MLH1 Recombinant Antibody [PSH20-67] - Rabbit IgG (Chimeric)
Rmb: 1500
Catalog# HA724342
MLH1 Recombinant Antibody [PSH20-67] - Rabbit IgG (Chimeric)
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IHC-P
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IF-Cell
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WB
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Human
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unconjugated
概述
产品名称
MLH1 Recombinant Antibody [PSH20-67] - Rabbit IgG (Chimeric)
抗体类型
Recombinant Chimeric Antibody
种属反应性
Human
验证应用
IHC-P, IF-Cell, WB
靶点分子量
Predicted band size: 85 kDa
阳性对照
HeLa (Human cervical adenocarcinoma cells) cell lysate, SW480 (Human colorectal zdenocarcinoma cells) cell lysate.
偶联
unconjugated
克隆号
PSH20-67
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
纯化方式
Protein A affinity purified.
应用稀释度
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IHC-P
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1:2,000
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IF-Cell
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1:3,000
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WB
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1:2,000
靶点
功能
Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.
背景文献
1. Yue Q et al. Histone H3K9 Lactylation Confers Temozolomide Resistance in Glioblastoma via LUC7L2-Mediated MLH1 Intron Retention. Adv Sci (Weinh). 2024 May
2. Guan J et al. MLH1 Deficiency-Triggered DNA Hyperexcision by Exonuclease 1 Activates the cGAS-STING Pathway. Cancer Cell. 2021 Jan
亚细胞定位
Nucleus, Chromosome.
UNIPROT
别名
COCA 2 antibody
COCA2 antibody
DNA mismatch repair protein Mlh1 antibody
FCC 2 antibody
FCC2 antibody
hMLH 1 antibody
hMLH1 antibody
HNPCC 2 antibody
HNPCC antibody
HNPCC2 antibody
展开图片
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Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Tonsil
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0) (high pressure), 2 minutes.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724342, 1/2,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Appendix
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0) (high pressure), 2 minutes.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724342, 1/2,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
☑ Relative expression (RE)
Application: Immunocytochemistry (IF-cell)
Species: Human
Sample: SW480 (Human colorectal zdenocarcinoma cells)/HCT 116 (Human colon cancer cells)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA724342, 1/3,000, overnight at 4℃.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue).
Negative control: HCT 116 (PMID: 27618981). -
☑ Relative expression (RE)
Western blot analysis of MLH1 on different lysates with Rabbit anti-MLH1 antibody (HA724342) at 1/2,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cells) cell lysate
Lane 2: HCT 116 (Human colon cancer cells) cell lysate (negative)
Lane 3: SW480 (Human colorectal zdenocarcinoma cells) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 12 seconds; ECL: K1801
HCT 116 is a negative control (PMID: 34787019).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724342, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 85 kDa
Observed band size: 85 kDa
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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