IL-8 Recombinant Rabbit Monoclonal Antibody [PSH10-96] - BSA and Azide free
Catalog# HA751386
IL-8 Recombinant Rabbit Monoclonal Antibody [PSH10-96] - BSA and Azide free
-
WB
-
FC
-
Human
-
HA723263
含抗保成分
-
unconjugated
概述
产品名称
IL-8 Recombinant Rabbit Monoclonal Antibody [PSH10-96] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human IL-8 aa 1-99.
种属反应性
Human
验证应用
WB, FC
分子量
Predicted band size: 11 kDa
阳性对照
U-937 treated with 100ng/mL TPA for 24 hours then add 5μg/mL LPS for 4 hours then add 300ng/mL BFA for 3 hours cell lysate, U-937 treated with 100ng/mL TPA for 24 hours then add 5μg/mL LPS for 4 hours then add 300ng/mL BFA for 3 hours.
偶联
unconjugated
克隆号
PSH10-96
产品特性
形态
Liquid
浓度
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:2,000
-
FC
-
1:1,000
靶点
功能
Interleukin 8 (IL-8 or chemokine (C-X-C motif) ligand 8, CXCL8) is a chemokine produced by macrophages and other cell types such as epithelial cells, airway smooth muscle cells and endothelial cells. Endothelial cells store IL-8 in their storage vesicles, the Weibel–Palade bodies. In humans, the interleukin-8 protein is encoded by the CXCL8 gene. IL-8 is initially produced as a precursor peptide of 99 amino acids which then undergoes cleavage to create several active IL-8 isoforms. In culture, a 72 amino acid peptide is the major form secreted by macrophages. There are many receptors on the surface membrane capable of binding IL-8; the most frequently studied types are the G protein-coupled serpentine receptors CXCR1 and CXCR2. Expression and affinity for IL-8 differs between the two receptors (CXCR1 > CXCR2). Through a chain of biochemical reactions, IL-8 is secreted and is an important mediator of the immune reaction in the innate immune system response.
背景文献
1. Ramachandra N et al. Role of IL8 in myeloid malignancies. Leuk Lymphoma. 2023 Nov-Dec
2. Jiang H et al. Targeting IL8 as a sequential therapy strategy to overcome chemotherapy resistance in advanced gastric cancer. Cell Death Discov. 2022 Apr
亚细胞定位
Secreted.
UNIPROT
别名
(Ala-IL-8)77 antibody
(Ser-IL-8)72 antibody
9E3 antibody
Beta thromboglobulin like protein antibody
C-X-C motif chemokine 8 antibody
CEF-4 antibody
chemokine, CXC motif, ligand 8 antibody
CXCL8 antibody
Emoctakin antibody
GCP-1 antibody
展开图片
-
☑ Cell treatment (CT)
Western blot analysis of IL-8 on different lysates with Rabbit anti-IL-8 antibody (HA751386) at 1/2,000 dilution.
Lane 1: U-937 cell lysate
Lane 2: U-937 treated with 100ng/mL TPA for 24 hours then add 5μg/mL LPS for 4 hours then add 300ng/mL BFA for 3 hours cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 11 kDa
Observed band size: 11 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751386) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Flow cytometric analysis of U-937 cells untreated (left) / treated with 100ng/mL TPA for 24 hours then add 5μg/mL LPS for 4 hours then add 300ng/mL BFA for 3 hours (right) labeling IL-8.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA751386, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
IL-8 Recombinant Rabbit Monoclonal Antibody [PSH10-96]
Application: WB,FC
Reactivity: Human
Conjugate: unconjugated
Human IL-8 Recombinant Rabbit Monoclonal Antibody [PS00-83] - BSA and Azide free (Capture)
Application: ELISA(Cap)
Reactivity: Human,Dog,Cynomolgus monkey
Conjugate: unconjugated
IL-8 Rabbit Polyclonal Antibody
Application: WB,IHC-P,IF-Cell,FC
Reactivity: Human
Conjugate: unconjugated
Human IL-8 Recombinant Rabbit Monoclonal Antibody [PS01-22] - BSA and Azide free (Detector)
Application: ELISA(Det)
Reactivity: Human,Dog,Cynomolgus monkey
Conjugate: unconjugated
IL-8 Rabbit Polyclonal Antibody
Application: WB,IHC-P,IF-Cell,FC
Reactivity: Human
Conjugate: unconjugated
IL-8 Recombinant Rabbit Monoclonal Antibody [PSH10-97]
Application: WB
Reactivity: Human
Conjugate: unconjugated
IL-8 Recombinant Rabbit Monoclonal Antibody [PSH10-97] - BSA and Azide free
Application: WB
Reactivity: Human
Conjugate: unconjugated
Biotin Conjugated Human IL-8 Recombinant Rabbit Monoclonal Antibody [PS01-22]
Application: ELISA(Det)
Reactivity: Human,Dog,Cynomolgus monkey
Conjugate: Biotin
浙公网安备 33019202000643号