Myeloperoxidase Recombinant Rabbit Monoclonal Antibody [PD00-20]
Catalog# IRS037
Myeloperoxidase Recombinant Rabbit Monoclonal Antibody [PD00-20]
-
mIHC
-
Human
概述
产品名称
Myeloperoxidase Recombinant Rabbit Monoclonal Antibody [PD00-20]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human Myeloperoxidase aa 600-700 (C terminal).
种属反应性
Human
验证应用
mIHC
分子量
Predicted band size: 84 kDa
阳性对照
Human tonsils tissue.
偶联
unconjugated
克隆号
PD00-20
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
mIHC
-
1:100
靶点
功能
Myeloperoxidase (MPO) is a peroxidase enzyme that in humans is encoded by the MPO gene on chromosome 17. MPO is most abundantly expressed in neutrophil granulocytes (a subtype of white blood cells), and produces hypohalous acids to carry out their antimicrobial activity, including hypochlorous acid, the sodium salt of which is the chemical in bleach. It is a lysosomal protein stored in azurophilic granules of the neutrophil and released into the extracellular space during degranulation. Neutrophil myeloperoxidase has a heme pigment, which causes its green color in secretions rich in neutrophils, such as mucus and sputum. The green color contributed to its outdated name verdoperoxidase. Immunohistochemical staining for myeloperoxidase used to be administered in the diagnosis of acute myeloid leukemia to demonstrate that the leukemic cells were derived from the myeloid lineage. Myeloperoxidase staining is still important in the diagnosis of myeloid sarcoma, contrasting with the negative staining of lymphomas, which can otherwise have a similar appearance.
背景文献
1. Hu CH. et. al. Small molecule and macrocyclic pyrazole derived inhibitors of myeloperoxidase (MPO). Bioorg Med Chem Lett. 2021 Jun
2. Chen S. et. al. Targeting Myeloperoxidase (MPO) Mediated Oxidative Stress and Inflammation for Reducing Brain Ischemia Injury: Potential Application of Natural Compounds. Front Physiol. 2020 May
亚细胞定位
Lysosome.
UNIPROT #
别名
84 kDa myeloperoxidase antibody
89 kDa myeloperoxidase antibody
EC 1.11.1.7 antibody
EC1.11.2.2 antibody
fj80f04 antibody
MPO antibody
mpx antibody
myeloid-specific peroxidase antibody
Myeloperoxidase antibody
Myeloperoxidase heavy chain antibody
展开图片
-
mIHC analysis of human tonsils tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Myeloperoxidase antibody (IRS037) at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
-
mIHC analysis of human lung cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with FOXP3 (IRS008), CD57 (IRS027), MPO (IRS037), CD8 (IRS007) and panCK (IRS010) antibody at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"