CD133 Recombinant Rabbit Monoclonal Antibody [PSH26-71] - BSA and Azide free
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Catalog# HA752039
CD133 Recombinant Rabbit Monoclonal Antibody [PSH26-71] - BSA and Azide free
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WB
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IHC-P
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FC
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Human
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HA724636
含抗保成分
-
unconjugated
概述
产品名称
CD133 Recombinant Rabbit Monoclonal Antibody [PSH26-71] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
种属反应性
Human
验证应用
WB, IHC-P, FC
靶点分子量
Predicted band size: 97 kDa
阳性对照
Caco-2 (Human colorectal adenocarcinoma cell) cell lysate, HT-29 (Human colorectal adenocarcinoma cell) cell lysate.
偶联
unconjugated
克隆号
PSH26-71
反应性数据
| WB | IHC-P | FC | |
|---|---|---|---|
| Human |
|
|
|
产品特性
形态
Liquid
浓度
存放说明
Store at 2-8℃. Avoid freeze.
存储缓冲液
PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
靶点
功能
CD133 antigen, also known as prominin-1, is a glycoprotein that in humans is encoded by the PROM1 gene. It is a member of pentaspan transmembrane glycoproteins, which specifically localize to cellular protrusions. When embedded in the cell membrane, the membrane topology of prominin-1 is such that the N-terminus extends into the extracellular space and the C-terminus resides in the intracellular compartment. The protein consists of five transmembrane segments, with the first and second segments and the third and fourth segments connected by intracellular loops while the second and third as well as fourth and fifth transmembrane segments are connected by extracellular loops. While the precise function of CD133 remains unknown, it has been proposed that it acts as an organizer of cell membrane topology.
背景文献
1. Kim MY et al. Accumulation of low-dose BIX01294 promotes metastatic potential of U251 glioblastoma cells. Oncol Lett 13:1767-1774 (2017).
2. Xi G et al. Targeting CD133 improves chemotherapeutic efficacy of recurrent pediatric pilocytic astrocytoma following prolonged chemotherapy. Mol Cancer 16:21 (2017).
亚细胞定位
Endoplasmic reticulum. Plasma membrane. Cell projection.
UNIPROT
别名
AC133 antibody
Antigen AC133 antibody
CD133 antibody
CORD12 antibody
Hematopoietic stem cell antigen antibody
hProminin antibody
MCDR2 antibody
MSTP061 antibody
OTTHUMP00000217744 antibody
OTTHUMP00000217745 antibody
展开图片
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☑ Relative expression (RE)
This data was developed using HA724636, the same antibody clone in a different buffer formulation.
Western blot analysis of CD133 on different lysates with Rabbit anti-CD133 antibody (HA724636) at 1/1,000 dilution.
Lane 1: Caco-2 (Human colorectal adenocarcinoma cell) cell lysate
Lane 2: 293T (Human embryonic kidney cell) cell lysate
Lane 3: HT-29 (Human colorectal adenocarcinoma cell) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 1 minute 34 seconds; ECL: K1802
293T is a negative control (PMID: 30783186).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724636, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 97 kDa
Observed band size: 120 kDa -
This data was developed using HA724636, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Kidney
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724636, 1/500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
This data was developed using HA724636, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Spleen
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724636, 1/500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
☑ Relative expression (RE)
This data was developed using HA724636, the same antibody clone in a different buffer formulation.
Application: Flow Cytometry
Species: Human
Sample: 293T (Human embryonic kidney cells) (Left, Negative control) / Caco-2 (Human colorectal adenocarcinoma cell) (Right)
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA724636 (1/500, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.
Negative control: 293T (PMID: 30783186).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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