LONP1 Recombinant Rabbit Monoclonal Antibody [PSH28-77] - BSA and Azide free
Rmb: 5600
Catalog# HA752123
LONP1 Recombinant Rabbit Monoclonal Antibody [PSH28-77] - BSA and Azide free
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WB
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IF-Cell
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IHC-P
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FC(Intra)
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IP
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Human
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Mouse
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Rat
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HA724808
含抗保成分
-
unconjugated
概述
产品名称
LONP1 Recombinant Rabbit Monoclonal Antibody [PSH28-77] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human LONP1 aa 560-959.
种属反应性
Human, Mouse, Rat
验证应用
WB, IF-Cell, IHC-P, FC(Intra), IP
靶点分子量
Predicted band size: 106 kDa
阳性对照
HeLa (Human cervical adenocarcinoma cell) cell lysate, HEK-293 (Human embryonic kidney cell) cell lysate, U-87 MG (Human glioblastoma cell) cell lysate, PC-3 (Human prostate cancer cell) cell lysate, Hep G2 (Human hepatocellular carcinoma cell) cell lysate, COS-1 (African green monkey kidney fibroblast) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate.
偶联
unconjugated
克隆号
PSH28-77
反应性数据
已验证（内部验证通过）
文献已报道（未内部验证，但有文献支持）
预测可反应（基于高同源性）
不推荐（内部验证未通过）
| WB | IHC-P | FC(Intra) | IF-Cell | IP | |
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| human |
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| mouse |
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| rat |
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产品特性
形态
Liquid
浓度
存放说明
Store at 2-8℃. Avoid freeze.
存储缓冲液
PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
靶点
功能
Lon protease homolog, mitochondrial is a protease, an enzyme that in humans is encoded by the LONP1 gene. Lon protease (LONP1) is a conserved serine peptidase identified from bacteria to eukaryotic cells. In mitochondrial matrix, a majority of damaged proteins is removed via proteolysis led by Lon protease, which is an essential mechanism for mitochondrial protein quality control. LONP1 is the major protease responsible for the recognition and removal of unfolded proteins in the mitochondrial matrix and thereby protects the cell from the accumulation of aggregated proteins in the mitochondrion. However LONP1 is unable to recognise or degrade model aggregated proteins. LONP1 is also a DNA-binding protein that participates in mtDNA maintenance and gene expression regulation. LONP1 degrades mitochondrial transcription factor A (TFAM) when substrate is modified by post-translational modifications (PTMs) such as phosphorylation, regulating mtDNA copy number and metabolism to maintain the TFAM/mtDNA ratio necessary to control replication and transcription.
背景文献
1. Liu Y et al. Artemisinins ameliorate polycystic ovarian syndrome by mediating LONP1-CYP11A1 interaction. Science. 2024 Jun
2. Bai M et al. LONP1 targets HMGCS2 to protect mitochondrial function and attenuate chronic kidney disease. EMBO Mol Med. 2023 Feb
亚细胞定位
Mitochondrion matrix.
别名
Lon protease homolog, mitochondrial
EC:3.4.21.53
LONHs
Lon protease-like protein UniRule annotation (LONP)
Mitochondrial ATP-dependent protease Lon
Mitochondrial chaperone LONP1
Serine protease 15
LONP1
Lon
PRSS15
图片
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This data was developed using HA724808, the same antibody clone in a different buffer formulation.
Western blot analysis of LONP1 on different lysates with Rabbit anti-LONP1 antibody (HA724808) at 1/2,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 2: HEK-293 (Human embryonic kidney cell) cell lysate
Lane 3: U-87 MG (Human glioblastoma cell) cell lysate
Lane 4: PC-3 (Human prostate cancer cell) cell lysate
Lane 5: Hep G2 (Human hepatocellular carcinoma cell) cell lysate
Lane 6: COS-1 (African green monkey kidney fibroblast) cell lysate
Lane 7: NIH/3T3 (Mouse fibroblast) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 1 minute 50 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724808, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 106 kDa
Observed band size: 106 kDa -
This data was developed using HA724808, the same antibody clone in a different buffer formulation.
Application: Immunocytochemistry (IF-cell)
Species: Human
Sample: SH-SY5Y (Human neuroblastoma cell)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA724808, 1/1,000, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: The mitochondria dyes was Mito-Tracker (Beyotime, Red). The nuclear counterstain was DAPI (Blue). -
This data was developed using HA724808, the same antibody clone in a different buffer formulation.
Application: Immunocytochemistry (IF-cell)
Species: Mouse
Sample: NIH/3T3 (Mouse fibroblast)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA724808, 1/200, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: The mitochondria dyes was Mito-Tracker (Beyotime, Red). The nuclear counterstain was DAPI (Blue). -
This data was developed using HA724808, the same antibody clone in a different buffer formulation.
Application: Immunocytochemistry (IF-cell)
Species: Rat
Sample: C6 (Rat glioma cell)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA724808, 1/1,000, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: The mitochondria dyes was Mito-Tracker (Beyotime, Red). The nuclear counterstain was DAPI (Blue). -
This data was developed using HA724808, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Colon
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724808, 1/500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
This data was developed using HA724808, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Lung
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724808, 1/500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
This data was developed using HA724808, the same antibody clone in a different buffer formulation.
Application: Flow Cytometry (Intra)
Species: Human
Sample: SH-SY5Y (Human neuroblastoma cells)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA724808 (1/500, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.
-
This data was developed using HA724808, the same antibody clone in a different buffer formulation.
Application: Flow Cytometry (Intra)
Species: Mouse
Sample: NIH/3T3 (Mouse fibroblasts)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA724808 (1/500, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.
-
This data was developed using HA724808, the same antibody clone in a different buffer formulation.
Application: Flow Cytometry (Intra)
Species: Rat
Sample: C6 (Rat glioma cells)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA724808 (1/500, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.
-
This data was developed using HA724808, the same antibody clone in a different buffer formulation.
Immunoprecipitation (IP)
LONP1 was immunoprecipitated in 0.2 mg HeLa (Human cervix adenocarcinoma epithelial cell) cell lysate with HA724808 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724808 at 1/2,000 dilution. Anti-Rabbit IgG for IP, AlpSdAbs® VHH(HRP) (025-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (input)
Lane 2: HA724808 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of HA724808 in HeLa cell lysate
Exposure time: 10 seconds
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary dilution: HA724808, 1/2,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature
Predicted band size: 106 kDa
Observed band size: 106 kDa -
This data was developed using HA724808, the same antibody clone in a different buffer formulation.
Immunoprecipitation (IP)
LONP1 was immunoprecipitated in 0.2 mg NIH/3T3 (Mouse fibroblast) cell lysate with HA724808 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724808 at 1/2,000 dilution. HRP Conjugated Rabbit IgG kappa light chain Mouse Monoclonal Antibody(M1208-2) at 1/5000 dilution was used for 1 hour at room temperature.
Lane 1: NIH/3T3 cell lysate (input)
Lane 2: HA724808 IP in NIH/3T3 cell lysate
Lane 3: Rabbit IgG instead of HA724808 in NIH/3T3 cell lysate
Exposure time: 46 seconds
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary dilution: HA724808, 1/2,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature
Predicted band size: 106 kDa
Observed band size: 106 kDa
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