Osteopontin Recombinant Rabbit Monoclonal Antibody
Catalog# IRS200RB
Osteopontin Recombinant Rabbit Monoclonal Antibody
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mIHC
-
Human
概述
产品名称
Osteopontin Recombinant Rabbit Monoclonal Antibody
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant fragment.
种属反应性
Human
验证应用
mIHC
分子量
Predicted band size: 35 kDa
阳性对照
Human tonsil tissue, human craniopharyngioma tissue.
偶联
unconjugated
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
mIHC
-
1:100
靶点
功能
Osteopontin (OPN), also known as bone /sialoprotein I (BSP-1 or BNSP), early T-lymphocyte activation (ETA-1), secreted phosphoprotein 1 (SPP1), 2ar and Rickettsia resistance (Ric), is a protein that in humans is encoded by the SPP1 gene (secreted phosphoprotein 1). The murine ortholog is Spp1. Osteopontin is a SIBLING (glycoprotein) that was first identified in 1986 in osteoblasts. The prefix osteo- indicates that the protein is expressed in bone, although it is also expressed in other tissues. The suffix -pontin is derived from "pons," the Latin word for bridge, and signifies osteopontin's role as a linking protein. Osteopontin is an extracellular structural protein and therefore an organic component of bone.
背景文献
1. Han H et al. Macrophage-derived Osteopontin (SPP1) Protects From Nonalcoholic Steatohepatitis. Gastroenterology. 2023 Jul
2. Shirakawa K et al. Osteopontin in Cardiovascular Diseases. Biomolecules. 2021 Jul
亚细胞定位
Secreted.
UNIPROT
别名
BNSP antibody
Bone sialoprotein 1 antibody
Bone sialoprotein I antibody
BSP I antibody
BSPI antibody
Early T lymphocyte activation 1 antibody
ETA 1 antibody
ETA1 antibody
MGC110940 antibody
Nephropontin antibody
展开图片
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mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Osteopontin antibody (IRS200RB) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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mIHC analysis of human craniopharyngioma tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Osteopontin antibody (IRS200RB) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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