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LAMP2 Mouse Monoclonal Antibody [C9-9]

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Catalog# M1603-5

LAMP2 Mouse Monoclonal Antibody [C9-9]

Application

  • WB

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

LAMP2 Mouse Monoclonal Antibody [C9-9]

抗体类型

Mouse Monoclonal Antibody

免疫原

Recombinant protein corresponding to N terminal Human LAMP2.

种属反应性

Human

验证应用

WB, IHC-P

靶点分子量

Predicted band size: 45 kDa

阳性对照

HeLa cell lysate, Jurkat cell lysate, HepG2 cell lysate, HUVEC cell lysate, JAR cell lysate, HEK-293 cell lysate, THP-1 cell lysate, human kidney tissue, human liver tissue.

偶联

unconjugated

克隆号

C9-9

RRID

AB_3073163

产品特性

形态

Liquid

浓度

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

存储缓冲液

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG2b

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

靶点

功能

Plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live . Functions by binding target proteins, such as GAPDH and MLLT11, and targeting them for lysosomal degradation . Plays a role in lysosomal protein degradation in response to starvation (By similarity). Required for the fusion of autophagosomes with lysosomes during autophagy . Cells that lack LAMP2 express normal levels of VAMP8, but fail to accumulate STX17 on autophagosomes, which is the most likely explanation for the lack of fusion between autophagosomes and lysosomes . Required for normal degradation of the contents of autophagosomes . Required for efficient MHCII-mediated presentation of exogenous antigens via its function in lysosomal protein degradation; antigenic peptides generated by proteases in the endosomal/lysosomal compartment are captured by nascent MHCII subunits . Is not required for efficient MHCII-mediated presentation of endogenous antigens .

背景文献

1. Cuervo A.M.et.al.A receptor for the selective uptake and degradation of proteins by lysosomes.Science 273:501-503(1996).

序列相似性

Belongs to the LAMP family.

组织特异性

Isoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is detected in spleen, thymus, prostate, testis, small intestine, colon, skeletal muscle, brain, placenta, lung, kidney, ovary and pancreas and liver. Isoform LAMP-2C is detected in small intestine, colon, heart, brain, skeletal muscle, and at lower levels in kidney and placenta.

翻译后修饰

O- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans.

亚细胞定位

Cell membrane, Cytoplasmic vesicle, Endosome, Lysosome, Membrane.

UNIPROT

SwissProt: P13473 Human

别名

CD107 antigen like family member B antibody

CD107 antigen-like family member B antibody

CD107b antibody

LAMP 2 antibody

LAMP 2C antibody

LAMP-2 antibody

LAMP2 antibody

LAMP2_HUMAN antibody

LAMPB antibody

LGP 96 antibody

展开

图片

  • Western blot analysis of LAMP2 on different lysates with Mouse anti-LAMP2 antibody (<a href="/products/M1603-5" style="font-weight: bold;text-decoration: underline;">M1603-5</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: Jurkat cell lysate<br />Lane 3: HepG2 cell lysate<br />Lane 4: HUVEC cell lysate<br />Lane 5: JAR cell lysate<br />Lane 6: HEK-293 cell lysate<br />Lane 7: THP-1 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 45 kDa<br />Observed band size: 110 kDa<br /><br />Exposure time: 1 minute 2 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/M1603-5" style="font-weight: bold;text-decoration: underline;">M1603-5</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of LAMP2 on different lysates with Mouse anti-LAMP2 antibody (M1603-5) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: HepG2 cell lysate
    Lane 4: HUVEC cell lysate
    Lane 5: JAR cell lysate
    Lane 6: HEK-293 cell lysate
    Lane 7: THP-1 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 45 kDa
    Observed band size: 110 kDa

    Exposure time: 1 minute 2 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1603-5) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-LAMP2 antibody (<a href="/products/M1603-5" style="font-weight: bold;text-decoration: underline;">M1603-5</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M1603-5" style="font-weight: bold;text-decoration: underline;">M1603-5</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-LAMP2 antibody (M1603-5) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1603-5) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-LAMP2 antibody (<a href="/products/M1603-5" style="font-weight: bold;text-decoration: underline;">M1603-5</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M1603-5" style="font-weight: bold;text-decoration: underline;">M1603-5</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-LAMP2 antibody (M1603-5) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1603-5) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

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