CD3 Recombinant Rabbit Monoclonal Antibody [JE80-02]
Catalog# IRS022
CD3 Recombinant Rabbit Monoclonal Antibody [JE80-02]
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mIHC
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Human
概述
产品名称
CD3 Recombinant Rabbit Monoclonal Antibody [JE80-02]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within human CD3E aa 158-207/207.
种属反应性
Human
验证应用
mIHC
分子量
Predicted band size: 23 kDa
阳性对照
Human tonsils tissue, human spleen tissue.
偶联
unconjugated
克隆号
JE80-02
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
mIHC
-
1:100
靶点
功能
The CD3 protein is a T-cell marker, a complex of four structurally distinct membrane glycoprotein isoforms, 20-50 kDa, comprising extracellular, transmembrane and intracellular domains. The CD3 complex is responsible for mediating signal transduction to the internal environment upon antigenic recognition by TCR, causing T-cell proliferation and release of cytokines. Except for a weak expression in Purkinje cells (with some of the Abs) and activated NK-cells, CD3 is found only in T-cells. CD3 appear in the cytoplasm of prothymocytes, and on the surface of about 95% of thymocytes, while cytoplasmic CD3 is lost as the cells differentiate into medullary thymocytes. In therapy resistant celiac disease, a shift from membranous to cytoplasmic CD3 expression is seen (together with loss of CD8). In malignant lymphomas, CD3 is a pan-T-cell lineage-restricted antigen, detected in 80-97% of the T-cell lymphomas. Mature T-cell lymphomas including cases of mycosis fungoides, peripheral T-cell lymphoma and anaplastic large cell lymphoma may aberrantly lose CD3. NK-cell lymphomas can show a cytoplasmic reaction. Reed-Sternberg cells may show a globular paranuclear reaction. CD3 is an important marker in the classification of malignant lymphomas and lymphoid leukaemias. Also the marker is useful for the identification of T-cells in, e.g., celiac disease, lymphocytic colitis and colorectal carcinomas associated with loss of a mismatch repair protein.
背景文献
1. Erman B. et. al. Biallelic Form of a Known CD3E Mutation in a Patient with Severe Combined Immunodeficiency. J Clin Immunol. 2020 Apr
2. Chen Q. et. al. CD3(+)CD20(+) T cells and their roles in human diseases. Hum Immunol. 2019 Mar
亚细胞定位
Cell membrane.
UNIPROT #
别名
4930549J05Rik antibody
A430104F18Rik antibody
AW552088 antibody
Cd247 antibody
CD247 antigen antibody
CD247 antigen, zeta subunit antibody
CD247 molecule antibody
CD3 antibody
CD3 antigen, delta subunit antibody
CD3 delta antibody
展开图片
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mIHC analysis of human tonsils tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD3 antibody (IRS022) at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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mIHC analysis of human spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD3 antibody (IRS022) at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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mIHC analysis of human lung cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with panCK (IRS010), CD3 (IRS022) and Ki67 (IRS036) antibody at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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mIHC analysis of human gastric cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD11b (IRS014), CD31 (IRS023), aSMA (IRS012), panCK (IRS010) and CD3 (IRS022) antibody at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点&同通路的产品
CD3 Recombinant Rabbit Monoclonal Antibody [JE80-02]
Application: WB,IHC-P,IF-Tissue,IP,mIHC,IF-Cell
Reactivity: Human
Conjugate: unconjugated
