SLC32A1 / VGAT Recombinant Mouse Monoclonal Antibody [PSH10-47]
Catalog# HA601384
SLC32A1 / VGAT Recombinant Mouse Monoclonal Antibody [PSH10-47]
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IHC-Fr
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IHC-P
-
Mouse
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Rat
概述
产品名称
SLC32A1 / VGAT Recombinant Mouse Monoclonal Antibody [PSH10-47]
抗体类型
Recombinant Mouse Monoclonal Antibody
种属反应性
Mouse, Rat
验证应用
IHC-Fr, IHC-P
分子量
Predicted band size: 57 kDa
阳性对照
Mouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue.
偶联
unconjugated
克隆号
PSH10-47
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
-
IHC-Fr
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1:500
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IHC-P
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1:1,000
靶点
功能
Vesicular inhibitory amino acid transporter is a protein that in humans is encoded by the SLC32A1 gene. The protein encoded by this gene is an integral membrane protein involved in gamma-aminobutyric acid (GABA) and glycine uptake into synaptic vesicles. The encoded protein is a member of amino acid/polyamine transporter family II.
背景文献
1. Heron SE et al. Association of SLC32A1 Missense Variants With Genetic Epilepsy With Febrile Seizures Plus. Neurology. 2021 May
2. Guerrini R et al. SLC32A1: One More Gene Contributing to the Solution of the Genetic Generalized Epilepsies Mystery. Neurology. 2021 May
亚细胞定位
Cytoplasmic vesicle membrane, Presynapse.
UNIPROT #
别名
bA122O1.1 antibody
GABA and glycine transporter antibody
hVIAAT antibody
SLC32A 1 antibody
Slc32a1 antibody
solute carrier family 32 (GABA vesicular transporter) member 1 antibody
Solute carrier family 32 member 1 antibody
Vesicular GABA Amino Acid Transporter antibody
Vesicular GABA transporter antibody
Vesicular inhibitory amino acid transporter antibody
展开图片
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Immunofluorescence analysis of frozen mouse cerebellum tissue with Mouse anti-SLC32A1 / VGAT antibody (HA601384) at 1/500 dilution.
The section was not undergone antigen retrieval.
The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601384, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Parvalbumin (ET1703-15, red) was stained at 1/500 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. -
Immunofluorescence analysis of frozen rat cerebellum tissue with Mouse anti-SLC32A1 / VGAT antibody (HA601384) at 1/500 dilution.
The section was not undergone antigen retrieval.
The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601384, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Parvalbumin (ET1703-15, red) was stained at 1/500 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-SLC32A1 / VGAT antibody (HA601384) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601384) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-SLC32A1 / VGAT antibody (HA601384) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601384) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-SLC32A1 / VGAT antibody (HA601384) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601384) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-SLC32A1 / VGAT antibody (HA601384) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601384) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"