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Iba1 Recombinant Antibody [JM36-62] - Rat IgG1 (Chimeric)

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Catalog# HA601367

Iba1 Recombinant Antibody [JM36-62] - Rat IgG1 (Chimeric)

Application

  • IHC-Fr

  • IHC-P

Reactivity

  • Mouse

  • Rat

Predicted reactivity

  • Cynomolgus monkey

  • Pig

预测的种属支持售后

概述

产品名称

Iba1 Recombinant Antibody [JM36-62] - Rat IgG1 (Chimeric)

抗体类型

Recombinant Chimeric Antibody

免疫原

Synthetic peptide within N-terminal human Iba1.

产品特异性

This Rat Recombinant monoclonal chimeric antibody has been engineered from a HiMab parent antibody (ET1705-78). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed FC-reactive secondary antibodies are recommended.

种属反应性

Mouse, Rat (Predicted: Cynomolgus monkey, Pig)

预测的种属支持售后

验证应用

IHC-Fr, IHC-P

分子量

Predicted band size: 17 kDa

阳性对照

Mouse brain tissue, mouse striatum tissue, rat striatum tissue.

偶联

unconjugated

克隆号

JM36-62

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

纯化方式

Protein A affinity purified.

应用稀释度

  • IHC-Fr

  • 1:500-1:1,000

  • IHC-P

  • 1:100-1:500

靶点

功能

Ionized calcium-binding adapter molecule 1 (Iba1), also known as allograft inflammatory factor-1 (AIF-1), is a 147 amino acid cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. Iba1, containing two EF domains, is induced by cytokines and interferons. In an unstimulated state, Iba1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding Iba1 maps to chromosome 6p21.33 and resides in the tumor necrosis factor (TNF) cluster of genes located in the region represented by the human major histocompatibility complex (MHC).

背景文献

1. Hennessy E et al. Systemic TNF-a produces acute cognitive dysfunction and exaggerated sickness behavior when superimposed upon progressive neurodegeneration. Brain Behav Immun 59:233-244 (2017).

2. Arentsen T et al. The bacterial peptidoglycan-sensing molecule Pglyrp2 modulates brain development and behavior. Mol Psychiatry 22:257-266 (2017).

亚细胞定位

Cytoplasm, cytoskeleton, Cell projection, ruffle membrane, Cell projection, phagocytic cup.

UNIPROT #

SwissProt: P55008 Human

SwissProt: O70200 Mouse

SwissProt: P55009 Rat

别名

AIF 1 antibody

AIF-1 antibody

Aif1 antibody

AIF1 protein antibody

AIF1_HUMAN antibody

Allograft inflammatory factor 1 antibody

Allograft inflammatory factor 1 splice variant G antibody

allograft inflammatory factor-1 splice variant Hara-1 antibody

balloon angioplasty responsive transcription antibody

BART 1 antibody

展开

图片

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebral cortex<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1: 500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Cerebral cortex

    Sample: Frozen section

    Antibody concentration: 1: 500

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebellum<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1: 1,000 (Iba1, <a href="/products/HA601367" style="font-weight: bold;text-decoration: underline;">HA601367</a>, green); 1:1,000 (NeuN, <a href="/products/ET1602-12" style="font-weight: bold;text-decoration: underline;">ET1602-12</a>, red)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1: 1,000 (Iba1, HA601367, green); 1:1,000 (NeuN, ET1602-12, red)

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebellum<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1: 500 (Iba1, <a href="/products/HA601367" style="font-weight: bold;text-decoration: underline;">HA601367</a>, green); 1:1,000 (GLAST /EAAT1, <a href="/products/ET1704-54" style="font-weight: bold;text-decoration: underline;">ET1704-54</a>, red)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1: 500 (Iba1, HA601367, green); 1:1,000 (GLAST /EAAT1, ET1704-54, red)

    Antigen retrieval: Not required

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rat anti-Iba1 antibody (<a href="/products/HA601367" style="font-weight: bold;text-decoration: underline;">HA601367</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601367" style="font-weight: bold;text-decoration: underline;">HA601367</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rat anti-Iba1 antibody (HA601367) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601367) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse striatum tissue with Rat anti-Iba1 antibody (<a href="/products/HA601367" style="font-weight: bold;text-decoration: underline;">HA601367</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601367" style="font-weight: bold;text-decoration: underline;">HA601367</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse striatum tissue with Rat anti-Iba1 antibody (HA601367) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601367) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat striatum tissue with Rat anti-Iba1 antibody (<a href="/products/HA601367" style="font-weight: bold;text-decoration: underline;">HA601367</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601367" style="font-weight: bold;text-decoration: underline;">HA601367</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat striatum tissue with Rat anti-Iba1 antibody (HA601367) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601367) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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