Parvalbumin Recombinant Antibody [JM100-08] - Guinea pig IgG2 (Chimeric) - BSA and Azide free
Rmb: 5600
Catalog# HA610439
Parvalbumin Recombinant Antibody [JM100-08] - Guinea pig IgG2 (Chimeric) - BSA and Azide free
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WB
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IHC-P
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Human
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Mouse
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Rat
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HA601644
含抗保成分
-
unconjugated
概述
产品名称
Parvalbumin Recombinant Antibody [JM100-08] - Guinea pig IgG2 (Chimeric) - BSA and Azide free
抗体类型
免疫原
Recombinant protein within Human Paravalbumin aa 1-110 / 110.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P
靶点分子量
Predicted band size: 12 kDa
阳性对照
RPMI8226 (Human multiple myeloma cell) cell lysate, Mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysates.
偶联
unconjugated
克隆号
JM100-08
产品特性
形态
Liquid
浓度
存放说明
Store at 2-8℃. Avoid freeze.
存储缓冲液
PBS (pH7.4).
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:5,000
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IHC-P
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1:5,000
靶点
功能
Parvalbumin (PV) is a calcium-binding protein with low molecular weight (typically 9-11 kDa). In humans, it is encoded by the PVALB gene. It is not a member of the albumin family; it is named for its size (parv-, from Latin parvus small) and its ability to coagulate. It has three EF hand motifs and is structurally related to calmodulin and troponin C. Parvalbumin is found in fast-contracting muscles, where its levels are highest, as well as in the brain and some endocrine tissues. Parvalbumin is a small, stable protein containing EF-hand type calcium binding sites. It is involved in calcium signaling. Calcium binding proteins like parvalbumin play a role in many physiological processes, namely cell-cycle regulation, second messenger production, muscle contraction, organization of microtubules and phototransduction. Therefore, calcium-binding proteins must distinguish calcium in the presence of high concentrations of other metal ions. The mechanism for the calcium selectivity has been extensively studied.
背景文献
1. Hijazi S et al. Fast-spiking parvalbumin-positive interneurons in brain physiology and Alzheimer\'s disease. Mol Psychiatry. 2023 Dec
2. Feng YF et al. Parvalbumin neurons mediate neurological phenotypes of anti-NMDAR encephalitis. Brain. 2025 May
亚细胞定位
Axon, cytoplasm, synapse.
别名
D22S749 antibody
MGC116759 antibody
Parvalbumin alpha antibody
PRVA_HUMAN antibody
PVALB antibody
图片
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☑ Relative expression (RE)
Western blot analysis of Parvalbumin on different lysates with Guinea pig anti-Parvalbumin antibody (HA610439) at 1/5,000 dilution.
Lane 1: RPMI8226 (Human multiple myeloma cell) cell lysate
Lane 2: SK-MEL-28 (Human malignant melanoma cell) cell lysate
Lane 3: Mouse skeletal muscle tissue lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 10 seconds; ECL: K1801
Negative expression of Parvalbumin protein in SK-MEL-28 cell is consistent with the predicted expression pattern.
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA610439, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Rabbit anti-Guinea pig IgG-HRP (HA1021), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 12 kDa
Observed band size: 12 kDa -
Western blot analysis of Parvalbumin on rat skeletal muscle tissue lysate with Guinea pig anti-Parvalbumin antibody (HA610439) at 1/5,000 dilution.
Lysates/proteins at 15 µg/Lane.
Exposure time: 10 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA610439, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Rabbit anti-Guinea pig IgG-HRP (HA1021), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 12 kDa
Observed band size: 12 kDa -
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Cerebellum
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA610439, 1/5,000, 1 hour at room temperature.
Secondary antibody: HA1021, 20 minutes at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Conjugate: unconjugated
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Parvalbumin Recombinant Antibody [JM100-08] - Chicken IgY (Chimeric)
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Parvalbumin Recombinant Rabbit Monoclonal Antibody [JM100-08]
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Reactivity: Human,Mouse,Rat,Cynomolgus monkey,Pig
Conjugate: unconjugated
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