Skip to content

LAMP2a Recombinant Rabbit Monoclonal Antibody [SA46-01]

RMB: 900 特惠 1500

产品规格

点击询价

联系我们

邮箱: market@huabio.cn

电话: 0571-88062880

查找各地代理商

Catalog# ET1601-24

LAMP2a Recombinant Rabbit Monoclonal Antibody [SA46-01]

Application

  • WB

  • IHC-P

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

LAMP2a Recombinant Rabbit Monoclonal Antibody [SA46-01]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within Human LAMP2a aa 361-410 / 410.

种属反应性

Human, Mouse, Rat

验证应用

WB, IHC-P, IP

分子量

Predicted band size: 45 kDa

阳性对照

SK-MEL-28 cell lysate, HeLa cell lysate, JAR cell lysate, U-937 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse liver tissue lysate, rat liver tissue lysate, rat lung tissue lysate, human kidney tissue, human liver tissue, human pancreas tissue, mouse kidney tissue, mouse placenta tissue, mouse pancreas tissue, rat kidney tissue.

偶联

unconjugated

克隆号

SA46-01

RRID

AB_3069607

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:5,000

  • IHC-P

  • 1:500

  • IP

  • Use at an assay dependent concentration.

靶点

功能

Lysosome-associated membrane protein 2 (LAMP2), also known as CD107b (Cluster of Differentiation 107b) and Mac-3, is a human gene. Its protein, LAMP2, is one of the lysosome-associated membrane glycoproteins. The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of the gene produces three variants - LAMP-2A, LAMP-2B and LAMP-2C. LAMP-2A is the receptor for chaperone-mediated autophagy. Recently it has been determined that antibodies against LAMP-2 account for a fraction of patients who get a serious kidney disease termed focal necrotizing glomerulonephritis. LAMP-2B is associated with Danon disease.

背景文献

1. Guan, JJ. et al. 2015. DRAM1 regulates apoptosis through increasing protein levels and lysosomal localization of BAX. Cell death & disease. 6: e1624.

2. Gu, G. et al. 2013. Ubiquitin E3 Ligase A20 is Required in Degradation of Microbial Superantigens in Vascular Endothelial Cells. Cell Biochem. Biophys. 66: 649-655.

序列相似性

Belongs to the LAMP family.

组织特异性

Isoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is detected in spleen, thymus, prostate, testis, small intestine, colon, skeletal muscle, brain, placenta, lung, kidney, ovary and pancreas and liver. Isoform LAMP-2C is detected in small intestine, colon, heart, brain, skeletal muscle, and at lower levels in kidney and placenta.

翻译后修饰

O- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans.

亚细胞定位

Cell membrane, Endosome membrane, Lysosome membrane

UNIPROT

SwissProt: P13473 Human

SwissProt: P17047 Mouse

SwissProt: P17046 Rat

别名

CD107 antigen-like family member B antibody

CD107b antibody

LAMP 2 antibody

Lamp 2a antibody

LAMP-2 antibody

LAMP2 antibody

LAMP2_HUMAN antibody

Lysosome-associated membrane glycoprotein 2 antibody

Lysosome-associated membrane protein 2 antibody

图片

  • Western blot analysis of LAMP2a on different lysates with Rabbit anti-LAMP2a antibody (<a href="/products/ET1601-24" style="font-weight: bold;text-decoration: underline;">ET1601-24</a>) at 1/5,000 dilution.<br /><br />Lane 1: SK-MEL-28 cell lysate (20 µg/Lane)<br />Lane 2: HeLa cell lysate (20 µg/Lane)<br />Lane 3: JAR cell lysate (20 µg/Lane)<br />Lane 4: U-937 cell lysate (20 µg/Lane)<br />Lane 5: RAW264.7 cell lysate (15 µg/Lane)<br />Lane 6: NIH/3T3 cell lysate (15 µg/Lane)<br />Lane 7: PC-12 cell lysate (15 µg/Lane)<br />Lane 8: Mouse liver tissue lysate (30 µg/Lane)<br />Lane 9: Rat liver tissue lysate (30 µg/Lane)<br />Lane 10: Rat lung tissue lysate (30 µg/Lane)<br /><br />Predicted band size: 45 kDa<br />Observed band size: 70-140 kDa<br /><br />Exposure time: Lane 1-4: 2 minutes 37 seconds; Lane 5-10: 5 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1601-24" style="font-weight: bold;text-decoration: underline;">ET1601-24</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of LAMP2a on different lysates with Rabbit anti-LAMP2a antibody (ET1601-24) at 1/5,000 dilution.

    Lane 1: SK-MEL-28 cell lysate (20 µg/Lane)
    Lane 2: HeLa cell lysate (20 µg/Lane)
    Lane 3: JAR cell lysate (20 µg/Lane)
    Lane 4: U-937 cell lysate (20 µg/Lane)
    Lane 5: RAW264.7 cell lysate (15 µg/Lane)
    Lane 6: NIH/3T3 cell lysate (15 µg/Lane)
    Lane 7: PC-12 cell lysate (15 µg/Lane)
    Lane 8: Mouse liver tissue lysate (30 µg/Lane)
    Lane 9: Rat liver tissue lysate (30 µg/Lane)
    Lane 10: Rat lung tissue lysate (30 µg/Lane)

    Predicted band size: 45 kDa
    Observed band size: 70-140 kDa

    Exposure time: Lane 1-4: 2 minutes 37 seconds; Lane 5-10: 5 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-24) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-LAMP2a antibody (<a href="/products/ET1601-24" style="font-weight: bold;text-decoration: underline;">ET1601-24</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1601-24" style="font-weight: bold;text-decoration: underline;">ET1601-24</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-LAMP2a antibody (ET1601-24) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-24) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-LAMP2a antibody (<a href="/products/ET1601-24" style="font-weight: bold;text-decoration: underline;">ET1601-24</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1601-24" style="font-weight: bold;text-decoration: underline;">ET1601-24</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-LAMP2a antibody (ET1601-24) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-24) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-LAMP2a antibody (<a href="/products/ET1601-24" style="font-weight: bold;text-decoration: underline;">ET1601-24</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1601-24" style="font-weight: bold;text-decoration: underline;">ET1601-24</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-LAMP2a antibody (ET1601-24) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-24) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • LAMP2a was immunoprecipitated from 0.2 mg SK-MEL-28 cell lysate with <a href="/products/ET1601-24" style="font-weight: bold;text-decoration: underline;">ET1601-24</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/ET1601-24" style="font-weight: bold;text-decoration: underline;">ET1601-24</a> at 1/2,000 dilution. HRP Conjugated Rabbit IgG kappa light chain antibody (<a href="/products/M1208-2" style="font-weight: bold;text-decoration: underline;">M1208-2</a>) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: SK-MEL-28 cell lysate (Input)<br />Lane 2: <a href="/products/ET1601-24" style="font-weight: bold;text-decoration: underline;">ET1601-24</a> IP in SK-MEL-28 cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/ET1601-24" style="font-weight: bold;text-decoration: underline;">ET1601-24</a> in SK-MEL-28 cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 20 seconds; ECL: K1801

    LAMP2a was immunoprecipitated from 0.2 mg SK-MEL-28 cell lysate with ET1601-24 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1601-24 at 1/2,000 dilution. HRP Conjugated Rabbit IgG kappa light chain antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: SK-MEL-28 cell lysate (Input)
    Lane 2: ET1601-24 IP in SK-MEL-28 cell lysate
    Lane 3: Rabbit IgG instead of ET1601-24 in SK-MEL-28 cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 20 seconds; ECL: K1801

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

同靶点 & 同通路的产品

metafield image

LAMP2a Recombinant Rabbit Monoclonal Antibody [SA46-01] - BSA and Azide free

Application: WB,IHC-P,IP

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

LAMP2a Rabbit Polyclonal Antibody

Application: WB,IHC-P

Reactivity: Human,Mouse

Conjugate: unconjugated

Copyright @ 2026 HUABIO

网站备案号：  浙ICP备09005892号-1 |  浙公网安备 33019202000643号

Close (esc)

Popup

Use this popup to embed a mailing list sign up form. Alternatively use it as a simple call to action with a link to a product or a page.

Age verification

By clicking enter you are verifying that you are old enough to consume alcohol.

Enter

搜索

主菜单